Biricolti, Stefano

Bogani, Patrizia

Bramanti, E.

Campanella, B.

Cerboneschi, Matteo

Lanzini, G.

Macconi, S.

Moncelli, Maria Rosa

Onor, M.

Tadini Buoninsegni, Francesco

Tegli, Stefania

English

Florence Research

                                                    Società Italiana di Patologia Vegetale (S.I.Pa.V)           Dip.to Scienze Agrarie, Alimentari                         UNIVERSITA’ DI PISA Italian Phytopathological Society                                            ed Agro- ambientali ________________________________________________________________________________________________________________________ ___________________________________________________________________________________________________________  Sede del Convegno Aula Magna Polo didattico delle Piagge Università di Pisa Viale Giacomo Matteotti, 3 Pisa  AN INTEGRATED MODEL SYSTEM TO INVESTIGATE THE EFFECT OF COPPER ON PLANT PATHOGEN INTERACTION M. Cerboneschi1, G. Lanzini2, S. Macconi1, M. Onor3, B. Campanella3, E. Bramanti3, M.R. Moncelli4, F. Tadini-Buoninsegni4, S. Biricolti1, P. Bogani2, S.Tegli1 1Dipartimento di Scienze delle Produzioni Agroalimentari e dell’Ambiente, Università degli Studi di Firenze, Laboratorio di Patologia Vegetale Molecolare, Via della Lastruccia 10, 50019 Sesto Fiorentino, Firenze, Italy 2Dipartimento di Biologia, Università degli Studi di Firenze, Via Madonna del Piano 6, 50019 Sesto Fiorentino,Firenze, Italy 3ICCOM CNR, Via G. Moruzzi 1, 56124 Pisa, Italy 4Dipartimento di Chimica, Università degli Studi di Firenze, Via della Lastruccia 13, 50019 Sesto Fiorentino,Firenze, Italy E-mail:stefania.tegli@unifi.it  Copper is an essential trace element for any living organism and microrganism. However, copper is highly toxic when in excess, affecting several physiological and biochemical processes. Copper accumulates in the soil and the environment, so that a dramatic increase of free Cu2+ occurs in the agrosystems due to intensive copper applications as fungicides and bactericides. Additionally, its extensive use leads to the selection of copper-resistant bacteria, reducing the efficacy of copper treatments. Actually, copper tolerance was recently associated to the ability of several pathogenic bacteria for humans to survive the innate immune response during infection (Hodgkinson & Petris, 2012). Moreover, mutations in genes determining copper-resistance in the phytopathogenic bacterium Xanthomonas campestris pv. campestris resulted in the attenuation of its virulence (Hsiao et al., 2011). According to this unexpected role for copper in pathogenic bacteria – host interactions, the aim of our work was to develop an in vitro model to contribute to this new field of biometals research, based on Nicotiana benthamiana and N. tabacum challenged by Pseudomonas syringae pv. tabaci ATCC11528. By an integrated approach involving plant tissue culture, plant pathology, chemistry and electrochemistry, we provided evidences concerning the differential thresholds of copper toxicity on whole plant, plant pathogenic bacteria and even at subcellular level. On bacteria, copper toxicity was inversely correlated with the presence/availability of organic substances in the medium, suggesting a similar phenomenon occurring in soil. Moreover, copper damages were more pronounced on the host than on phytopathogenic bacteria, even at concentrations traditionally used in plant protection.   References Hodgkinson & Petris (2012) J. Biol. Chem. 287:13549-13555 Hsiao et al., (2011) J. Agric.Food Chem. 59: 9290–9302   Acknowledgments Project funded by EU, LIFE12 ENV/IT/336 "AFTER Cu" 

AN INTEGRATED MODEL SYSTEM TO INVESTIGATE

THE EFFECT OF COPPER ON PLANT PATHOGEN INTERACTION.

https://flore.unifi.it/bitstream/2158/957807/1/Tegli_SIPAV14.pdf

AN INTEGRATED MODEL SYSTEM TO INVESTIGATE THE EFFECT OF COPPER ON PLANT PATHOGEN INTERACTION.

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