XiLin, T. Y.

Xu, Zhu

Yan, W. H.

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Study on Genetic Diversity of Wild Lespedeza Germplasm Resources W. H. Yan Chinese Academy of Agricultural Sciences, China Zhu Xu Chinese Academy of Agricultural Sciences, China T. Y. XiLin Chinese Academy of Agricultural Sciences, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/13-2/9 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 ]506　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems——— Domestication of Native Grasslands/Rangelands Plants for Regional UseStudy on genetic diversity of wild Lespedeza germplasm resourcesW . H .Y an1 , Z . X u1 , T .Y . X iL in1 ,21 G rassland Research Institute , CA A S , H uhhot , Inner Mongolia 010010 , China ,2 Pratacultural College , Gansu A griculturalUniversity , L anz hou 730070 , China , E‐mail : yxuz hu＠ yahoo .comKey words : Lespedez a Michx . , genetic diversity , RAPD marker , protein marker , morphological markerIntroduction Lespedez a Michx . are perennial plants which belongs to Leguminosae , it has excellent characteristics ( Clewell ,１９６４ ; Hoveland and Donnelly ,１９８５ ) , but little is known about its genetic diversity . The aim of the study was to identifygenetic differences between ２０ wild germplasm materials of Lespedez aMichx . including ３ species and １ variety , collected fromInner Mongolia , Shanxi , Hebei ,Gansu and Ningxia provinces in China using three approaches : morphology marker ; seedstorage protein marker and DNA marker .Materials and methods Using the same criterion , morphological data were collected for １９ quantitative characters and then thegenetic diversity based on seed storage protein marker and DNA marker among ２０ wild germplasm materials were tested bySDS‐PAGE and RAPD . Statistical analysis was made by SPSS１１ .５ , NTSYS‐pc and Popgene Version ３２ .Results and discussion The genetic diversity of Lespedez a Michx . is very abundant , the diversity of intra‐population is higherthan that of inter‐population . We can conclude that variation between populations is the main reason that results in variation ofLespedez a Michx . Among ３ species and １ variety , the genetic diversity of L . potaninii Vass . was the highest ( Zhang ,２００３ ;Zhao ,２００６) . The relationship among ３ species and １ variety was estimated by the statistical analysis of the diversity parametersand cluster analysis . The results from three approaches showed that the relation between L . hedysaroides ( Pall .) Kitag . andL . hedysaroides ( Pall .) Kitag . var . subsericea ( Kom .) Kitag . was closer ; for L . davurica ( Maxim .) Schindl . and L .potaninii Vass . was also closer , but L . hedysaroides ( Pall .) Kitag . var . subsericea ( Kom .) Kitag . was even closer than L .hedysaroides ( Pall .) Kitag . Cluster analysis was made with the un‐weighted pair group method for arithmetic averages( UPGMA) using Euclid distance coefficients which based on １９ morphological characters , using the symmetric matrix ofsimilarity coefficients based on seed storage protein patterns and RAPD marker . The three dendrograms indicated that therelationships among ２０ wild germplasm materials were basically confirmed on the traditional taxonomy of the species and inter‐species which material was collected .Conclusions We conclude the following by using the three evaluation approaches : ( １) there is a large amount of genetic diversityin Lespedez a Michx . germplasm resources , either within or between populations , although the variation between populationswhich was significantly higher than that within populations was the main cause that resulted in variation of Lespedez a Michx .germplasm resources . (２ ) The genetic diversity of L . potaninii Vass . was highest . (３ ) The relation between L . hedysaroides( Pall .) Kitag . and L . hedysaroides ( Pall .) Kitag . var . subsericea ( Kom .) Kitag . was closer ; L . davurica ( Maxim .)Schindl . and L . potaninii Vass . Was also closer , but L . hedysaroides ( Pall .) Kitag . var . subsericea ( Kom .) Kitag . Waseven closer than L .hedysaroides ( Pall .) Kitag . and (４) Cluster analysis showed that the materials from the same species couldcluster together , but a few could not do the same . Relationships of inter‐species confirmed the traditional taxonomy of ３ speciesand １ variety belonging to Lespedez a Michx .ReferencesClewell A .F . １９６４ . The biology of the common native Lespedez as in southern Indiana .Brittonia , １６ :２０８‐２１９ .Hoveland C . S . and Donnelly E .D . １９８５ . The Lespedez as . In Forages , １２８‐１３５ .Zhang J Y . , ２００３ . Study on genetic diversity of １４ wild Lespedez a populations [D] . Gansu : Gansu A gricultural University .Zhao Yang . , ２００６ . Studies on genetic diversity of Lespedez a bicolor and the phylogentic relationship of Lespedez a Michxspecies [D] . Bei j ing : Bei j ing Foresty University .

Study on Genetic Diversity of Wild \u3ci\u3eLespedeza\u3c/i\u3e Germplasm Resources

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3465&amp;context=igc

Study on Genetic Diversity of Wild \u3ci\u3eLespedeza\u3c/i\u3e Germplasm Resources

Abstract

Similar works

Full text

Available Versions

University of Kentucky