Betti, G.

Cervellin, F.

Giannelli, R.

Labrada Ching, J.

Lorenzini, Giangiacomo

Martini, Andrea

Pieri, S.

Riccio, F.

English

Florence Research

AFLATOXINS ON MILK (ORGANIC AND BIODINAMIC) MARKETED IN FLORENCE AREA  Martini, A1., Lorenzini, G1., Labrada Ching J.2, Riccio, F1.,  Cervelin, F3., Betti, G3., Giannelli, R3., Pieri, S3.  1 Dipartimento di Scienze Zootecniche University of  Florence; Dipartimento di Scienze Zootecniche Università degli Studi di Firenze via delle Cascine, 5 50144 Firenze – Italia tel. +39/0553288357 fax +39/055321216 andrea.martini@unifi.it 2 Facultad de Ciencias Agropecuarias, Universidad de Camagüey (Cuba) 3 Centrale del Latte di FI, PT e LI “Mukki Latte”   Introduction  It is believed that risks linked to aflatoxins (AFM1) contamination are higher in organic milk production than in conventional.  In a past study (Lorenzini et al., 2004) we demonstrated that the AFM1 is not only a problem for organic agriculture, but also for conventional agriculture. Therefore, adopting preventive measures, it is possible to produce safe milk for human consumption also in Mediterranean areas where the warmer climate increases the risk of AFM1 contamination in the maize.  The aim of this work has been to establish what is the actual risk linked to AFM1 contamination of milk in the organic Italian market.  Methodology  The study was initiated with a literature review, in order to evaluate the problem of the AFM1 in the milk marketed in the Florence area.  Dairy Centre of Florence, Pistoia and Livorno (Mukki Latte) carried out the laboratory analyses on organic and biodynamic milk, using the official methodology (HPLC).  The analyses were carried out for three times, every ten days, on samples bought in the supermarkets of Florence throughout February 2005.  Table 1 Organic milk samples.   Organic Biodynamic  Pasteurized UHT UHT  Entire Partially skimmed Entire Partially skimmed Entire Partially skimmed N° of samples 4 2 3 1 1 1         Amongst the methodologies used for the analysis of AFM1, the liquid chromatography was the most efficient. This methodology has a high sensitivity and specificity and with this it is possible to resolve 5 ppt (ng/kg) of AFM1 in milk. In comparison to the HPLC method, the ELISA (Enzyme-Linked Immunosorbent Assay) methodology shows the following advantages: high specificity, relatively simple and short assay time (4 hours).  The data obtained from the analyses were reported on tables underlining the legal limits.  Results Literature review   One study was carried out on 2002 to evaluate the occurrence of AFM1 in milk and dairy products (Capei and Neri, 2002). A total of sixty samples of commercial milk were collected in supermarkets in Florence and checked for AFM1 by using direct competitive enzyme-linked immunosorbent assay (ELISA). The incidence of AFM1 contamination in samples analysed was very high (91-94% of positive samples). It was found, above detection limit (d.l.) of 3 ppt, in 55 (91.6%) of the milk samples in amounts ranging from 3 to 35 ppt with a mean concentration of 9.3 ppt. None of milk samples exceeded the European and Italian legal limit of 50 ppt. The results showed a diffuse microcontamination of AFM1 in samples analysed, which does not appear to be a serious risk to public health.   Table 2 Market trend of milk (Data referred 2001 and % variations on the previous year).  Product Market in volume (tons) Dynamics of     consumptions (%) Pasteurized milk 449.000 + 5,7 UHT milk 896.000 + 4,3  Table 3 Occurrence and levels of AFM1 in milk.   Number of samples  Pasteurized milk UHT milk  N. (%) N. (%) < d.l. 1    (6) 4     (9) > d.l. 16  (94) 39   (91) Total 17 43 Range (ppt) N.  (%) N.  (%)  3 - 10  6    (38) 30   (77) 11 – 20 6    (38) 6   (15) 21 – 30 2    (13) 2    (5) 31 – 40 2    (13) 1    (3) 41 – 50 0 0 media ± S.D. 15,5 ± 9,8 7.5 ± 7,2 d.l. = detection limit (3 ppt)  Figure 1 AFM1 dispersion in milk samples.   Analyses made by Mukki Latte  Mukki Latte commercializes organic milk under the label “Podere Centrale” from two dairy farms in Mugello and one from Lazio (pasteurized entire and partially skimmed, and UHT entire). Mukki Latte carried out analyses on organic milk, using the official methodology HPLC.  We can evaluate that the organic milk marketed in Florence reach about the 5-6%, and the Podere Centrale label represents the 80% of this amount.  Table 4 AFM1 in organic milk. Number of samples analysed by Mukki Latte.   Organic Biodynamic  Pasteurized UHT UHT  Entire Partially skimmed Entire Partially skimmed Entire Partially skimmed 1st analysis       < d.l. 2 1 3 1 1 - > d.l. 2 (7 and 24 ppt) 1 (13 ppt) 0 0 0 - 2nd analysis        < d.l. 0 1 3 1 - 1 > d.l. 2 (7 ppt both) 1 (14 ppt) 1 (14 ppt) 0 - 0 3rd analysis       < d.l.     - - > d.l.     - - d.l. = detection limit (5 ppt)  It was impossible to find all the kinds of milk for all the three analyses, because some milk labels were not available in the supermarkets.  Conclusions   1) The Biodynamic milk seems always free for AFM1. But, for the biodynamic philosophy, a milk sold as UHT could be not so correct. 2) Few samples (pasteurized and UHT) showed positive results for AFM1, but all under the EU legal limits of 50 ppt. 3) Comparing these results with the bibliographic data about conventional milk market in Florence area in 2001, we can assert that the risk of AFM1 in organic milk is at a very low level. 4) May be in the future, using the adopting preventive measures, it will be possible to produce organic milk without AFM1. 5) Possible solutions to realise this aim are the following: a. Preventive analysis on milk and feed produced on the organic farms, b. Adopting a good management of the maize crops, c. Adopting a correct harvesting and keeping maize system.   References  Lorenzini, G., Martini, A., Contini, C., Omodei Zorini, L., Riccio, F., Cervelin, F., Betti, G., Giannelli, R., Casini, M., (2004) Mycotoxins in the milk from organic farms in the Florence province, Proceedings of the 3rd SAFO Workshop, Falenty, Poland. 16-18 September 2004: 69-82.  Capei, R., Neri, P., (2002) Occurrence of aflatoxin M1 in milk and yoghurt offered for sale in Florence (Italy), Ann. Ig. 2002 Jul-Aug; 14 (4): 313-9.  

AFLATOXINS ON MILK (ORGANIC AND BIODYNAMIC) MARKETED IN FLORENCE AREA

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AFLATOXINS ON MILK (ORGANIC AND BIODYNAMIC) MARKETED IN FLORENCE AREA

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