Micky Vincent1,*, Latifah Suali1, Afizul Safwan Azahari1, Patricia Rowena Mark Baran1, Elexson

Nillian1, Lesley Maurice Bilung

AfIzul Safwan, Azahari

Elexson, Nillian

Latifah, Suali

Lesley Maurice, Bilung

Micky, Vincent

Patricia Rowena, Anak Mark Baran Saging

Unimas Institutional Repository

 Journal of Advanced Research in Applied Sciences and Engineering Technology 11, Issue 1 (2018) 47-54 47   Journal of Advanced Research in Applied Sciences and Engineering Technology Journal homepage: www.akademiabaru.com/araset.html ISSN: 2462-1943  Growth Kinetics of Ethidium Bromide Mutagenized Lipomyces starkeyi Strains   Micky Vincent1,∗, Latifah Suali1, Afizul Safwan Azahari1, Patricia Rowena Mark Baran1, Elexson Nillian1, Lesley Maurice Bilung1  1 Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia   ARTICLE INFO ABSTRACT Article history: Received 17 January 2018 Received in revised form 28 February 2018 Accepted 2 May 2018 Available online 13 May 2018 Yeast growth and biomass production are greatly influenced by the length of the incubation period during cultivation. Therefore, this study was conducted to investigate the growth kinetics of five Lipomyces starkeyi strains as determined by biomass production. The five L. starkeyi strains, namely L. starkeyi ATCC 12659, L. starkeyi MV-1, L. starkeyi MV-4, L. starkeyi MV-5 and L. starkeyi MV-8, were inoculated in sterilized Yeast Malt broth, and, incubated for 192 hr at ambient temperature. Biomass yields were assessed and calculated gravimetrically every 24 hr. Results indicated that the optimal biomass production of L. starkeyi ATCC 12659, L. starkeyi MV-1, L. starkeyi MV-4, L. starkeyi MV-5 and L. starkeyi MV-8 were at 120, 168, 144, 168 and 120 hr, with the concentrations of 6.64, 6.43, 9.78, 11.23 and 8.56 g/L, respectively. These results indicate that each L. starkeyi strain requires specific incubation period for the optimum production of fungal biomass. Therefore, by cultivating each L. starkeyi strain at the predetermined incubation period, biomass yields could significantly be improved for further downstream applications such as single cell protein and lipid production. Keywords:  Lipomyces starkeyi, random mutagenesis, oleaginous yeasts, growth kinetics, fungal biomass Copyright © 2018 PENERBIT AKADEMIA BARU - All rights reserved  1. Introduction  Yeasts are known for their high biomass production and rapid growth rates [1]. They are capable of producing high biomass yields of between 10 to 100 g/L after 3 to 7 days of incubation period [2]. The biomass produced from these unicellular fungi is valuable because of its nutritional values, non-toxic properties and low nucleic acid content [3]. Therefore, yeasts are extensively used in various sectors of the agricultural industry as food supplements for human and animals, production of biofuels, beverages, bakery and probiotics [4-5]. Many yeasts are oleaginous. One example is Lipomyces starkeyi, which is reported to produce lipids up to 70% of its dry biomass under optimum cultivation conditions [6-8]. These lipids are mainly                                                             ∗ Corresponding author. E-mail address: Micky Vincent (vmicky@unimas.my) PenerbitAkademia BaruOpen Access 

Growth Kinetics of Ethidium Bromide Mutagenized Lipomyces

starkeyi Strains

Yeast and growth and biomass production are greatly influenced by the length of the incubation period during cultivation. Therefore, this study was conducted to investigate the growth kinetics of five Lipomyces starkeyi strains as determined by biomass production. The five L. starkeyi strains, namely L. starkeyi ATCC 12659, L. starkeyi MV-1, L. starkeyi MV-4, L. starkeyi MV-5 and L. starkeyi MV-8, were inoculated in sterilized Yeast Malt broth, and, incubated for 192 hr at ambient temperature. Biomass yields were assessed and calculated gravimetrically every 24 hr. Results indicated that the optimal biomass production of L. starkeyi ATCC 12659, L. starkeyi MV-1, L. starkeyi MV-4, L. starkeyi MV-5 and L. starkeyi MV-8 were at 120, 168, 144, 168 and 120 hr, with the concentrations of 6.64, 6.43, 9.78, 11.23 and 8.56 g/L, respectively. These results indicate that each L. starkeyi strain requires specific incubation period for the optimum production of fungal biomass. Therefore, by cultivating each L. starkeyi strain at the predetermined incubation period, biomass yields could significantly be improved for further downstream applications such as single cell protein and lipid production

Lesley, Maurice Bilung

Patricia Rowena, Mark Baran Saging

Growth Kinetics of Ethidium Bromide Mutagenized Lipomyces starkeyi Strains

http://ir.unimas.my/id/eprint/21677/1/Growth%20Kinetics%20of%20Ethidium%20Bromide%20Mutagenized%20Lipomyces%20%28abstract%29.pdf

Growth Kinetics of Ethidium Bromide Mutagenized Lipomyces starkeyi Strains

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