Belcari, Antonio

Bigiotti, Gaia

Roberta, Pastorelli

Roberto, Guidi

Sacchetti, Patrizia

English

Florence Research

BOOK OF ABSTRACTSXI EUROPEAN CONGRESS OF ENTOMOLOGY2-6 JULY 2018, NAPOLIAccademiaNazionale Italianadi Entomologia                                                                                                   329Impact of Symbionts on Insect BiologyPO559genome reduction in the mosquito symbiont ASAIADiego Alonso, Biotechnology Institute (IBTEC) & Biosciences Institute at Botucatu (IBB), Sao Paulo State University (UNESP), Sao Paulo, BrazilMaria Vittoria Mancini, School of Biosciences and Veterinary Medicine, University of Camerino, ItalyClaudia Damiani, School of Biosciences and Veterinary Medicine, University of Camerino, ItalyAlessia Cappelli, School of Biosciences and Veterinary Medicine, University of Camerino, ItalyIrene Ricci, School of Biosciences and Veterinary Medicine, University of Camerino, ItalyMarcus Vinicius - Biotechnology Institute (IBTEC) & Biosciences Institute at Botucatu (IBB), Sao Paulo State University (UNESP), Sao Paulo, BrazilPaulo Ribolla - Biotechnology Institute (IBTEC) & Biosciences Institute at Botucatu (IBB), Sao Paulo State University (UNESP), Sao Paulo, BrazilGuido Favia - School of Biosciences and Veterinary Medicine, University of Camerino, ItalyThe genus Asaia represents a peculiar member of Acetic acid bacteria (AAB): it oxidizes acetate and lactate to carbon dioxide and water, but notethanol to acetic acid. Several species and strains of the genus Asaia were firstly isolated from tropical flowers. The symbiotic mutualism betweenAsaia and mosquitoes. encompassing Anopheles, Aedes and Culex genera, well-known vectors of parasites and arboviruses causing some of themajor infectious diseases in public health, including malaria, Dengue and Zika is widely accepted and its colonization within its hosts is characterizedby a wide tissue tropism, harbouring in the gut, the salivary glands and the reproductive tracts of female and male individuals, corresponding to themode of its horizontal transmission (co-feeding) within a population and its vertical transmission between host generations (paternal transmission).Studies aimed at understanding the features of AAB symbiotic alliances with their hosts focused on the investigation of possible benefits providedby the role of the potential advantages given by AAB to their respective hosts. Through the genome sequencing of several Asaia isolates from variousmosquito species, we disclosed differences in the architecture of the strain isolated from field-collected specimens of the South-American malariavector Anopheles darlingi, and in particulary its genome reduction in comparison with other isolates from mosquito species belonging to Anophelesand Aedes genera. Thus Asaia, similarly to what proposed for Serratia symbiotica (seems to be a good model to study the genome reduction dynamicswithin a single bacterial taxon evolving in the mosquito host that represents a common biological niche.Keywords: Asaia, endosymbiont, genome reductionPO560effects of lab Procedures on the transfer of B. OLEAE endosymbiont, CANDIDATUS ERWINIADACICOLAGaia Bigiotti, Department of Agrifood Production and Environmental Sciences (DISPAA), University of Florence, Florence, ItalyPatrizia Sacchetti, Department of Agrifood Production and Environmental Sciences (DISPAA), University of Florence, Florence, ItalyRoberta Pastorelli, Research Centre for Agriculture and Environment (CREA-AA), Florence, ItalyRoberto Guidi, Department of Agrifood Production and Environmental Sciences (DISPAA), University of Florence, Florence, ItalyAntonio Belcari, Department of Agrifood Production and Environmental Sciences (DISPAA), University of Florence, Florence, ItalyBactrocera oleae (Rossi) is a key pest of the olive crops in the Mediterranean basin. An important issue regarding B. oleae on which scientists arefocusing the most is its symbiosis with Candidatus Erwinia dacicola. This bacterium is considered essential for the olive fly. It is vertically transmittedthrough generations and it benefits both larvae and adults in field; whereas, it had been found rarely in lab