Arcioni, Sergio

Damiani, F.

Paolocci, F.

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Exogenous Transcription Factors Alter the Patterns of Condensed Tannin Accumulation in Lotus corniculatus Sergio Arcioni Consiglio Nazionale delle Ricerche‐Istituto Genetica Vegetal, Italy F. Damiani Consiglio Nazionale delle Ricerche‐Istituto Genetica Vegetal, Italy F. Paolocci Consiglio Nazionale delle Ricerche‐Istituto Genetica Vegetal, Italy Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/15-1/5 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 652　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems ——— Forage Quality ,Conservation and UtilizationExogenous transcription factors alter the patterns of condensed tannin accumulation in LotuscorniculatusA rcioni S . , Damiani F . , Paolocci F .Consiglio Nazionale delle Ricerche‐Istituto Genetica Vegetale , via Madonna Alta 130 , 06128 Perugia , I taly , E‐mail :sergio .arcioni ＠ igv .cnr .itKey word :genetic transformation ,bHLH transcription factors ,MYB transcription factorsIntroduction Condensed tannins ( CT ) are secondary metabolites useful in forage legumes to prevent protein degradation andruminal bloating . The synthesis and accumulation of such compound is limited to seed coat in alfalfa and clover . Conversely ,accumulation of CT in leaves occurs in some Lotus species and few other minor legumes . Understanding the genetic mechanismof CT synthesis and accumulation is the prerequisite to transfer the trait into the most important forage legumes . To this aimL . corniculatus plants polymorphic for CT accumulation in leaves were transformed with an anthocyanin activator bH L Htranscription factor from maize ( Sn) and a flavonoid repressor MYB transcription factor from Fragaria vesca ( FaMYB1 ) .Here we report phenotypical effect of transformation and alteration in the expression of late genes of the pathway (DFR , LAR ,ANR) previously characterised in our lab ( Paolocci et al . , １９９９ ; ２００７ ) .Materials and methods S５０ and S４１ plants , showing low and high leaf CT levels , respectively , were isolated from cv Leo andused as recipient genotypes for Agrobacterium rhi zogenes‐mediated transformation .Each transcription factor was driven by theconstitutive promoter CAMV３５S . Transformation and regeneration protocols and leaf‐CT staining were performed according toPaolocci et al . (１９９９ ) , gene expression analysis by real time RT‐PCR as described by Paolocci et al . (２００７ ) .Results and discussion Transformation modified CT content in leaves as reported in Table １ . Transformation deeply modify CTlevels . S４１ depletes CT level after transformation with the negative regulator . S５０ transformed with Sn increases CT . Theeffects of transformation is more evident in contrasting genotypes , i .e . Sn does not increase CT in S４１ and FaMYB1 poorlydepletes CT levels in S５０ ( not shown) .Table 1 CT levels in trans f ormed and control p lants .S５０ S５０ Sn S４１ S４１FaMYB１％ CT cells ０ .５０ ０ .９１ ０ .９９ ０ .２２In Figure １ it is reported the expression levels of key genes of the CT pathway . It is worth of mention that Sn enhances ,whereas , FaMYB1 reduces , the expression of the structural genes .Figure 1 Gene ex p ression .Conclusion Results suggest the possibility of inducing CT accumulation in alfalfa by altering the expression levels of endogenousactivator or repressor genes .ReferencesPaolocci F , Capucci R , Arcioni S , Damiani F ( １９９９ ) Birdsfoot trefoil : a model for studying the synthesis of condensedtannins . In : Plant Poly phenols 2 . K luwer Academic New York pp . ３４３‐３５６ .Paolocci F , Robbins M .P . , Madeo L . , Arcioni S . , Martens S . , Damiani F . (２００７ ) Ectopic Expression of a Basic Helix‐Loop‐Helix Gene ......in Lotus corniculatus . Plant Physiol . １４３ : ５０４‐５１６ .

Exogenous Transcription Factors Alter the Patterns of Condensed Tannin Accumulation in \u3ci\u3eLotus corniculatus\u3c/i\u3e

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3511&amp;context=igc

Exogenous Transcription Factors Alter the Patterns of Condensed Tannin Accumulation in \u3ci\u3eLotus corniculatus\u3c/i\u3e

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