Jin, Hong

Yun, Lan

Zhang, Yongming

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Population Genetic Diversity of Potaninia mongolica Maxim Hong Jin Inner Mongolia Agricultural University, China Yongming Zhang Inner Mongolia Normal University, China Lan Yun Inner Mongolia Agricultural University, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/20-1/16 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 ]1012　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands People and Policies ——— Institutional Innovations for the Conservation of Grasslands/Rangelands BiodiversityPopulation genetic diversity of Potaninia mongolica MaximJin Hong1 Zhang Yong‐ming2 ,Y un lan11 College o f Ecological and Env ironmental science in Inner Mongolia A gricultural University , Hohhot , Inner Mongolia010019 ,China . 2 College o f L i f e Science and Technology in Inner Mongolia Normal University , Hohhot ,Inner Mongolia010022 ,China ; E‐mail :j inhong915＠ 163 .comKey words : Potaninia mongolica Maxim ,population ;genetic diversity ,AFLPIntroduction Research of genetic diversity provides important informationfor analyzing why some species become rare andendangered , and it provides information that can be used for protecting a species .( Greef et al ,１９９７ ) . We used AmplifiedFragment Length Polymorphism( AFLP) to study Potaninia mongolica Maxim in different regions , in order to explore thegenetic background and evolutional processes of these populations .Materials and methods In the present paper , we studied the genetic diversities of eight wild P . mongolica Maxim . Populations( No .１ — No .８ ) , selected on the Western Ordos and Eastern Alax plateau . We collected thirty random samples in eachpopulation and extracted the total DNA in the lab . PCR selective amplifications were performed with eight primer combinationsof ２ EcoR I and ４Mse I .The AFLP data were analyzed with PopGen３２ .Results Genetic diversity and differentiation among eight populations ( ２４０samples ) were studied using AFLP analysis with ８informative primer pairs . ３９７ unambiguous bands( Figure １) were created between ６５‐５３０bp , ２８６ of which were polymorphic .One pair of AFLP primers has detected a mean of ３５ .７５ . The result indicated relatively high level of genetic variation in thisspecies . according to the genetic distance matrix shown in attached Table １ . It is shown by the gendrogram ( Figure ２) that the８ populations can be categoried into ４ groups . a low degree of population differentiation existed among the eight populationswith GST values of ０ .１３ .Figure 1 A FL P f ingerp rinting o f P . MongolicaMaxim . M : MakerFiguer 2 Dendrogram based on Nei摧s geneticdistance o f all P . Mongolica Max im . populationsDiscussion The capabilities of evolutionalpotential and resisting severe environmentsrests with variation and genetic structure ofpopulations ( Arise & Hamrick , １９９６ ) . Thegenetic structure of P . Mongolica Maximreflected its evolutional history of populationand determined adaptive potential for thefuture . Genetic diversity of P . MongolicaMaxim . populations shows clear regionalrelativity and similar genetic style , it showsthey have a common origin . The randomgenetic vagrancy and inbreeding declinearen摧t affecting genetic diversity .Table 1 Nei摧s genetic identity o f all P .Mongolica Maxim . populationsPopulation １ @２ d３ 垐４ �５ 行６ 趑７  ８ R１ 行‐２ 行０ �.１３ ‐３ 行０ �.１２ ０  .１４ ‐４ 行０ �.１３ ０  .１２ ０ C.１７ ‐５ 行０ �.１３ ０  .１４ ０ C.１９ ０ g.２０ ‐６ 行０ �.１１ ０  .１２ ０ C.１４ ０ g.１４ ０ 媼.１４ ‐７ 行０ �.０９ ０  .１２ ０ C.１２ ０ g.１３ ０ 媼.１３ ０ .０８ ‐８ 行０ �.１３ ０  .１２ ０ C.１４ ０ g.０７ ０ 媼.１６ ０ .１４ ０ 佑.１５ ‐Average ０ �.１３ReferencesGreef J M , Deuter M , Jung C , Scohondelmaier J .(１９９７ ) .Genetic diversity of European Miscanthus species revealed by AFLPfingerprinting . Genetic Resources and Crop Evolution . ４４ : ２ , １８５‐１９５ .Arise J .C . and Hamrick J .L . (１９９６) . Conservation genetics , case histories from nature . Chapman & Hall . New York . １８９‐１９０ .

Population Genetic Diversity of \u3ci\u3ePotaninia mongolica\u3c/i\u3e Maxim

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3871&amp;context=igc

Population Genetic Diversity of \u3ci\u3ePotaninia mongolica\u3c/i\u3e Maxim

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