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20th Int. Symp. “Animal Science Days”, Kranjska gora, Slovenia, Sept. 19th−21st, 2012.Acta argiculturae Slovenica, Supplement 3, 215–219, Ljubljana 2012COBISS: 1.08Agris category code: Q01 THE EFFECT OF GREEN HAM QUALITY ON THE TECHNOLOGICAL, CHEMICAL AND SENSORIAL TRAITS OF DRY-CURED HAM KRAŠKI PRŠUTCarolina PUGLIESE 1, Martin ŠKRLEP 2, Marjeta ČANDEK-POTOKAR 3 1 Dipartimento di Biotecnologie Agrarie, Via delle Cascine, 5 50144 Firenze, Italy, e-mail: carolina.pugliese@unifi.it2 Agricultural Institute of Slovenia, Hacquetova ul. 17, 1000 Ljubljana, Slovenia, e-mail: martin.skrlep@kis.si3 Same address as 2, e-mail: meta.candek-potokar@kis.siABSTRACTThis work studies the relationship among raw material characteristics, such as green ham pH value, fat thickness, trimmed ham weight and the main technological, chemical and sensorial traits, in the production of dry-cured Kraški pršut. For the scope of the study a sample of 251 hams, supplied by two producers, was used. Trimmed ham weight, fat thickness and pH value of semimembranosus muscle (pH24) were recorded on green hams. On dry cured hams the following parameters were assessed: colour (CIE L*,a*,b*), intramuscular fat (IMF), proteolysis index, salt percentage, sensorial analysis. Principal component analysis (PCA) was used to assess the relationships among variables. Among green ham traits, pH24 affected the largest number of parameters measured on the dry-cured ham. Higher pH24 of green ham was associated with darker muscles of dry cured ham, lower salting and seasoning losses. Extreme values of pH24 were associated with the highest values of proteolysis index. As regards sensorial traits, harder and dryer texture was associated with the increase of pH24. Salt percentage was negatively associated with IMF. Fat thickness was negatively as-sociated with salting and seasoning losses. No significant effect of green ham weight was found on any of the parameters considered in this study. As regards PCA analysis the first two components explained 34.5% of the variance. In the first one, trimmed weight, salting and seasoning losses, moisture of biceps femoris and semimembranosus muscles showed the highest loading values. In the second one saltiness, texture, IMF content, showed the highest loading absolute values.Key words: green ham traits / dry-cured ham / Kraški pršut / technological quality / chemical composition / sen-sory traits1 INTRODUCTIONOne of the most appreciated dry-cured meat prod-ucts in Slovenia is dry-cured ham Kraški pršut, which is waiting for the final approval of European Commission for protected geographical indication (PGI). To ensure consistent quality and typical nutritional and sensory properties the producers can play according to several aspects of dry curing process such as the environmental conditions (temperature, relative humidity) and season-ing time. Some of these are reported in the PGI specifica-tion for Kraški pršut, however no indications are given in terms of characteristics of pigs that provide raw material. The protection of PGI does not consider a control over the origin of raw material, which however is a key factor in the case of dry-cured hams (for review see Čandek-Potokar and Škrlep, 2012). Once the process of drying is standardised the only factor affecting the quality of dry-cured ham is its raw material quality. Dry-curing is a very complex process which involves many biochemical reac-tions and changes. Induced changes concern water loss and salt intake affecting mainly proteins and lipids. Dur-ing seasoning proteins and lipids undergo an intense pro-teolysis and lipolysis, resulting in a great amount of small peptides, free amino acids, free fatty acids and a great number of volatile compounds characterising the prod-uct. Kraški pršut is now days produced with thighs be-longing to conventional pig production characterised by Acta agriculturae Slovenica, Supplement 3 – 2012216C. PUGLIESE et al.relatively low slaughter weights and high leanness which are generally associated with higher seasoning loss and lower dry-cured ham sensory quality (Guerrero et al., 1996). Studies on the eff ect of raw material on dry-cured ham quality have been carried out extensively on many Mediterranean types of product e.g. Iberian Parma, San Daniele, Bayonne. Such studies have been performed on Kraški pršut but in a limited extent (Čandek-Potokar et al., 2002; Čandek-Potokar and Škrlep 2011; Škrlep et al., 2011a; Škrlep et al., 2011b). In view of the restricted in-formation, the aim of this work was to study the relation-ship among green ham characteristics, such as pH24, fat thickness, trimmed weight and the main technological, chemical and sensorial traits, in dry-cured ham Kraški pršut. 