Hao, Tingjie

Li, Linhang

Liang, Yunzhang

Ma, Yubao

Wu, Zuogong

Xie, Jihong

Xu, Zhu

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Effect of Ion‐Beam Implantation on Seed Vigor of Roegneria Kamoji Ohwi Jihong Xie Chinese Academy of Agricultural Sciences, China Zuogong Wu Inner Mongolia Key Laboratory of Ion Beam Bioengineering, China Yunzhang Liang Inner Mongolia Key Laboratory of Ion Beam Bioengineering, China Zhu Xu Chinese Academy of Agricultural Sciences, China Linhang Li Chinese Academy of Agricultural Sciences, China See next page for additional authors Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/14-2/13 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. Presenter Information Jihong Xie, Zuogong Wu, Yunzhang Liang, Zhu Xu, Linhang Li, Tingjie Hao, and Yubao Ma This event is available at UKnowledge: https://uknowledge.uky.edu/igc/21/14-2/13 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　 瞯 ]611　 瞯Grasslands/Rangelands Production Systems——— Seed Science and TechnologyEffect of ion‐beam implantation on seed vigor of Roegneria Kamoj i OhwiX ie Ji‐hong1 ,W u Zuo‐gong2 ,L iang Yun‐z hang2 ,X u Zhu1 ， L i‐L inhang1 , H ao T ing‐j ie 2 and Ma‐Yubao11 G rassland Research Institute o f Chinese A griculture Science , H uhhot , 010018 , P .R . China , E‐mail : x ie j ihong223＠ 163 .com , 2 Inner Mongolia Key L aboratory o f Ion Beam Bioengineering , H uhhot , 010018 , P .R . China ,E‐mail : wuz uogong＠163 .comKey Words :Roegneria Kamoji Ohwi ,ion‐beam ,seed vigor ,damage effect ,saddle curveIntroduction Roegneria K amoj i Ohwi is a cross‐pollinated , long lived and perennial species in Roegneria , T riticeae tribe ofGramineae , has multi‐florets and multi‐seeds and has resistance against humidity and fusarium head blight . Domestic researchwas focused mainly on genetic variation in morphology , cytology , isoenzyme , RAPD and SSR( Zhou ,２０００ ) . Ion‐beam , as anew source of mutation , has been characterized by its higher mutation rate and wider mutational spectrum with lower damagefor the implanted organism ( Yuan et al . ２００３ ) . In this study , ion beam is implanted in seeds to provide abundant mutatedcolonies for the genetic improvement of Roegneria K amoj i Ohwi .Materials and methods Seeds of Roegneria K amoj i Ohwi T０５０３ were provided by professor Xu Zhu of the Grassland ResearchInstitute of Chinese Agricultural Science . The implantation was performed with the Ion Beam Bioengineering Instrument atInner Mongolia University . The seeds were placed into the target chamber of the implantation machine . When the pressure ofvacuum of the target chamber reached around １ .６ ～ ２ .６ × １０‐２ Pa , the seeds were implanted with low energy (２５ keV) nitrogenion beams under the flux of ２ .６ × １０１３ ions/ cm２ per pulse . The fluence ranged from ５００ × ２ .６ × １０１３ ions/ cm２ to ５０００ × ２ .６ ×１０１３ ions/ cm２ .Results Both the germination energy and germination rate showed Saddle Curve as the doses increased ( Figure １ ) . The seedgermination of Roegneria K amo j i Ohwi increased initially at the lower doses of ５００ × ２ .６ × １０１３ ions/ cm２ and １ ,０００ × ２ .６ × １０１３ions/ cm２ ,followed by a declining trend at higher doses . The sub‐lethal dose for germination rate was ３ ,５００ ( ２ .６ ( １０１３ ions/cm２ . The highest dose of ５０００ × ２ .６ × １０１３ ions/ cm２ cause severe damage to the seeds resulting in a germination rate of１１畅８％ . The seeds treated with low doses germinated faster and grew better , while the seeds treated with high doses hadslower germination and weaker grow th . The vigor index can be used to represent physiological damages at seedling stage afterion beam implantation . In this study , most doses improved the seed vigor index . Implantation with the dose of １０００ × ２ .６ ×１０１３ ions/ cm２ resulted in the highest seed vigor index ( Figure ２ ) .Figure 1 Germination energy and rate as a f unction o f dose .　 　 　Figure 2 V igor index as a f unction o f dose .Conclusions The effects of icon beam implantation on the seeds of the Roegneria K amoj i hwi depends on doses . Doses differedsignificantly in their effects on the germination energy , germination rate and vigor index . Ion‐beam implantation with a lowdose can bring excitation effect and resulted in high germination rate and energy . With suitable energy and dose , the ion‐beamimplantation can obviously improve performance and quality of the contemporary crop . However , if ion‐beam implantation isused to induce genetic mutation or assist genetic improvement , higher doses shall be used( Yang et al . ,２００６) . In this research ,the vigor index is still high even at the medium and high doses (１ ,５００ to ４ ,０００ × ２ .６ × １０１３ ions/ cm２ ) , and the author thinkshigher doses have mutagenesis effects on Roegneria K amo j i Ohwi .ReferencesZhou Yonghong , ２０００ . A biosystematic study on Roegneria dolichathera , R . pendulina and R . kamoji . ACTAPRATACULTURAL SCIENCE ,２ ,４５‐５２ .Yuan Chengling , Yu Zengliang , ２００３ . The Grand Progress of Low‐Energy Ion Beam in Life Sciences . Progress InBiotechnology ,４ ,５７‐６１ .Yang Gen ,Bian Po ,Zhang Zhenghong ,Ren Jie ,Wu Lijun ,Yu Zengliang ,２００６ . Bioeffects of Low Energy Ion‐Beam Implantationand Vacuum Treatment on M１ Arabidopsis Thaliana . Plasma Science & Technology ,８ ,２４２‐２４５ .

Effect of Ion‐Beam Implantation on Seed Vigor of \u3ci\u3eRoegneria Kamoji\u3c/i\u3e Ohwi

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3581&amp;context=igc

Effect of Ion‐Beam Implantation on Seed Vigor of \u3ci\u3eRoegneria Kamoji\u3c/i\u3e Ohwi

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