Wei, Zhenwu

Yang, Zhanhua

Zhang, Lifang

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Development and Genetic Analysis of a Mapping Population on Medicago truncatula Zhenwu Wei Yangzhou University, China Zhanhua Yang Yangzhou University, China Lifang Zhang Yangzhou University, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/13-2/37 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 ]530　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems——— Domestication of Native Grasslands/Rangelands Plants for Regional UseDevelopment and genetic analysis of a mapping population on Medicago truncatulaZhenwu Wei , Zhanhua Y ang , L i f ang ZhangCollege o f A nimal Science & Technique , Y angz hou University , Y angz hou , 225009 , E‐mail :z wwei＠ yz u .edu .cnKey words : Medicago truncatula , annual medic , mapping population , genetic analysisIntroduction Medicago truncatula ( annual medic) has become a model system for legume biology . Comparative genetic mappingstudies can reveal similarities and differences in gene content and gene order between genera belonging to different taxa .Germplasm of annual medic was identified and screened for genetic mapping using agronomic characters and SSR markers . Ourobjective was to construct a mapping population of Medicago truncatula and perform genetic analysis on this population .Materials and methods A total of ２６ accessions of annual medic were planted in a randomized block design with three replicationsat Nanjing , China in spring of ２００７ . The sequences of SSRs primer pairs along with the SSR marker name , and linkage groupin soybean and M . truncatula were identified . Genetic diversity of each SSR locus was expressed by allelic richness and theSimpson genetic diversity index .Results Germplasm was screened by phenotype and genotype into two extreme polymorphic parental lines that were crossed andthen selfed to generate a mapping population for QTL analysis . We obtained ６１ polymorphic loci and ８８６ polymorphic alleles ,and a dendrogram was generated with the UPGMA clustering for ２６ annual medic by ６１ polymorphic markers ( Figure １ ) . Thelevels of phenotypic variation among ２６ annual medic accessions were similar to genetic diversity in DNA . A total of ３９ nuclearmicrosatellites ( nuSSR) and ３０ chloroplast microsatellites ( cpSSR) were used to screen ２６ accessions of annual medic . LineAFD , Caliph and J .５１ were selected as parents to obtain the F１ . The frequency distribution and fitted mixed distribution for１００‐pod weight and seed yield of the F２ population are shown in Figure ２ . Genetic mapping and QTL analysis will be conductedwith the F２ population . One of the targets was to focus on the seed yield of annual medic .Figure 1 Cluster Dendrogram o f ２６ annual medic based０n nuSSR and cpSSR .　 　Figure 2 Frequency distribution and f itted mixeddistribution f or １００‐pod weight and seed y ieldo f the W５１６０ × P５１６０ population .ReferencesCollard , B .C .Y . , Jahufer , M .Z .A . , Brouwer , J .B . , Pang , E .C .K . , ２００５ . An introduction to markers , quantitative traitloci ( QTL ) mapping and marker‐assisted selection for crop improvement : The basic concep ts . Euphytica １４２ , １６９‐１９６ .Seres ,A . ,Deák ,G . ,Tóth ,G . ,Aubert ,G . ,Burstin ,J . ,Ellis ,N . ,Kiss ,G .B . ,２００６ . Comparative mapping . Medicagotruncatula Handbook .

Development and Genetic Analysis of a Mapping Population on \u3ci\u3eMedicago truncatula\u3c/i\u3e

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Development and Genetic Analysis of a Mapping Population on \u3ci\u3eMedicago truncatula\u3c/i\u3e

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