colonies, probably because of preservativesin artificial diets. So that, in order to understand the reason of its loss during rearing lab procedures, we exposed eggs laid by wild females to threedifferent treatments: a propionic acid solution, a mixture of sodium hypochlorite plus Tritonxand water as control. The symbiont load on eggs wasevaluated by real-time PCR and SEM. In the same time, we also tried a horizontal transfer among adults, exposing lab flies to ripe olives and gelledwater contaminated by wild flies, wax domes containing eggs laid by wild females, cages dirtied by faeces dropped by wild flies in cohabitation withwild adults. In the first experiment, real-time PCR of eggs showed a significant reduction in Ca. E. dacicola abundance on eggs treated with propionicacid solution or the mixture of sodium hypochlorite plus Tritonxcompared to the control. Secondly, PCR-DGGE highlighted that the horizontal transferof the endosymbiont occurred in the case of cohabitation. Thus, our results indicate how the symbiont load could be decreased by the use ofpreservatives in lab rearing procedures and that cohabitation between wild flies and lab ones permits the horizontal transfer opening further possibilitiesto establish a permanent symbiotic colony, a strategic tool for future SIT applications. Moreover, PCR-DGGE performed with an appropriate primerset was proved to be a consistent method for Ca. E. dacicola screening.Keywords: Olive fly, lab rearing, endosymbiont, Candidatus Erwinia dacicola, DGGE, SITPO561differential gene exPression in wolbachia infected and non-infected DROSOPHILA PAULISTORUMGuilherme Costa Baiao, Department of Cell and Molecular Biology, Uppsala University, SwedenDaniela Schneider, Department of Cell & Developmental Biology, Medical University of Vienna, AustriaWolfgang Miller, Department of Cell & Developmental Biology, Medical University of Vienna, AustriaLisa Klasson, Department of Cell and Molecular Biology, Uppsala University, SwedenThe neotropical superspecies Drosophila paulistorum (Diptera, Insecta) comprises at least six reproductively isolated forms, each harboring a closelyrelated but bidirectionally incompatible mutualistic Wolbachia strain. Although wild type flies of each semispecies are known to be isolated from theothers by both pre- and post mating incompatibilities, recent studies have shown that mating and successful offspring development can be achievedafter insects are treated for reduction of Wolbachia titer. This suggests that in this system the bacteria not only prevents embryonic development throughcytoplasmic incompatibility but also affect mate choice, possibly through interference in pheromone production and recognition. In the present study,RNA samples were collected from heads and abdomens of males and females of three semispecies of D. paulistorum. Both naturally infected wild typespecimens and flies which had gone through antibiotics treatment and subsequent gut flora restoration were sampled so that the influence of Wolbachiaon gene expression could be investigated. Differential gene expression analysis was done using reads mapped to a de-novo assembled transcriptomeand revealed hundreds of genes with altered expression after Wolbachia depletion. Several of these were involved in cell cycle and germ cell development,which possibly relates to Wolbachia’s tropism for reproductive tissue and ability to maniputale host reproduction. However, a large number of genesrelated to other processes were also observed, including stress response, defense against microbes, pheromone production and reception, fatty acidmetabolism, proteolysis, muscular and visual functions, among others which might be related to less studied phenotypes induced by the symbiont.Keywords: Wolbachia, Drosophila, symbiosisXI European Congress of Entomology, Napoli 2-6 July 2018

Effects of lab procedures on the transfer of B. oleae endosymbiont, Candidatus Erwinia dacicola

https://flore.unifi.it/bitstream/2158/1131813/1/Bigiotti%20et%20al._ECE%202018.pdf

Effects of lab procedures on the transfer of B. oleae endosymbiont, Candidatus Erwinia dacicola

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