2 MATERIAL AND METHODS For the present study 251 hams were used. Two pro-ducers of dry hams participated in the present study giv-ing twelve batches of hams in total. All the green hams were weighed before and aft er the trimming phase. Sub-cutaneous fat was measured on each trimmed ham below caput ossis femoris. Th e measurements of ultimate pH value (pH24) were taken in m.  semimembranosus (SM) with the pH Meter MP120 (Mettler-Toledo, GmbH, 8603 Schwarzenbach, Switzerland) on two sites within mus-cle: Weight of the hams was recorded aft er trimming, second salting and ripening. Processing losses (%) were calculated as cumulative losses at the end of each phase. On SM and biceps femoris (BF) moisture content was performed according to the ISO 6496 (ISO 6496, 1999). At the end of the seasoning process, the following deter-minations were carried out on SM and BF: 1) Minolta L*a*b* Chroma and Hue values were measured (Minolta Chroma Meter CR-300, Minolta Co, Osaka, Japan), 2) intramuscular fat content determined according to ISO 1443 (ISO, 1443, 2001) using the Büchi Extraction Sys-tem B-811 (Büchi Labortechnik AG, Flawil,Switzerland); 3) protein calculated from total nitrogen content and determined according to ISO 5983-2 (ISO 5983-2, 2005) using the Kjeltec 2300 nitrogen analyser (Foss Analytical, Hileroed, Denmark). Non-protein nitrogen content was determined as described in Monin et al. (2007) and ex-pressed as a percentage of total nitrogen (proteolysis in-dex); 4). Sodium chloride (salt) content was determined as described in Monin et al. (1997). On all seasoned hams sensory evaluation was carried out by six trained pan-ellists who assessed sensorial traits on a 7-point scale, with intensity increasing from 1 to 7. Th e analysis was performed in 18 diff erent sessions and the sample order was randomised. As regard statistical analysis, fi rstly the original data set of panel test was submitted to analysis of variance (GLM procedure SAS Inc., Cary, NC, USA) with fi xed eff ect of sensory session and residual values were taken. Data set with all variables was then submitted to analysis of variance (GLM procedure SAS Inc., Cary, NC, USA) including producer, batch, producer×batch inter-action as fi xed and pH24, fat thickness, trimmed weight as covariates. Th e corresponding regression equations were calculated. Principal component analysis (PCA) was ap-plied to evaluate the relationships among variables and latent variables were constructed.3 RESULTS AND DISCUSSIONIn the fi gures 1–5 the relationship among pH24 and the main quality traits of dry-cured ham are reported, in Table 1 the corresponding equations of regression are shown. As regard colour parameters, a decrease of L* value is shown according to the increase of pH24 (Fig. 1), even if this trend was signifi cant for SM muscle only. With the increase of pH24 a signifi cant decrease of Chroma and Hue values was also evident (Figures 2, 3), in particular for SM muscle. Th e SM muscle showed the highest correlation with pH24 because it was the mus-cle where the ultimate pH was recorded. Th e decrease of Hue value, mainly due to a signifi cant decrease of b* (data not shown), indicate the prevalence of a* param-eters and, consequently, of the red colour. As regards technological yields, apart from the strictly statistical signifi cance, a decrease of process-Figure 1: Evolution of L* Figure 2: Evolutiuon of chroma Figure 3: Evolution of hueActa agriculturae Slovenica, Supplement 3 – 2012 217THE EFFECT OF GREEN HAM QUALITY ON THE TECHNOLOGICAL, CHEMICAL ... DRY-CURED HAM KRAŠKI PRŠUTFigure 4: Evolution of proteolysis index Figure 5: Evolution of IMF Figure 6: Evolution of salt contentFigure 7: Evolution of texture