Su, Yanjing

Sun, L. S.

Yan, C. J.

Zhao, Guoqi

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Analysis of Cell Wall Components and Ultra‐Structure for BM‐Rice Animal Nutrition Purposes Yanjing Su Yangzhou University, China C. J. Yan Yangzhou University, China L. S. Sun Yangzhou University, China Guoqi Zhao Yangzhou University, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/15-3/12 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　 瞯 759　 瞯Grasslands/Rangelands Production Systems ——— Forage Quality ,Conservation and UtilizationAnalysis of cell wall components and Ultra‐structure for BM‐Rice animal nutrition purposesY .J .Su1 , C . J . Yan2 , L . S . Sun1 , G . Q . Zhao11 College o f Animal Science and Technology , Yangzhou University , Yangzhou 225009 , China , E‐mail : j szhaoguoqi ＠ sohu .com , 2 College o f Agriculture , Yangzhou University , Yangzhou 225009 , ChinaKey words : brittleness mutation rice ,Cell wall components ,Ultra‐structureIntroduction Brittleness mutation ( BM ) rice , selected though ６０ Co ( gamma ray ) , is a kind of mutated rice from j aponicavariety Zhonghua‐１１ with the characters of softness , greenness and fragility . This mutant was expected to change contents offiber component and the linking structure of cell wall for the rice straw on the genetic basis , so as to cultivate a new kind offodder with high digestibility . This essay aims at providing certain theoretical basis for studying the degradation efficiency ofBM‐rice straw by contrasting and analyzing the difference of contents of fiber component and ultra‐structure between BM‐riceand its wild type .Materials and method Analysis for cellulose , hemi‐cellulose , ADL ( Acid detergent lignin) and AIA ( Acid insoluble ash ) werecarried out according to principles outlined by Van Soest (１９８５ ) . All tissues were stored at ４ ℃ for approximately one monthbefore post‐fixation in １ .５ ％ OsO４ . After several rinses , the material was dehydrated in an ethanol series and then embedded inlow viscosity resin . Thin sections (６０ to １００nm) were cut with a diamond knife , collected on copper grids and stained with １ ％aqueous uranylacetate for １５ min followed by １５min in Reynold摧s lead citrate ( Kornfeld A et al . , ２００７ ) . The sections wereexamined in a transmission electron microscope ( TEM . Phillips Tecnai １２ . Holland) .Samples taken from the fixative were cutwith a razor blade to expose a gland for scanning electron microscope ( SEM ) analysis . The tissue was post‐fixed in OsO４ ,dehydrated as above ; critical point dried in CO２ , spurted with gold and examined in a scanning electron microscope ( GoldsteinJ I et al . , １９９２ ) ( SEM . PHILIPS XL‐３０E . Holland)Results (１ ) Compared with its wild type , the cellulose content of BM‐rice leaf , stem and root was lower ( P ＜ ０ .０１ ) (１８ .９９ ％ ,３７ .２９ ％ and ２３ .３４ ％ , respectively) , and hemi‐cellulose content of BM‐rice leaf , stem and root higher ( P ＜ ０ .０１ ) (４６ .０７ ％ ,８５ .２６ ％ and ６１ .９６ ％ , respectively ) . ( ２ ) Through the observation by Scanning electron microscope , there were largedifferences in tissue between BM‐rice and its wild type . In BM‐rice leaf tissue , parenchyma was enlarged , sclerenchyma wasshrunken and the number of vascular bundles was reduced . In outer epidermis of BM‐rice stem , distribution of strumae wassparse . In duramen of BM‐rice root , the link of phloem was loosed and dispersed . ( ３ ) Through the observation byTransmission electron microscope , there were large differences in cell morphology and cell wall structure for BM‐rice . Insclerenchyma cell of BM‐rice leaf , the hiberarchy of cell wall was not obvious . In parenchyma of BM‐rice stem , the sizes ofcells were diverse and the arrangement of cells was disordered . In parenchyma cell of BM‐rice root , the exterior of cell wall wasknaggy .Conclusions Based on the analysis of Cell Wall Components and Ultra‐structure for BM‐Rice , it can be said that BM‐rice havepotential advantage in its utilization . This advantage not only plays a vital role in enhancing the straw摧s utilization , but also besurely benefit to cow breeding industry摧s development in south china .ReferencesKornfeld A , Kaufman P .B . , Lu C .R . , et al . , ２００７ . The Production of hypericins in two selected Hypericum perforatumshoot cultures is related to differences in black gland structure . Plant physiology and Biochemistry , ４５ :２４‐３２ .Goldstein J .I . , Newbury D .E . , Echlin P . , et al . , １９９２ . Scanning Electron Microscope and X‐ray Microanalysis , secondedition . New York : Plenum Press . ８１７‐８２１ .

Analysis of Cell Wall Components and Ultra‐Structure for BM‐Rice Animal Nutrition Purposes

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3714&context=igc

Analysis of Cell Wall Components and Ultra‐Structure for BM‐Rice Animal Nutrition Purposes

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