Annunziata, A. L.

Colimoro, L.

Cosenza, G.

Gallo, D.

Mancusi, A.

Pauciullo, Alfredo

Ramunno, L.

English

Institutional Research Information System University of Turin

ISSN 1594-4077Vol.8 - 2OO9Supplement 2www.aspajournal. itITnLIANJOURNALOFANIMALScrENcEJournal and Official Organof the Scientific Association of Animal Production (ASPA)THE ASPA(bulJEdizioniAvenue media'Milano -BolognaGene structure anarysis of caprine andovine Mannose Binàing Lectin rrrreLl-.Gianfranco cosenza, Arfredo pauciuilo, Letizia corimoro,Andrea Mancusi, Anna Lidia Annunziata,Daniela Gallo, Luigi RamunnoDipar[imento dÌ scienze del suolo, della Pianta, dellAmbiente e delle produzioni AnimaliUniversità di Napoli ,,Federico II", ItalycoTesponcÌing auihor" Luigr Ramunno Diparrimento di scienze del suo10. della pianta. deilAmbiente edelle Produzioni -{nrmah universrtà di Napoli "Federico II". \iia u""..Ja 100. 80055 portici (NA). Italv- Tel. +39 0S1 25S900.1 - Fax: +39 OSf ;Z.ZÉSO _ Email: .u_ro.roO,r.riou:tABSTRACTThe Mannose Binding Lectin (MBL) is an importaat factor of natural immunity r.vhich acts as aprimordial antibodr', capable to acti'ate the complement and to link to some sugars (such as man-nose) present on the surface of some bacteria as Brucelia. L thu p."*rt stud1.. we report the partialstructure of the gene coding for the MBL in sheep and goat as weil as the a,alysis of their promot-ers' By using as template genomic DNA from leukoc5.-tes obtained from indi'idual blood samples wesequenced from -961 nt of the promoter to the 397th nt of the 4,h exon of fbur sheep (EMBLAcc. no.4M933378) and from -97g nt to the 471th nt of the 4th exon of to,,,, gort. (EMBL Acc. no. AMg33377),for a total of 4467 bp and 4515 bp, respectivellr 85.'comparing rrr" rr"o*oìogous sequence of the bo'ineMBL gene (EMBLAcc no NC007327),itrvas possible to determine the size of the exons, similar inboth investigated species:200 bp (exon 1), 117 Lp (exon 2). and 69 bp (exon 3). The comparison ofthesequences betrveen goat and sheep shor.r,s an homoiogl- of g2.2Tc.The differences are due essentialÌyto insertions/deletions at intronic level: the sheep presents r-ith respect to the goat a deletion at the1"'intron of 11 nt' t*o insertrons at the 2"d intron. respectivelv of 29 and 41 nt, and at the 3.d introna stretch of eighr Guanrae rn tbe eoat r.s eiohr lu,as 3e5 bp us 385 r,"*"-,lr-"r" o'J::'Jl?:i.1:ffi,'Jr'il;tr,Jil#?flil1:T"lJn,T[:l;goatandsheep Forbothspecies.allthesplicejunftionsfoilou-the.oo.-o.,role5,GT/3,AG.TheoRF(open Reading Frame) encodes for 249 amino acid residues. u,hereas the Ìeader peptide is composedby 19 amino acids The startrng codon (ATG, Methionine) is located between the 10to and the 12tr nt o{.the 1"i exon' in'hile the stop codon (TGA) bet'u,'een the B72ih a..d the 374th nt of the 4th exon. \\4rile fromthe comparison of the exonic sequences, 23 sNPs were identified, 10 oirrhich give rise to an aminoacidic change The sequence analysis of the promoter region in sheep shou,s the presence of the f.oi-lowing putative binding sites for transcription factors: TATA_box (ATA_$) and CCA*A.T_box (CCA-AAT)in position -49 and -140 nt, respectivelv'. Furthermore, ;;;r*;.,o..iloi .".poo=ive erements (GR_EJwere identified: in position -765 (AGATCAGA) and -971 (ACiGATcD. irre promorer anarysis for goatwas found to be analogous to that ofsheep but it shows the disappearrrr." orrrr. reg,ìatory site GRE(ACTGATCT). Further studies need to be performed in order to verifi, whether the markers identifiedin the present study can be associated to ìhe resistance to pathogens such as Brucella melitensis bvcarrying out suitable test for antimicrobial actir_itr,.196IraL.J.Ar,irM.Scr. vor. B (Suecr, 2), 2OOg

Gene structure analysis of caprine and ovine Mannose Binding Lectin (MBL)

https://iris.unito.it/bitstream/2318/1507145/1/MBL%20-%20IJAS%202009.pdf

Gene structure analysis of caprine and ovine Mannose Binding Lectin (MBL)

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