Biancalani, Carola

Calamai, Silvia

Cerboneschi, Matteo

Osdaghi, E.

Tegli, Stefania

English

Florence Research

An International Journal of the Italian Society for Plant PathologyJournal of Plant PathologyFormerly    Rivista di patologia vegetale established in 1892EDIZIONI ETS, Pisa, Italy ISSN 1125-4653Poste Italiane S.p.A. - Spedizione in Abbonamento Postale - D.L. 353/2003 (conv. in L. 27/02/2004 n° 46) art. 1, comma 1 - DCB AREA CENTRO 1/Pisa.Volume 99 (Supplement) October 2017Piacenza, October 4-6, 2017Abstracts of papersXXIII NATIONAL MEETINGOF THE ITALIAN SOCIETY FOR PLANT PATHOLOGY (SIPaV) JPP 99 (supp.).indb   1 22/09/17   10:06Journal of Plant Pathology (2017), 99 (Supplement), Posters S41favorable conditions first occur at late anthesis, F. poae may have a relative lead in provoking the disease.FUSARIUM SPECIES AND SECONDARY METABOLITES ASSOCIATED WITH DURUM WHEAT GRAINS FROM THREE DIFFERENT ITALIAN CLIMATIC AREAS. G. Bec-cari1, M.T. Senatore1,2, L. Pedini1, F. Tini1, A. Prodi2, P. Nipoti2, V. Balmas3, M. Sulyok4, L. Covarelli1. 1 Department of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy. 2 Department of Agricultural Sci-ences, Alma Mater Studiorum University of Bologna, Viale G. Fanin, 44, 40127 Bologna, Italy. 3 Department of Agriculture, University of Sassari, Via E. De Nicola 9, I-07100 Sassari, Italy. 4 Department of Agrobiotechnology, University of Natural Resources and Life Scienc-es, Vienna, Konrad Lorenzstraße 20, A-3430 Tulln, Austria. E-mail: lorenzo.covarelli@unipg.itDurum wheat grain samples, collected in three different Italian climatic areas (Emilia-Romagna, Umbria and Sardinia), were ana-lyzed to: 1) isolate the mycoflora infecting the seeds on modified potato dextrose agar and by the deep-freezing blotter method; 2) identify the composition of the Fusarium head blight (FHB) species complex by TEF1-α region sequencing; 3) quantify the Fusarium species associated with FHB by q-PCR directly in the kernels; 4) determine fungal secondary metabolites in the grains by LC-MS/MS. The mycoflora was mainly represented by Alternaria and Fu-sarium species. The highest Fusarium spp. incidence was found in the samples from Emilia-Romagna, followed by those harvested in Umbria and in Sardinia. The FHB complex was mainly represented by F. poae in all the examined areas, while F. graminearum showed the highest incidence in the Emilia-Romagna samples followed by those from Umbria. This species was not detected in the samples from Sardinia. q-PCR assays allowed the detection of other Fu-sarium species, such as F. langsethiae and F. sporotrichioides, which were not found by the used isolation methods, showing that this technique may give an important contribution for an exhaustive description of the FHB complex. Secondary metabolites were cor-related to the fungal community detected. In fact, deoxynivale-nol (DON) was mainly found in the Emilia-Romagna samples, in which the DON producing species were more frequent. Enniatins and beauvericin were detected in the samples collected in Emilia-Romagna and Umbria. This study gives a comprehensive overview on the FHB complex by different analytical techniques and on the associated secondary metabolites.SUSCEPTIBILITY OF PAPAVERACEAE SPECIES TO FUSARIUM OXYSPORUM f. sp. PAPAVERIS. D. Bertetti1, M.L. Gullino1,2, A. Garibaldi1. 1 Università degli Studi di Torino, Centro AGROINNOVA, Largo Braccini 2, 10095 Grugliasco (TO), Italy. 2 Università degli Studi di Torino, DISAFA, Largo Braccini 2, 10095 Grugliasco (TO), Italy. E-mail: domenico.bertetti@unito.itIn 2011, Fusarium oxysporum f. sp. papaveris was reported on Iceland Poppy (Papaver nudicaule) for the first time in Italy. Suc-cessively, the susceptibility of eleven Papaveraceae species was evaluated. Nine Papaver species commonly grown for gardens and cut flowers were artificially inoculated together with P. rhoeas and Chelidonium majus naturally diffused in Italian flora. Five single-spore strains of the pathogen were used in the tests and the roots of the plants were dipped in the conidial suspensions prepared for each of them. On the basis of the disease index (D.I.) obtained by the final evaluation of symptoms, each species was assigned to one of the following susceptibility classes: R = Resistant (D.I. 0-5); PR = Partially Resistant (D.I. 6-20); MS = Moderately Susceptible (D.I. 21-50); S = Susceptible (D.I. 51-75); HS = Highly Susceptible (D.I. 76-100). Papaver atlanticum, P. dubium, P. glaucum, P. pseudo-canescens, P. nudicaule, P. rupifragum and Papaver “Daneborg” were susceptible or highly susceptible to the pathogen; P. trinifolium and P. orientale were moderately susceptible; C. majus and P. rhoeas showed a susceptibility ranging from moderate to high. About the origin of F. oxysporum f. sp. papaveris, the susceptibility of C. majus and P. rhoeas might indicate that the pathogen is present in the spontaneous flora in Italy and/or that wild Papaveraceae species are a potential source of inoculum; the high temperatures as those registered in the glasshouse cultivation where the disease first ap-peared in Italy might help the development of symptoms that are limited in the sub-polar areas in which P. nudicaule is indigenous.LOOP-MEDIATED ISOTHERMAL AMPLIFICATION AS-SAY FOR THE RAPID DETECTION OF CURTOBACTE-RIUM FLACCUMFACIENS pv. FLACCUMFACIENS FOR QUARANTINE AND FIELD APPLICATIONS. C. Biancalani1, S. Calamai1, M. Cerboneschi1, E. Osdaghi2, S. Tegli1. 1 Dipartimen-to di Scienze Produzioni Agroalimentari e dell’Ambiente (DISPAA), Laboratorio di Patologia Vegetale Molecolare, Università degli Studi di Firenze, Via della Lastruccia 10, 50019 Sesto Fiorentino (Firenze), Italy. 2 Department of Plant Protection, Shiraz University, Shiraz, Iran. Email: carola.biancalani@unifi.itCurtobacterium flaccumfaciens pv. flaccumfaciens (Cff) is a Gram-positive bacterium, and the causal agent of a systemic vas-cular disease known as bacterial wilt of beans (Phaseolus vulgaris). Bean seedlings rapidly die after infection, while older plants can survive the attack and produce mature seeds, infected by Cff via the vascular system. These seeds are generally asymptomatic, with rare yellow, orange, or purple pigmentation on white bean seeds, depending on the Cff strains/variants. Within or on infected seeds Cff remains viable for decades, and seeds are the main means of dissemination of Cff over short and long distances. This bacterium is present since the early 20th century in North America, where it still causes high economic losses, and is endemic in many bean-producing countries. Although Cff is a quarantine pathogen for Europe (EPPO A2 list), so far the control of imported seeds for Cff is not mandatory, and inspections often consist just on visual examination. Recently, the isolation of Cff from diseased soybean in Germany dramatically focused on the threat posed by its fur-ther spread. Therefore, it is urgently needed to implement rapid, easy and specific tests for Cff, having the potential to be routinely used at the ports of entry. A loop-mediated isothermal amplifica-tion (LAMP) assay for Cff was developed, targeting a nucleotide sequence exclusive of Cff strains pathogenic on bean. This LAMP test has a sensitivity of 10 pg/reaction, and the entire procedure takes only about 1.5 h.We gratefully acknowledge Ente Cassa di Risparmio di Firenze (project 2015.0999) and University of Florence (PIA 2017-2018) for the financial support.ORGANIC FARMING PROMOTES THE DEVELOPMENT OF BENEFICIAL SOIL MICROBIOTA THAT INCREASES RHIZOCTONIA SOLANI DISEASE SUPPRESSION. G. Bona-nomi1, G. Cesarano1, V. Antignani2, C. Di Maio1, F. De Filippis1, F. Scala1. 1 Department of Agricultural Sciences, University of Naples Federico II, via Università 100, 80055 Portici (Naples), Italy. 2 Depart-ment of Biology, Division of Natural Science, Bob Jones University, Greenville, USA, 29614. E-mail: giuliano.bonanomi@unina.itIntensive farming in agriculture raises serious concerns about the environmental sustainability due to the widespread use of JPP 99 (supp.).indb   41 22/09/17   10:06

LOOP-MEDIATED ISOTHERMAL AMPLIFICATION ASSAY FOR THE RAPID DETECTION OF CURTOBACTERIUM FLACCUMFACIENS pv. FLACCUMFACIENS FOR QUARANTINE AND FIELD APPLICATIONS

https://flore.unifi.it/bitstream/2158/1101577/1/SIPAV2017_JPP_Biancalanietal_S41.pdf

LOOP-MEDIATED ISOTHERMAL AMPLIFICATION ASSAY FOR THE RAPID DETECTION OF CURTOBACTERIUM FLACCUMFACIENS pv. FLACCUMFACIENS FOR QUARANTINE AND FIELD APPLICATIONS

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