Malaysian Society for Molecular Biology & Biotechnology
Abstract
An in vitro propagation system was developed for Globba brachyanthera K.Schum., a potential ornamental plant by surface sterilized adventitious bulbils in 20% Clorox for 20 minutes and cultured on Gamborg B5 medium supplemented with a biocide Plant Preservative Mixture (PPM) and antibiotic tetracycline. Shoot tips (2-5mm) obtained from in vitro cultured plantlets were induced to form shoots on Gamborg B5 medium containing 20% sucrose and 2.8 g/L Gelrite supplemented with various concentrations of 6-benzylaminopurine (BAP) ranging from 1.0 to 3.0mg/L, either individually or in combination with alpha-naphthalene acetic acid (NAA) at 0.1 mg/L or 0.5 mg/L. All treatments induced formation of multiple shoots as well as rooting after 8 weeks of culture. The highest multiplication rate of 6.6 shoots per explants was obtained in Gamborg B5 medium supplemented with 3.0 mg/L BAP. The generated shoots elongated on Gamborg B5 medium and the multiplication rate did not change further in all of the successive subcultures