Figure 8: Evolution of salting loss Figure 9: Evolution of seasoning lossing losses according to the increase of pH24 was found, particularly during salting phase (data not shown). Th is confi rms previous results on Kraški pršut, where lower losses at higher pH24 were recorded, in particular in the fi rst salting phase, (Čandek-Potokar and Škrlep, 2011), as well as on other type of dry cured ham (Schivazappa et al., 2002).In Figure 4 the proteolysis index trend is reported. At the extreme values of pH24 the highest values of proteoly-sis indexes were found. As reviewed by Čandek-Potokar and Škrlep (2012) a lower pH causes the breakdown of lysosomes and the subsequent release of proteolytic en-zymes into the muscular tissue, the enhancement of the activity of certain enzymes (cathepsins), which can lead to excessive proteolysis and pastiness problems. Higher pH can also cause the release of lower amounts of water, less intake of salt and, subsequently, pastiness and soft -ness due to higher water activity (aw) and less inhibition of enzymatic activity. In regard to IMF a signifi cant trend according to Figure 10: Loading plot of the main quality traitsActa agriculturae Slovenica, Supplement 3 – 2012218C. PUGLIESE et al.variation of pH24 was found on SM muscle (Fig. 5). IMF increased with the increase of the pH24, at least within the range of values considered normal for this parameter. Probably, this trend is not related directly to pH24 but to the variation in salt percentage (affected by pH), in par-ticular on SM muscle (Fig. 6). Similar results were found by Ruiz-Ramírez et al. (2005) in a study on the effect of different level oh pH24 on dry-cured traits. They also ex-plained the inverse relationship between salt and IMF percentage according to differences between tissues, in diffusivity of NaCl that is eight times lower in subcutane-ous fat than in lean meat. In Figure 7 the relationship between texture and pH24 values is reported. Texture (higher score denotes harder and drier texture) was the only sensorial param-eter significantly affected by pH24. In both muscles it was positively correlated to pH value. This result is in con-trast with what is known about the relationship between the two parameters (at least in fresh meat), namely that lower meat pH, closer to the isoelectric point of myosin, increases intermolecular linkages between negatively and positively charged groups (Hamm, 1986) and thus hardness. It is probable that the trend of texture values found in this study is related to salt content evolution (both were very similar). In fact, as reported in the above mentioned study (Ruiz-Ramírez et al., 2005) at high NaCl concentration, the myofibrillar structure gets more compact, along with an inhibitory effect of NaCl on the enzyme activities. As regards the effect of fat thickness, it significantly affected salting and seasoning losses (Fig. 8, 9) which, as expected, decreased with an increase of fat thickness. As reported by Bosi and Russo (2004) a sufficient subcutane-ous fat layer is necessary to prevent rapid desiccation and reduction of processing losses. Fat thickness significantly affected only two sensorial traits: texture and saltiness. The former decreased, in particular in BF, according to the increase of fatness. Fat thickness and texture are thus indirectly correlated, due to the positive correlation be-tween texture and intramuscular fat (Ruiz-Carrascal et al., 2000) the latest being positively related to fat thick-ness (Bahelka et al., 2007). As regard saltiness it was only slightly affected by fat thickness in the case of SM, dem-onstrating lower saltiness with increasing fatness. No significant effect of green ham weight was found for any of the parameters considered in this study. This Significance of linear regression(X)*Significance of quadraticregression(X2)*Regressions on pH24L*BF = 16.9411736 + 8.82053986 * X − 0.79515801 * X2 ns nsL* SM = −76.6303595 + 38.7964862 * X − 3.37656203 * X2 * *Chroma BF = −16.134685 + 11.00971912 * X − 0.96984462 * X2 ns nsChroma SM = −68.824103 + 28.22346661 * X − 2.41935589 * X2 * *Hue BF = −2.31661817 + 0.933698715 * X − 0.080406246 * X2 * *Hue SM = −5.51165138 + 2.085860918 * X − 0.175583943 * X2 * *IP BF = 121.175309 − 