Auad, A. M.

Bellon, G.

Carvalho, Marcelo A.

Faleiro, Fabio G.

Junqueira, K. P.

Paula‐Moraes, S. V.

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Molecular Characterization of Spittlebug Mahanarva spectabilis (Distant, 1909: Hemiptera: Cercopidae) Populations Cccurring in "Legal Amazon" in Brazil S. V. Paula‐Moraes EMBRAPA, Brazil Fabio G. Faleiro EMBRAPA, Brazil G. Bellon EMBRAPA, Brazil K. P. Junqueira EMBRAPA, Brazil A. M. Auad EMBRAPA, Brazil See next page for additional authors Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/16-1/26 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. Presenter Information S. V. Paula‐Moraes, Fabio G. Faleiro, G. Bellon, K. P. Junqueira, A. M. Auad, and Marcelo A. Carvalho This event is available at UKnowledge: https://uknowledge.uky.edu/igc/21/16-1/26 瞯 ]824　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems——— Integrated Management of Harmful Organisms of Grasslands/RangelandsMolecular characterization of spittlebug Mahanarva spectabilis ( Distant , 1909 :Hemiptera :Cercopidae) populations occurring in �Legal Amazon" in BrazilS .V . Paula‐Moraes ; F . G . Faleiro ; G . Bellon ; K P . Junqueira ; A . M . A uad ; M . A . Carv alho .Braz ilian A gricultural Research Corporation , Embrapa Cerrados , BR 020 km 18 , PO Box 08223 , Planaltina‐DF , Braz il ,73310‐970 E‐mail : marcelo＠ cp ac .embrapa .brKey words : tropical forages ,brachiaria briz antha ,cerrados ,rapd markersIntroduction Recent reports have indicated the occurrence of the spittlebug Mahanarva in pastures of Brachiaria briz antha cv .′Marandú′ in Cerrados and transition areas , located in the�Legal Amazon" in Brazil . Paula‐Moraes et al . (２００６) identified thisspittlebug species as Mahanarv a spectabilis Distant ( １９０９ ) . Since then studies related to the aspects of the Mahanarv abioecology , economic impact , its geographic distribution , as well as information on the genetic inter and intra‐specificvariability have been done . Considering this , the correct identification of the nymphs and adults of the species occurring indifferent areas has extreme importance . Molecular markers have been used as a tool to support taxonomy studies and to accessthe genetic diversity of populations of insects and plants . So , the objective of this study was to validate a DNA exctrationprotocol of M . spectabilis stored in different conditions . It also had the objective to analyze the genetic similarity betweenrecollected nymphs and adults of M . spectabilis in different sites .Materials and methods Genomic DNA of adults and nymphs of M . spectabilis stored in alcohol ７０％ and １００％ was extractedusing a protocol proposed by Faleiro et al . ( ２００３) . These insects were recollected in ９ different sites located in Minas Gerais ,Distrito Federal ,Tocantins and Goiás states . After DNA quantification and a quality check two primers of ten nucleotideslength from the Operon Technologies kit were used to amplify genomic DNA . Amplification products were separated byelectrophoresis on １ .５％ agarose gels and banding patterns were visualized by staining the gels in ethidium bromide solutionsand viewing under UV radiation . For the analysis of genetic similarity RAPD markers of １８ specimens , a nymph and an adult ,collected in ９ places were analyzed . Genetic distance was later used as a criterion for differentiation among specimens to preparea cluster analysis .Results Amplifications were obtained using these two primers . Eighty‐five different RAPD bands were amplified . Theproportion of polymorphic RAPD loci was ９６ .５％ . The results of the genetic similarity indicate small differences amongnymphs and adults recollected in the same place . This result indicates that the nymph can be used to represent a population ofa specific collection point . This is very important , because when only nymphs are available , confident identification of thespecies is of ten compromised . In such occasions molecular markers become an important tool for inferences on the taxonomicidentification of the nymphs . No differences were observed among specimens stored in ７０％ and １００％ alcohol .Conclusions Molecular characterization was achieved with RAPD molecular markers , which proved to be very informative andefficient to characterize the genetic diversity and relationship among populations of the species . RAPD molecular markersproved to be a useful tool to support taxonomy studies and the corrected identification of insects of M . spectabilis . The resultsof this work offer the basis for future studies of the genetic variability , mapping of occurrence of the Mahanarv a spectabilis inpastures and population genetics studies .ReferencesFaleiro , F .G . ; Faleiro , A .S .G . ; Cordeiro , M .C .R . ; Karia , C .T . Metodologia para operacionalizar a extra毕本o de DNA deespécies nativas do cerrado . Planaltina : Embrapa Cerrados , ２００３ . (Comunicado Técnico ,N .９２) .Paula‐Moraes ,S .V . ;Carvalho ,G . S . ;Ramos ,A . K . B . ;Auad ,A . M . ;Takada ,S . ;Barcellos . A . O . Ocorrência dacigarrinha‐das‐pastagens Mahanarva spectabilis ( Distant , １９０９ ) em gramíneas forrageiras e sua istribui毕本o em áreas deCerrado e na Amaz蹦nia Legal . In : reuni本o anual da sociedade brasileira de zootecnia , ４３ , ２００６ , Jo本o Pessoa . Anais . . .Sociedade .Brasileira de Zootecnia . CD‐ROM . ２００６ . ５p ."

Molecular Characterization of Spittlebug \u3ci\u3eMahanarva spectabilis\u3c/i\u3e (Distant, 1909: Hemiptera: Cercopidae) Populations Cccurring in  Legal Amazon  in Brazil

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3694&amp;context=igc

Molecular Characterization of Spittlebug \u3ci\u3eMahanarva spectabilis\u3c/i\u3e (Distant, 1909: Hemiptera: Cercopidae) Populations Cccurring in Legal Amazon in Brazil

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