Gao, Weihua

Han, Jie

Wang, Hongyan

Zhi, Zhongsheng

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Analysis of Peroxidase Isozymes in Eight Populus Cultivars Weihua Gao Inner Mongolia Agricultural University, China Jie Han Inner Mongolia Forestry Science Academy, China Zhongsheng Zhi Inner Mongolia Agricultural University, China Hongyan Wang Inner Mongolia Agricultural University, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/15-3/23 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 770　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems ——— Forage Quality ,Conservation and UtilizationAnalysis of peroxidase isozymes in eight Populus cultivarsGao Weihua1 Han Jie2 Zhi Zhongsheng 1 Wang Hongyan11 Inner Mongolia Agriculture University , Hohhot 010019 ,China ; 2 Inner Mongolia f orestry Science Academy , Hohht010010 ,ChinaKey words :Populus ,peroxidase isozymes ,polyacrylamide gel electrophoresis ,hereditary identification ,cluster analysisIntroduction In recent years isozymes have been widely used for classification and hereditary analysis of plants such as crops ,vegetables ,herbs , and trees . It is a simple method with high sensitivity and reproducibility ; results are easy to observe andrecord . This technique has been adopted to analyse peroxidase isozyme patterns in eight Populus cultivars using polyacrylamidegel electrophoresis . The analysis of isozyme patterns and their hereditary differences provides the basis for developing newvarieties of Populus .Material and methods Plant material was collected from the botanical garden of the Inner Mongolia Forestry Science ResearchAcademy ( Table １ ) . Polyacrylamide gel electrophoresis has been used to separate isozymes .　 Figure 1 Dendrogram o f cluster analysis based on isoz yme data f or Populus germplasm .Table 1 Plant material and their origin .code cultivars original１ Eur‐America１０７ Beijing Forestry Department２ Zhonglin ４６ China Forestry Science Academy３ １７２ China Forestry Science Academy４ ８８５８‐６４ China Forestry Science Academy５ Qun zhongyang Inner Mongolia Forestry Science Academy６ １５３ Inner Mongolia Forestry Science Academy７ Faku １ Tongliao , Inner Mongolia８ Zhelin ４ Tongliao , Inner MongoliaResults analysis According to the POD isozyme patterns , the eight cultivars were divided into two main clusters : of the sevenperoxidase isozymes present in Populus , the second , third , and sixth band was present in zhonglin ４６ ,１７２ ,１０７ and ８８５８‐６４ ,which were clustered into cluster one . The other four cultivars , Qun zhongyang , Zhelin ４ ,１５３ , and Faku １ lacked the secondand the third band but had the fourth band , were clustered into cluster two .In cluster one Zhonglin １７２ and ８８５８‐６４ clustered closely together , whereas Eur‐America １０７ and Zhonglin ４６ were fartherapart . In cluster two Qun zhongyang and Faku １ , and １５３ and Zhelin ４ , respectively , clustered closely together .Conclusions POD isozyme analysis of Populus shows the presence of up to seven different bands ( isoforms) . The difference inisozyme patterns allows the classification of Populus cultivars into distinct clusters . Cluster analysis gives information on therelationship between cultivars and can be used for hereditary classification .

Analysis of Peroxidase Isozymes in Eight \u3ci\u3ePopulus\u3c/i\u3e Cultivars

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Analysis of Peroxidase Isozymes in Eight \u3ci\u3ePopulus\u3c/i\u3e Cultivars

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