Dai, Y.

Deng, Bo

Li, Guizhen

Yang, F. Y.

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Effect of Lactic Acid Bacterial Inoculant on Nitrogen Change and in Vitro Digestibility of Alfalfa Silage Y. Dai Jinlin Agricultural University, China Guizhen Li Gansu Agricultural University, China F. Y. Yang China Agricultural University, China Bo Deng China Agricultural University, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/15-2/1 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 698　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems ——— Forage Quality ,Conservation and UtilizationEffect of lactic acid bacterial inoculant on nitrogen change and in vitro digestibility of alfalfasilageY . Dai1 , Guizhen Li2 ， F .Y .Yang 3 , B .Deng 3 倡1 Jinlin Agricultural University , Changchun , China ,2 Gansu Agricultural University , Lanzhou , China ,3 Institute o fGrassland Science , China Agricultural University , Bei j ing , China . E‐mail : dengbo67 ＠ cau .edu .cnKey words : alfalfa silage , nitrogen change , in vitro , digestibilityIntroduction Alfalfa ( Medicago sativa ) is a quality forage both for hay and silage . However , Protein hydrolysis occurs duringfermentation process , caused ４４ ％ to ８７ ％ protein to degrade into indigestible nitrogen‐NPN ( Nonprotein Nitrogen ) , as theresult , it is associated with nutrient losses( Papadopoulos et al . , １９８３ ) .The objective of this study was to determine the effectsof MicroManager , a silage additive ,on preservation and digestibility of alfalfa silage making .Materials and methods Alfalfa harvested at flowering stage with dry matter content of ３０ ％ was chopped to １ .５ cm length .Microbial inoculant silage additive ( MicroManager ,Medipharm USA , Des Moines , Iowa ) was applied during filling at fourrates ０ g / t ( Control) , １１ .３５g / t ( Level I) , ２２ .７g / t ( Level II) and ３４ .０５g/ t ( Level III) . The MicroManager contained specificstrains , with Lactobacillus plantarum ( minimum total cfu of LAB at ５ × １０９ / g ) . Then fresh materials were incubated in a ３０ ℃water bath for ４０ days . The pH ,DM ,CP ,WSC , NPN , NH３‐N , FAA‐N , Peptide‐N the rate of in vitro DM 、CP 、NAF andADF digestion were tested after incubation .Results Compared with the control , pH and NPN (６０ ％ of Total N ) were significantly low , especially in level II and level IIItreatment , which suggested protein hydrolysis was inhibited with the application of microbial inoculants ( Table １ ) . Themicrobial inoculant silage additive remarkably enhanced digestibility , especially in Level III treatment ( Table ２ ) . The in vitroDM , CP , NDF and ADF digestibility were １３ .２５ ％ , ５ .７４ ％ ,７４ .４１ ％ and ６７ .７４ ％ higher , respectively , in level III than thosein the control .Table 1 Comparison o f nitrogen changes o f al f al f a herbage and silage .Index Before Silage After silageControl Ⅰ Ⅱ ⅢTotal N ( g Kg‐１ DM) ２９ .０２ ２７ .５１a ３０ .５６b ２９ .２５b ３０ .９６bNPN( g Kg‐１ TN) ２９１ .２２ ６３９ .０２a ６１９ .０６a ６０１ .９９b ５６８ .８１cNH３‐N( g Kg‐１ TN) ７ .９８ ２０９ .１７a ８１ .８２b ８６ .９８b ８５ .４７bAA‐N( g Kg‐１ TN) ３４ .３７ ３９９ .００a ３２５ .１３b ２７０ .４６c ２７４ .４８bcPeptide‐N( g Kg‐１ TN) ２４８ .８７ ３０ .８５b ２１２ .１２a ２４４ .５５a ２０８ .８６apH ６ .０７ ５ .４８a ４ .４２b ４ .３８b ４ .４０bTable 2 Degradation rate o f DM 、CP 、NAF 、ADF o f al f al f a silage in vitro .Degradation rate( ％ ) Control Ⅰ Ⅱ ⅢDM ５６ .１４b ６１ .７９a ６１ .２７a ６３ .５８aCP ８２ .２２b ８７ .６０a ８７ .１５a ８６ .９４aNDF ２３ .２１c ３２ .５８b ３１ .４５b ４０ .４１aADF ２４ .９３c ３３ .６６b ３２ .２７b ４１ .８２aConclusion Applying bacterial inoculants ( such as lactic acid bacterial inoculant ) in alfalfa silages may decreas proteindegradation and improved silages quality and nutritive value , and increased digestibility . This observation agreed with Pys摧sfindings that the processes of protein degradation was significantly reduced in alfalfa‐rolled barley silages made with the additionof lactic acid bacteria and enzyme inoculants (２００２ ) .ReferencesPapadopoulos Y A , McKersie B D . A comparison of protein degradation during wilting and ensiling of six forage species [ J ] .Can . J .Plant Sci . ,１９８３ ,６３ : ９０３‐９１２ .Pys J ,Migdal W . Effect of lactic acid bacterial inoculant with enzyme and rolled barley additive on the chemical compositionand protein degradation of alfalfa silage . Biotechnology in Animal Husbandry ,２００２ ,１８ :３‐４ ,３３‐４４ ,３１ .

Effect of Lactic Acid Bacterial Inoculant on Nitrogen Change and \u3ci\u3ein Vitro\u3c/i\u3e Digestibility of Alfalfa Silage

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3595&amp;context=igc

Effect of Lactic Acid Bacterial Inoculant on Nitrogen Change and \u3ci\u3ein Vitro\u3c/i\u3e Digestibility of Alfalfa Silage

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