da Silva Ávila, Carla Luiza

Figueiredo, H. C. P.

Morais, A. R.

Pereira, Odilon G.

Pinto, José C.

Schwan, R. F.

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Microbiological Profile of Sugarcane Silages Inoculated with Lactobacillus buchneri José C. Pinto Universidade Federal de Lavras, Brazil Carla Luiza da Silva Ávila Universidade Federal de Lavras, Brazil H. C. P. Figueiredo Universidade Federal de Lavras, Brazil A. R. Morais Universidade Federal de Lavras, Brazil Odilon G. Pereira Universidade Federal de Viçosa, Brazil See next page for additional authors Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/15-1/3 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. Presenter Information José C. Pinto, Carla Luiza da Silva Ávila, H. C. P. Figueiredo, A. R. Morais, Odilon G. Pereira, and R. F. Schwan This event is available at UKnowledge: https://uknowledge.uky.edu/igc/21/15-1/3 瞯 650　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems ——— Forage Quality ,Conservation and UtilizationMicrobiological profile of sugarcane silages inoculated with Lactobacillus buchneriJ . C . Pinto1 , C . L . S . 搀 vila1 , H . C . P . Figueiredo1 , A . R . Morais1 , O . G . Pereira2 , R . F . Schwan11 Universidade Federal de Lavras and 2 Universidade Federal de Vi毕osa , Minas Gerais , Braz il .E‐mail : josecard ＠ u f la .brKey words : fermentation ,filamentous fungi ,lactic acid bacteria ,Saccharum spp . ,yeastsIntroduction Sugarcane ( Saccharum spp .) ensiling is recommended with the objective of making its management more ease onthe farm . Nevertheless , over the processing of its ensiling occurs intense alcoholic fermentation by yeasts , resulting into highlosses of dry matter ( DM ) . Inoculants containing the species L . buchneri have proved effective in inhibiting the growth ofyeasts in silages as related to the increased production of acetic acid or lactic acid ( Filya , ２００３ ) . The compatibility between theforage plant and microorganisms is a factor determining for the successful application of microbial inoculants in silages . Theobjective of this work was to evaluate the effect of two strains of L . buchneri , one from a commercial inoculant and the otherisolated from the local sugarcane silage , on the fermentation profile of this forage plant .Materials and methods The experiment was conducted in the Animal Science and Biology Departments of the Federal Universityof Lavras ‐ MG , Brazil , following to a completely randomized design with three replicates in factorial scheme of the ３ x ６ type ,that is , three silages and five times of silo opening . The treatments were : sugarcane silages without any inoculant , with anexperimental inoculant and with a commercial inoculant . The inoculants were previously prepared in the MicrobiologyLaboratory and added at the population of １０８ cfu/ g . For the evaluation of the fermentation profile of the silages , the silos wereopened with ０ , ３ , １０ , ３０ , ６０ and ９０ days of fermentation and , at each time , samples were taken for evaluation of thepopulation of lactic acid bacteria ( LAB) , yeasts and filamentous fungi , by surface plating .Results In Figure １ , it is found that at time ０ , the forage without an inoculant showed a lower population of LAB (３ .６ log cfu/g of forage) than the inoculated forages . The yeast and filamentous fungi populations were similar to the three silages withrespective average values of ７ .７８ and ５ .４ log cfu/ g of forage . At ３ days fermentation occured an increase in the population ofLAB , comparing to those of the inoculated silages . This increase in LAB is common and was observed in previous studies .From the third to the tenth days of fermentation , a new fall in the population of LAB , its being more marked in the silagewithout an inoculant . This presented a high population of yeast , along the fermentative process , there being no inhibition byanaerobiosis or acidity . The addition of inoculants caused a similar effect upon the growth of yeasts , with a linear decrease tillthe ９０ days fermentation , when the population were bellow the detectable level ( ＜ ２ .０ log cfu/ g ) . Throughout thefermentation process , the population of filamentous fungi were bellow the detectable level ( ＜ ２ .０ log cfu/ g ) .　 　 　 　 　 　 　Figure 1 Populations o f L AB (a) and yeasts (b) o f the sugarcane silages without an inoculant (１ ) and with ex perimental (２ )and commercial (３ ) inoculants along the f ermentation p rocess .Conclusion The two inoculants improved in a similar manner , the microbiologic profile of sugarcane silages .Acknowledgement Research supported by FAPEMIGReferenceFilya , I . The effect of Lactobacillus buchneri with or without homofermentative lactic acid bacteria , on the fermentation ,aerobic stability and ruminal degradability of wheat , sorghum and maize silages . Journal o f A p plied Microbiology . v . ９５ ,p .１０８０‐１０８６ , ２００３ .

Microbiological Profile of Sugarcane Silages Inoculated with \u3ci\u3eLactobacillus buchneri\u3c/i\u3e

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3509&amp;context=igc

Microbiological Profile of Sugarcane Silages Inoculated with \u3ci\u3eLactobacillus buchneri\u3c/i\u3e

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