Cai, Yimin

Ennahar, Saïd

Noor, Ramli Mohd

Uegaki, Ryuichi

Xu, Chuncheng

Yoshida, Norio

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Fermentation Quality of Silage Prepared with Bacteriocin‐Producing Probiotic Lactic Acid Bacteria Yimin Cai National Institute of Livestock and Grassland Science, Japan Saïd Ennahar Université Louis Pasteur, France Ramli Mohd Noor Malaysian Agricultural Research and Development Institute, Malaysia Chuncheng Xu National Institute of Livestock and Grassland Science, Japan Ryuichi Uegaki National Institute of Livestock and Grassland Science, Japan See next page for additional authors Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/15-1/46 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. Presenter Information Yimin Cai, Saïd Ennahar, Ramli Mohd Noor, Chuncheng Xu, Ryuichi Uegaki, and Norio Yoshida This event is available at UKnowledge: https://uknowledge.uky.edu/igc/21/15-1/46 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　 瞯 693　 瞯Grasslands/Rangelands Production Systems ——— Forage Quality ,Conservation and UtilizationFermentation quality of silage prepared with bacteriocin‐producing probiotic lactic acid bacteriaYimin Cai1 , Sa甭d Ennahar 2 , Ramli Mohd Noor 3 Chuncheng Xu1 , Ryuichi Uegaki1 and Norio Yoshida11 National Institute o f Livestock and Grassland Science , Nasushiobara , Tochigi 329‐2793 , Japan , E‐mail :cai ＠ a f f rc .go .j p . 2 Université Louis Pasteur , 74 , route du Rhin , 67400 I llkirch , France . 3 Strategic Livestock Research Centre , MARDI ,P .O . Box 12301 , 50774 Kuala Lumpur , MalaysiaKey words : bactericin , lactic acid bacteria , silageIntroduction Bacteriocin‐producing lactic acid bacteria ( LAB) have a bactericidal effects against foodborne pathogens , and theyinhibit the outgrowth of bacterial spores of clostridia and bacilli . Most bacteriocins produced by LAB are degradable in humantract and thermoresistant . In the present experiment , the application of bacteriocin‐producing LAB with strong antimicrobialactivity against undesirable microorganisms as probiotics for silage preparation were studied .Materials and methods Rice straw , forage paddy rice and Italian ryegrass ( Lolium multi f lorum ) were chopped into １０‐mmlengths and three replicates of the same forage were used for microbiological analysis . Silages were prepared using a small‐scalesystem of silage fermentation ( Ennahar et al . ２００３ , Cai et al . １９９９ ) . Lactococcus lactis RO５０ was added at a rate of １ .０ ×１０５ colony forming unit ( cfu) g ‐１ of lactic acid bacteria ( LAB) on a fresh matter basis .Results Strain RO５０ was found to display anti‐microbial activity against a variety of bacteria , including clostridia , bacilli andLAB , but showed respectively wide and narrow spectra of activity .Table 1 Fermentation quality and microorganism composition o f silage 倡 .Rice straw Forage paddy rice Italian ryegrassControl R０５０ Control R０５０ Control R０５０Dry matter ( ％ ) ３１ .９ ３２ .１０ ２５ .９０ ２６ .１０ １９ .５６ １８ .９２pH ５ .４０a ４ .３５b ５ .４０a ４ .１４b ４ .６０a ３ .９５bLactate ,％ FM ０ .３２b ０ .８７a ０ .４６b １ .１４a ０ .５５b １ .４１aAcetate ,％ FM ０ .５３ ０ .３５ ０ .５３ ０ .５５ ０ .３３ ０ .３５Butyrate ,％ FM ０ .６７ nd ０ .３５ nd ０ .３７ ndAnmmonia‐N ,g / kg FM ０ .６２a ０ .３８b ０ .５７a ０ .４０b ０ .５５a ０ .２８bLactic acid bacteria ,log cfu/ g FM ６ .７０b ６ .８０a ５ .４０b ６ .８０a ５ .８３b ６ .４０aR０ ５０ , log cfu/ g FM nd ６ .５０ nd ４ .３０ nd ５ .８０Mould ,log cfu/ g FM ４ .２０ nd nd nd nd ndClostridia ,lod cfu/ g FM ３ .２０ nd ３ .６０ nd ４ .３０ nd倡 Silage stored for ６０ day , FM : fresh matter , nd : not dectected .a ,b , : means with cdum of same silage with different letter difter ( P ＜ ０ .０５ ) .Rice straw , forage paddy rice and Italian ryegrass silages inoculated with strain RO５０ were well preserved and exhibited lowerpH , butyric acid and ammonia‐nitrogen , and higher lactic acid content , as compared to the control silage . During the silagefermentation process , the inoculated silages exhibited increased numbers of LAB and reduced clostridia compared to that ofcontrol ( Table １ ) .Conclusions The use of such bacteriocinogenic LAB inoculants to improve the silage fermentation is highly recommended . Theaddition of strain RO５０ at ensiling is intended to ensure rapid and vigorous fermentation resulting in a faster accumulation oflactic acid , lower pH values at earlier stages of ensiling , and inhibition of growth of some pathogenic bacteria . Therefore , thebacteriocin‐producing Lactococcus lactis RO５０ are very beneficial as potential silage inoculants .ReferencesEnnahar S , Cai Y , Fujita Y ( ２００３ ) Phylogenetic Diversity of Lactic Acid Bacteria Associated with Paddy Rice Silage asDetermined by １６S Ribosomal DNA Analysis . A p pl . Environ . Microbiol . ６９ (１ ) : ４４４‐４５１ .Cai , Y , Kumai S , Ogawa M , Benno Y , Nakase T (１９９９ ) Characterization and identification of Pediococcus species isolatedfrom forage crops and their application for silage preparation . A p p l . Environ . Microbiol . ６５ : ２９０１‐２９０６ .

Fermentation Quality of Silage Prepared with Bacteriocin‐Producing Probiotic Lactic Acid Bacteria

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Fermentation Quality of Silage Prepared with Bacteriocin‐Producing Probiotic Lactic Acid Bacteria

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