Chung, Eui-soo

Ham, Jun-sang

Kim, Jong-geun

Kim, Meing-joong

Seo, Sung

Yoon, Sei-hyung

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Comparison on the Newly Developed Silage Inoculant (Lactobacillus plantarum NLRI 401) for Whole Crop Rice Silage Jong-geun Kim National Institute of Animal Science, South Korea Eui-soo Chung National Institute of Animal Science, South Korea Jun-sang Ham National Institute of Animal Science, South Korea Sei-hyung Yoon National Institute of Animal Science, South Korea Meing-joong Kim National Institute of Animal Science, South Korea See next page for additional authors Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/15-2/16 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. Presenter Information Jong-geun Kim, Eui-soo Chung, Jun-sang Ham, Sei-hyung Yoon, Meing-joong Kim, and Sung Seo This event is available at UKnowledge: https://uknowledge.uky.edu/igc/21/15-2/16 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　 瞯 713　 瞯Grasslands/Rangelands Production Systems ——— Forage Quality ,Conservation and UtilizationComparison on the newly developed silage inoculant ( Lactobacillus plantarum NLRI 401) forwhole crop rice silageJong Geun Kim , Eui Soo Chung , Jun Sang Ham , Sei H yung Yoon , Meing Joong Kim , Sung SeoRural Development Administration , National Institute o f Animal Science , Cheonan 330‐801 , Korea ,E‐mail : jonggk ＠ rda .go .krKey words : whole crop rice , silage , inoculant , organic acid , qualityIntroduction In ２００７ , approximately five million tons of rice was produced in Korea , but the consumption of rice has decreased .The government has been considering the matter from many angles and farmers havealso been considering the problem . Arelatively new method of wrapping a large round bale with stretch‐wrap plastic films to make silage is an increasingly popularmeans of preserving forage . However , it is difficult to make good silage from whole crop rice due to low water solublecarbohydrate (WSC) content at harvest ( Cai , ２００６ ) . Lactic acid bacteria play a key role in making silage from forage , andlactic acid bacteria selected from good silage could be expected to be suitable inoculant for making good silage .Materials and methods Lactic acid bacteria were isolated from good barley silage by plating on MRS agar containing ０ .０２ ％sodium azide , and were screened for growth and acid producing ability . The viable cell counts were measured by the plate countmethod . Gram stain and morphology were examined after ２４ hours of incubation on MRS agar . Catalase activity , growth atdifferent pH values , gas production from glucose , and sugar fermentation were determined by the methods described in Speck(１９８４ ) . Whole crop rice was ensiled at ripening stage . The crop was ensiled in an experimental silo (２０ litters) with or withoutmicrobial additives ( NLRI ４０１ , HM/ F and P１１３２ ) and stored at room temperature for ６０days . A １０g sample of silage wasmacerated with １００ml of distilled water , filtered through a filter paper ( ＃ No .６ ) . The pH was measured with a pH meter ( HI９０２４ ; Hanna Instrument Inc . UK ) . Volatile fatty acids were analyzed by using gas chromatography ( Model ３４００ ; VarianCo . , USA) . Lactic acid was analyzed by using HPLC ( HP‐１１００ ; Hewlett‐Packard Co . , USA) .Results The quality of silages was summarized in Table １ . After ２ months , NLRI ４０１ and p １１３２ inoculated whole crop silageshowed lower pH and butyric acid , and higher lactate content than control and HM/ F inoculant treated silage . The qualitygrade of NLRI ４０１ and P １１３２ treated silage was excellent , but control was poor . It also showed that all inoculated silages werein low butyric acid content .Table 1 Acidity( p H ) , organic acid content and Flieg摧s score o f whole crop rice silages in relation to di f f erent inoculanttreatment .Item pH Organic acid( ％ in DM)Acetic Butyric LacticFlieg摧sscoreQualitygradeControlP１１３２HM/ FNLRI ４０１５ .７３４ .６１４ .７２4 .67０ .４６０ .２８０ .３１0 .23０ .１６０ .０６０ .０６0 .05２ .４６２ .８７２ .７２2 .92６１８３８０88２１２1倡 Quality grade : Flieg摧s score １００‐８１ (１ ) , ８０‐６１ (２ ) , ６０‐４１ (３ ) , ４０‐２１ (４ ) , below ２０ (５ )

Comparison on the Newly Developed Silage Inoculant (\u3ci\u3eLactobacillus plantarum\u3c/i\u3e NLRI 401) for Whole Crop Rice Silage

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Comparison on the Newly Developed Silage Inoculant (\u3ci\u3eLactobacillus plantarum\u3c/i\u3e NLRI 401) for Whole Crop Rice Silage

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