Chen, Tuoxiong

Hao, Wenjun

Lu, Xinshi

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Influence of Salinity on Seed Germination of 10 Alfalfa Varieties Tuoxiong Chen Beijing Forestry University, China Wenjun Hao Bohai University, China Xinshi Lu Beijing Forestry University, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/14-2/32 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 ]630　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems——— Seed Science and TechnologyInfluence of salinity on seed germination of 10 alfalfa varietiesTuox iong CHEN 1 , Wenjun HAO2 , X inshi LU11 Forestry College , Bei j ing Forestry University , 100083 China ; 2 College o f Tourism , Bohai University , 121000 China ;chentx03＠ lz u .cnKey words : alfalfa ,Medicago sativ a ,salinity ,germination rateIntroduction It is estimated that salinity affects at least ２０％ of world摧s arable land and brings more than ４０％ irrigated land tovarious degrees( Rhoades and Loveday ,１９９０) . Alfalfa(Medicago sativa L .) is moderately sensitive to salinity and is considered asthe most important forage crop . Germination is very important to the plant establishment in saline‐soil region . The present investigationwas aimed to study the effect of NaCl salinity on alfalfa germination .Materials and methods Seeds of １０ alfalfa varieties were sterilized with ０ .５％ sodium hypochlorite solution for one minute andthen washed twice with distilled water . Seeds were germinated on ２ sheets of ９ cm diameter filter paper in １０ cm diameterPetri‐dishes at concentrations of ０ ,５０ ,１００ ,１５０mM NaCl with １０ ml solution . Each treatment incuded three replications , andfif ty seeds were used for each replication . Germination was recorded at ２４h intervals . After １０ days , ungerminated seeds weretransferred to distilled water for ９ days to determine the recovery of germination .Results and discussion The experimental results indicate that the difference of the germination rate for alfalfa varieties in thesame NaCl concertration is significant and that the difference of germination rate for the same variety in the different NaCl isalso significant . In general , salinity inhibited seed germination and increasing NaCl concentration significantly reducedgermination rates( Table １) . The percentage of recovery from high salinity concentrations was higher than low concentrations .Under the treatments with １００ ,１５０ mM NaCl solution , the percentages of germination and those after being transferred todistilled water were lower than the none‐saline control , indicating that parts of the NaCl treated seeds have permanently losttheir germination ability( Table ２ ) .Table 1 Germination rate .VarietyDifferent concentration in the same variety Different variety in the same concentrationNaCl concentration NaCl concentration０ mM ５０ mM １００ mM １５０ mM ０ mM ５０ mM １００ mM １５０ mMMaverick ７６ !.６７a ４２ /.００b ２２ =.６７b ０  .６７c ７６ Y.６７def ４２ g.００e ２２ t.６７de ０ T.６７eVernal ８７ !.３３a ７６ /.６７a １８ =.００b ０  .６７b ８７ Y.３３bcd ７６ g.６７bc １８ t.００e ０ T.６７ePioneer５４４６ m９５ !.３３a ８７ /.３３ab ７６ =.００b ４  .６７c ９５ Y.３３a ８７ g.３３ab ７６ t.００a ４ T.６７cdeLegend ６２ !.６７a ５０ /.６７ab ３０ =.６７b ０  .６７c ６２ Y.６７f ５０ g.６７de ３０ t.６７cde ０ T.６７eArcher ６７ !.３３a ７１ /.３３a ４０ =.００b ２  .６７c ６７ Y.３３ef ７１ g.３３bc ４０ t.００bcde ２ T.６７deABI７００ 技９２ !.６７a ８４ /.６７a ４５ =.３３b ２  .６７c ９２ Y.６７abc ８４ g.６７ab ４５ t.３３bcd ２ T.６７deDona Aan ９５ !.３３a ９５ /.３３a ７２ =.６７b ２４ K.６７c ９５ Y.３３ab ９５ g.３３a ７２ t.６７a ２４ 倐.６７abPierce ８４ !.６７a ８３ /.３３ab ６３ =.３３bc ４３ K.３３c ８４ Y.６７cd ８３ g.３３ab ６３ t.３３ab ４３ 倐.３３aCUF１０１ 谮８２ !.６７a ８４ /.００a ５４ =.６７b １８ K.００c ８２ Y.６７d ８４ g.００ab ５４ t.６７abc １８ 倐.００bcZhongmu No .１ 祆８０ !.６７a ６４ /.００ab ４２ =.００b １３ K.３３c ８０ Y.６７de ６４ g.００cd ４２ t.００bcd １３ 倐.３３bcdTable 2 Germination rate af ter trans f erred to distilled w ater in １０ alf alf a .Variety ０ mM ５０ mM １００ mM １５０ mMMaverick ０ 哌.００ ± ０ .００ ７ 葺.３３ ± ２ .３１ ２０  .６７ ± ３ .０６ ４０ .００ ± ４ .００Vernal １ 哌.３３ ± １ .１５ ２ 葺.６７ ± １ .１５ ２０  .６７ ± ４ .１６ ２８ .６７ ± １０ .２６Pioneer５４４６ 寣０ 哌.００ ± ０ .００ ６ 葺.６７ ± ３ .０６ １３  .３３ ± １ .１５ ３９ .３３ ± ３ .０６Legend ５ 哌.３３ ± ２ .３１ ４ 葺.６７ ± ２ .３１ １８  .００ ± ２ .００ ３６ .００ ± ４ .００Archer ４ 哌.６７ ± １ .１５ ６ 葺.６７ ± １ .１５ １０  .００ ± ２ .００ ３７ .３３ ± ８ .０８ABI７００ 圹０ 哌.６７ ± １ .１５ ２ 葺.００ ± ２ .００ １８  .００ ± ５ .２９ ４２ .６７ ± ９ .０２Dona Aan ０ 哌.００ ± ０ .００ ０ 葺.００ ± ０ .００ １７  .３３ ± ５ .３３ ３９ .３３ ± １０ .２６Pierce ０ 哌.００ ± ０ .００ １ 葺.３３ ± １ .１５ ８ 苘.６７ ± ３ .０６ ２３  .３３ ± ５ .７７CUF１０１ �２ 哌.６７ ± １ .１５ ３ 葺.３３ ± ２ .３１ ７ 苘.３３ ± ４ .１６ ３０  .００ ± ６ .００Zhongmu No .１  ３ 哌.３３ ± １ .１５ ７ 葺.３３ ± ３ .０６ ８ 苘.６７ ± １ .１５ ４６ .００ ± １２ .１７ReferenceJ .D .Rhoades , J .Loveday . ( １９９０ ) Salinity in irrigated agriculture .in American Society of Civil Engineers , I rrigation o fA gricultural Crops ( Steward B .A . and Nielsen ,D .R . ,eds) ,Am .Soc .Agronomists ,Monograph ３０ ,１０８９‐１１４２ .Supported by MOST :２００６BAD０１A１９ , ２００６BAD０４A０４　 　 　 　 　 MOE :２００６００２２０１１

Influence of Salinity on Seed Germination of 10 Alfalfa Varieties

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Influence of Salinity on Seed Germination of 10 Alfalfa Varieties

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