Scientific name for green-spotted puffer is Tetraodon nigroviridis and this species belongs to the family of
Tetraodontidae. T. nigroviridis is distributed along the brackish water, mangrove area, river and flood plains. T.
nigroviridis is importance in genomic study and also famous as ornamental fish. The morphology of T.
nigroviridis was examined according to their body weight, standard length and total length. Range of body
weight for male T. nigroviridis is 74.92±30.1 g and for female is 106.00±36.96 g. In larvae production, T.
nigroviridis was cultured in the laboratory and the artificial breeding was induced by Ovaprim hormone. Ovaprim
hormone was used in ornamental finfish brood stock only and it was injected according to the fish body weight.
Chromatography analyses (TLC, HPLC and LC-MS) were carried out to analyse the toxic level in the different
tissues of brood stock of T. nigroviridis. Among the tissues, egg was found to have the highest concentration of
TTX with the result 1330.91 MU/g, followed by intestine and muscle. The baseline data for culturing T.
nigroviridis in captivity can be used as a guideline for larvae production in the future. On the other side, the TTX
data in T. nigroviridis tissues could be important information and can be used in order to mitigate puffer fish
poisoning cases especially in East Malaysia water
