Bakonyi, J

Carella, G

Cravador, A

Duran, A

Fajardo, S

Gonzales, M

Horta Jung, M.

Jung, T

Maia, C

Mihn, Cn

Moricca, Salvatore

Mosca, S.

Perez Sierra, A

Rees, H

Sala, Bm

Scanu, B

Schena, L.

Seress, D

Thu, Pq

von Stowasser ES,

English

Florence Research

 OMGN 2017  March 11 - 14, Asilomar Conference Grounds       	 26	 Monday, March 13th   3:50 - 5:10 Oomycete biology, populations, and evolution I Chair: Javier F. Tabima 3:50 - 4:05 Phylogeography of the tropical oomycete Phytophthora palmivora Erica M. Goss 4:10 - 4:25 Diversity of Phytophthora species from natural and semi-natural ecosystems in Portugal, Chile and Vietnam Marília Horta Jung 4:30 - 4:45 Live cell imaging of the cytoskeleton in Phytophthora pathogens reveals unique actin and microtubule configurations Kiki Kots 4:50 - 5:05 Morphological and molecular identification of Phytophthora palmivora Butler as causal agent of black pod rot of cocoa (Theobroma cacao L.) from coastal Ecuador Miriam Escos  Phylogeography of the tropical oomycete Phytophthora palmivora Jianan Wang1*, Michael D. Coffey2, Erica M. Goss1,3 1 Department of Plant Pathology, University of Florida, Gainesville, Florida, USA; 2 Department of Plant Pathology and Microbiology, University of California, Riverside, California, USA; 3 Emerging Pathogen Institute, University of Florida, Gainesville, Florida, USA; * Current address: Department of Agronomy, Kansas State University, Manhattan, Kansas, USA  The genetic structure and diversity of oomycetes are shaped by their evolutionary history, including coevolutionary interactions with hosts, geographic spread, and long-distance migration events, often mediated by humans. In turn, diversity and dispersal of present populations impact the risk of disease epidemics and strategies for controlling disease. Phytophthora palmivora is a globally distributed oomycete that infects a broad range of cash crops and fruit trees in the tropics and subtropics, including cocoa and palms. The center of diversity of P. palmivora is in Southeast Asia, but it is an important and widespread pathogen in South America. Our multilocus sequence analysis showed that the centers of origin of the pathogen are located in the Philippines and Indonesia. We then tested alternative models for the emergence of P. palmivora. We found genetic variation consistent with historical movement among Pacific Islands, likely associated with coconut. The population structure of P. palmivora is consistent with a bridgehead effect, such that the colonization of South America and host shift to cocoa led to further global dispersal, including gene flow back to Southeast Asia. We propose that the extensive genetic diversity in P. palmivora in Southeast Asia is the result of a complex history, including long-term co-evolution with native hosts, geographic isolation with migration, and re-introduction of genotypes from South America.   Diversity of Phytophthora species from natural and semi-natural ecosystems in Portugal, Chile and Vietnam Thomas Jung1,2,3, Bruno Scanu4, József Bakonyi 5, Diána Seress 5, Alvaro Durán 6, Eugenio Sanfuentes von Stowasser 7, Leonardo Schena8, Saveria Mosca 8, Pham Quang Thu9, Chi Nguyen Minh9, Sebastian Fajardo7, Mariela González 7, Ana Pérez-Sierra 10, Helen Rees 10, Cristiana Maia1, Beatriz Mora Sala11, Giuseppe Carella12, Salvatore Moricca12, Alfredo Cravador1, Marília Horta Jung1,2 (1Laboratory of Molecular Biotechnology and Phytopathology, Center for Mediterranean Bioresources and Food (MeditBio), University of Algarve, Faro, Portugal; 2Phytophthora Research Centre, Mendel University, Brno, Czech Republic; 3Phytophthora Research and Consultancy, Nußdorf, Germany; 4Dipartimento di Agraria, Sezione di Patologia vegetale ed Entomologia (SPaVE), Università degli Studi di Sassari, Sassari, Italy; 5Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary; 6Ontario Forest Research Institute, Sault Ste. Marie, Canada; 7Laboratorio de Patología Forestal, Facultad Ciencias Forestales y Centro de Biotecnología, Universidad de Concepción, Concepción, Chile; 8Dipartimento di Gestione dei Sistemi Agrari e Forestali, Università Mediterranea di Reggio Calabria, Reggio Calabria, Italy; 9Forest Protection Research Centre, 	 27	Vietnamese Academy of Forest Sciences, Duc Thang Ward, Northern Tu Liem District, Hanoi, Vietnam; 10Forest Research, Alice Holt Lodge, Farnham, Surrey, United Kingdom; 11Instituto Agroforestal Mediterráneo, Universitat Politècnica de Valencia, Edificio 3H, Camino de Vera s/n, Valencia, Spain; 12Department of Agri-Food