Huang, Junsheng

Yi, Kexian

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Genetic Diversity Analysis of Colletotrichum gloeosporioides from Stylo and Anthracnose Resistant Breeding Kexian Yi Chinese Academy of Tropical Agricultural Sciences, China Junsheng Huang Chinese Academy of Tropical Agricultural Sciences, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/13-2/6 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　 瞯 ]509　 瞯Grasslands/Rangelands Production Systems——— Domestication of Native Grasslands/Rangelands Plants for Regional UseGenetic diversity analysis of Colletotrichum gloeosporioides from Stylo and Anthracnoseresistant breedingY I Kex ian , HUA NG JunshengChinese A cademy o f T rop ical A gricultural Sciences , H ainan 571737 , China , E‐mail :y ikex ian＠ 21cn .comKeywords : Stylosanthes ,Colletotorichum gloeosporioides , RAPD ,AFLP ,virulence ,epidemiology ,resistant breedingIntroduction Stylosanthes spp is the most important and commercial forage legume in tropical area in China . It is widely used asforage , green manure , cover crops , erosion control , hay and meal commercial production . However , stylo anthracnose , mainlycaused by a fungus Colletotrichum gloeosporioides , is the most severe disease in stylo production which has resulted in seriousdamage in China in the past ２０ years . More than ３ productive varieties have been given up because of anthracnose .Materials and methods A field survey on stylo anthracnose was carried out widely in all the stylo production sites in China in thelast ten years and samples of infested plant materials were collected for isolation by pure‐culture and single sporing . ３３８ isolatesof Colletotrichum gloeosporioides were selected randomly for RAPD and AFLP analysis from the isolates bank in CATAS and１０４ isolates were selected for race typing based on virulence and pathogenic variation identification by using a set of hostdifferientials . Disease development with time was also observed regularly for epidemiological research . Base on the resultsabove , anthracnose resistant breeding for new varieties by different methods was implemented .Results and discussions１ . Total ７０３ isolates of Colletotrichum gloeosporioides have been collected . １１４ isolates were selected for RAPD analysis and１８１ isolates for AFLP . In addition , ４３ for RAPD which were clustered and compared with ２７６ isolates abroad . The isolatescollected in China were grouped in clusters Ⅱ , Ⅲ and Ⅵ based on six clusters of isolates from South America and othercountries . However , both of １１４ isolates for RAPD and １８１ siolate for AFLP were grouped in four clusters .‐Two mollecularmarkers showed a significant DNA polymorphism and genetic diversity among the tested isolates from China .２ . The results showed different virulence in different isolates . Total ４７ of type A isolates were grouped in ３ races and ５７ oftype B in ２ races . The results from race typing also showed that a wide pathegenic variation existed in type B . Thus moreattention should be paid to the monitoring and evaluation on pathogens .３ . An on‐station observation on epidemyology for three years showed the severity and incidence of stylo anthracnose weresignificantly correlated with air humidity ( r ＝ ０ .４４０３７ ,Pr ＝ ０ .０４５７ ) . There were two peaks of disease incidence in a year . Themain peak was in August and September , the other in Jannuary which followed the rainfall .４ . ３６ new accessions were evaluated and compared based on the anthracnose resistance from ２００１ to ２００３ . ５ lines from S .guianensis and ３ accessions from S .Seabrana and Seca were selected with higher anthracnose resistance and good yieldperformance .５ . Seeds from seven stylo species were treated by ６０Cο‐γ ray at ８ dose levels for suitable radioactive dose determination and newlines selection with high anthracnose resistance . １０ .３２ ～ １５ .４８C/㎏ was the suitable dose for stylo . １２８ lines with highestdisease resistance were selected by inoculation .Conclusion It is concluded that the genetic diversity , race types and field epidemiology of Colletotrichum gloeosporioides fromstylo in China have been clear initially and tens of new lines with high anthracnose resistance selected from this research havepotential for new cultivars in the future .ReferencesP .L .Weeds , S .Chakraborty , C .D .Fernandes , M .J .Charchar , C .R .Ramesh ,Y .Kexian ,and S .Kelemu , ２００３ ,GeneticDiversity .in Colletotrichum gloeosporioides from Stylosanthes spp . at Centers of Origin and Utilization . phytopathology . ９３ ( ２ ) １７６ ～１８５ .Xue‐qun Shi , Hai‐chao Song , Xin Zhang , Kexian Yi , ２００７ , Genetic Diversity Analysis of Hainan and Guangxi Styloanthracnose Pathogens Using Random amplified Polymorphic DNA .Chinese Bulletin o f Botany .２４ (２) :１７３ ～ １８０ .

Genetic Diversity Analysis of \u3ci\u3eColletotrichum gloeosporioides\u3c/i\u3e from Stylo and Anthracnose Resistant Breeding

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Genetic Diversity Analysis of \u3ci\u3eColletotrichum gloeosporioides\u3c/i\u3e from Stylo and Anthracnose Resistant Breeding

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