30.4875067 * X + 2.3964874 * X2 ns nsIP SM = 148.410825 − 41.6389578 * X + 3.2841524 * X2 * *IMF % BF = −13.737773 + 5.30853305 * X − 0.44008552 * X2 ns nsIMF % SM = −35.2581497 + 12.23211065 * X − 0.99475766 * X2 * *Salt % BF = 23.3555643 − 5.34031822 * X + 0.45946637 * X2 ns nsSalt % SM = 40.617222 − 11.28908376 * X + 0.93159937 * X2 * *Texture BF = 23.5397297 − 6.54899837 * X + 0.53284923 * X2 * *Texture SM = 20.3760557 − 5.28923335 * X + 0.43697675 * X2 * *Regressions on fat thickness Salting loss % = 6.91562413 − 0.189775568 * X + 0.002269871 * X2 * *Total loss % = 43.9943279 − 0.61501735 * X + 0.00699108 * X2 * *Table 1: Equations of regression* the regression coefficients were significant for P < 0.05.Acta agriculturae Slovenica, Supplement 3 – 2012 219THE EFFECT OF GREEN HAM QUALITY ON THE TECHNOLOGICAL, CHEMICAL ... DRY-CURED HAM KRAŠKI PRŠUTcan be explained by the fact that the effect of weight on the qualitative characteristics of dry-cured ham is basi-cally included in the effect of adiposity.In the loading plot generated by the PCA analysis (Fig. 10), the differences and the similarity between traits are reported. The first component, that explained 20.7% of variance, could be considered as “technological latent variable”, where parameters such as trimmed weight, salt-ing and seasoning losses, moisture of BF and SM, showed the highest loading values. The second component, that explained the 13.83% of variance, could be considered as “sensorial latent variable” where, on both muscles, salti-ness, texture, IMF content showed the highest loading absolute values. As regard the relationship among vari-ables, almost all the technological variables are placed in the second quadrant at the opposite of those related to salt and saltiness placed in the fourth quadrant, which testifies the inverse relationship among them. Finally, in the third quadrant, the adiposity variables are placed. The low relationship observed between BF and SM dem-onstrates important differences between the two muscles. 4 REFERENCES Bahelka I., Hanusová E., Peškovičová D, Demo P. 2007. The ef-fect of sex and slaughter weight on intramuscular fat con-tent and its relationship to carcass traits of pigs. Czech Jour-nal of Animal Science, 52: 122–129Bosi P., Russo V. 2004. The production of the heavy pig for high quality processed products. Italian Journal of Animal Sci-ence, 4: 309–321Čandek-Potokar M., Škrlep M. 2012. Factors in pig production that impact the quality of dry-cured ham: a review. Animal, 6: 327–338Čandek-Potokar M., Škrlep M. 2011. 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Meat Science, 47: 29−47Ruiz-Carrascal J., Ventanas J., Cava R., Andrés A.I., García C. 2000. Texture and appearance of dry cured ham as affected by fat content and fatty acid composition. Food Research International, 33: 91–95Ruiz-Ramírez J., Arnau J., Serra X., Gou P. 2005. Relationship between water content, NaCl content, pH and texture pa-rameters in dry-cured muscles. Meat Science, 70: 579–587Schivazappa C., Degni M., Nanni Costa L., Russo V., Buttazzoni L., Virgili R. 2002. Analysis of raw meat to predict proteoly-sis in Parma ham. Meat Science, 60: 77–83Škrlep M., Čandek-Potokar M., Mandelc S, Javornik B., Gou P., Chambon C., Sante-Lhoutellier V. 2011a. Proteomic profile of dry-cured ham relative to PRKAG3 or CAST genotype, level of salt and pastiness. Meat Science, 88: 657–667Škrlep M, Čandek-Potoka, M, Sante-Lhoutellier V., Gou P. 2011b. Dry-cured ham ‘Kraški pršut’ seasoning losses as af-fected by PRKAG3 and CAST polymorphisms. Italian Jour-nal of Animal Science, 10: 27–32

The effect of green ham quality on the technological, chemical and sensorial traits of dry-cured ham Kra&#353;ki pr&#353;ut

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The effect of green ham quality on the technological, chemical and sensorial traits of dry-cured ham Kraški pršut

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The effect of green ham quality on the technological, chemical and sensorial traits of dry-cured ham Kra&#353;ki pr&#353;ut

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Florence Research

The effect of green ham quality on the technological, chemical and sensorial traits of dry-cured ham Kraški pršut