Production and Environmental Sciences, Plant Pathology and Entomology Division, University of Florence, Piazzale delle Cascine, Florence, Italy)  Surveys of Phytophthora diversity were performed using baiting assays and direct plating of necrotic plant tissues. Isolates were identified using both classical identification and sequence analysis of ITS and cox1. In Portugal, 68 forest stands, 12 forest nurseries, 38 river systems and 4 lagoon ecosystems were surveyed. The isolates obtained belonged to 29 known species, 3 informally designated taxa, and 1 previously unknown taxon of Phytophthora, nine new Phytophthora hybrid taxa from Clades 6 and 9, Nothophytophthora homothallica nom. prov., 1 known and 10 new Halophytophthora species, 7 known species and one new taxon of Phytopythium, and multiple Pythium species were isolated. In Chile, the survey was performed in 13 natural forest stands and 20 forest streams located in two protected areas near Valdivia and in a temperate mountain forest in the Concepción area, and in each one planted stand of the introduced tree species Castanea sativa and Fagus sylvatica. Eight described species (including P. kernoviae) and 2 previously unknown taxa of Phytophthora were isolated. In addition, a diverse array of Clade 6 hybrids, and Nothophytophthora caduca nom. prov., Nothophytophthora chlamydospora nom. prov. and Nothophytophthora valdiviana nom. prov., were obtained. In Vietnam the survey was performed in 23 natural forest stands and 10 forest streams and rivers in temperate montane and tropical lowland regions, and in 14 rubber plantations. Sixteen described species (including P. ramorum), 3 designated taxa and 23 previously unknown taxa of Phytophthora, amongst them 9 Clade 9 hybrid taxa, were isolated. In addition, Nothophytophthora vietnamensis nom. prov. and a diverse array of known and new taxa of Phytopythium, Pythium and Elongisporangium were recovered. The implications of these findings for plant biosecurity and the development of a deeper understanding of the evolution and adaptability of the genus Phytophthora will be discussed.   Live cell imaging of the cytoskeleton in Phytophthora pathogens reveals unique actin and microtubule configurations Kiki Kots1,2, Tijs Ketelaar2, Johan van den Hoogen1, Harold Meijer1, Francine Govers1 (1. Laboratory of Phytopathology, University of Wageningen, Wageningen, The Netherlands; 2. Laboratory of Cell biology, Wageningen University, Wageningen, The Netherlands)  The cytoskeleton is a dynamic but well organized intracellular network that is essential for proper functioning of eukaryotic cells. We study the cytoskeleton in Phytophthora species, oomycete plant pathogens that cause devastating diseases worldwide. We use Lifeact-eGFP expressing Phytophthora infestans for live cell imaging of the actin cytoskeleton in various developmental stages. Previously we identified actin plaques as highly immobile, long-lived structures that are unique for oomycetes. Here we present two other unique actin configurations; one associated with plug deposition in germ tubes and the other with appressoria, infection structures formed prior to host cell penetration. Plugs are composed of cell wall material that is deposited in hyphae emerging from cysts to seal off the cytoplasm-depleted base after cytoplasm retraction towards the growing tip. Preceding plug formation a typical local actin accumulation was observed that remained associated with the leading edge during plug deposition. In appressoria we observed an aster-like actin configuration at the contact point with the underlying surface. These findings strongly suggest a role for the actin cytoskeleton in plug formation and plant cell penetration. For live cell imaging of the microtubule cytoskeleton we have generated a Phytophthora palmivora transformant expressing GFP-α-tubulin allowing us to visualize the dynamics of microtubules in oomycetes for the first time. The data presented here provide a better understanding of the structure and functioning of the Phytophthora cytoskeleton. The long term goal is to uncover oomycete or Phytophthora specific features in the cytoskeleton that might be instrumental for drug design.  

Diversity of Phytophthora species from natural and semi-natural ecosystems in Portugal, Chile and Vietnam

https://flore.unifi.it/bitstream/2158/1097485/1/Moricca%20OMGN_2017_Abstract-book_FINAL.pdf

Diversity of Phytophthora species from natural and semi-natural ecosystems in Portugal, Chile and Vietnam

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