The SMC complex, MukBEF, is important for chromosome organization and
segregation in Escherichia coli. Fluorescently tagged MukBEF forms distinct
spots (or 'foci') in the cell, where it is thought to carry out most of its
chromosome associated activities. This chapter outlines the technique of
Fluorescence Recovery After Photobleaching (FRAP) as a method to study the
properties of YFP-tagged MukB in fluorescent foci. This method can provide
important insight into the dynamics of MukB on DNA and be used to study its
biochemical properties in vivo
