Bai, Shiqie

Zhang, Jianbo

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Study on Gliadin Diversity Trait of Elymus nutans L. in the Northwestern Plateau of Sichuan Province Jianbo Zhang Guizhou Institute of Prataculture, China Shiqie Bai Sichuan Academy of Grassland Science, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/13-2/20 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　 瞯 ]513　 瞯Grasslands/Rangelands Production Systems——— Domestication of Native Grasslands/Rangelands Plants for Regional UseStudy on Gliadin diversity trait of Elymus nutans L . in the northwestern plateau of SichuanprovinceZHA NG Jian‐bo1 ,2 ,BA I Shi‐qie2 倡1 Guiz hou Institute o f Prataculture ,Dushan 558200 ,China ;2 Sichuan Grassland Science A cademy , Chengdu 611731 ,China ;First author ( z hang j ianber＠ 163 .com) ;倡 A uthor f or correspondenceKey words : drooping wild ryegrass , genetic diversity , Gliadin , cluster analysis , A‐PAGEIntroduction The Gliadin diversity was studied in this paper to contrast the Northwestern Plateau of Sichuan province accessionswith ５５ accessions , to discover the diversity traits of the Northwestern Plateau of Sichuan province accessions .Materials and methods Fifty‐five accessions of Elymus nutans L . were analyzed in this study , Gliadins were extracted accordingto ISTA摧s standard methods ( Draper １９８７ ) . All accessions were scored for presence or absence of the gliadin bands . Totalgenetic diversity was estimated using the Shannon‐Weaver ( SW) index ( H ) ( Wachira et al . １９９５ ) and Simpson index ( Nei ,１９７３ ) . The binary data were analyzed using a qualitative routine to generate Jaccard摧s genetic similarity coefficient ( GS) by theNTSYSpc ２ .１０ package ( Jaccard １９０８) . The similarity matrices were subjected to unweighted pair group method of arithmeticaverages ( UP‐AMGE) .Results The Gliadin diversity variation was less among accessions from the Northwest plateau of Sichuan province than amongthe other accessions based on the number of gliadin bands , Shannon index and Simpson index . But the number of commongliadin bands was more among accessions from the Northwest plateau of Sichuan province than among the other accessions .The dendrogram showed the gliadin diversity of Northwest plateau of Sichuan province accessions was related to altitude , butother meteorologic and geographical factors also influenced the gene differentiation . The dendrogram of the total group showed３ main clusters . The accessions from the Northwest plateau of Sichuan province were clustered in a group .Conclusions The accessions from the Northwestern Plateau of Sichuan Province had special bands from the Gliadin patterns .The phenomena that the total accessions had high a level of genetic diversity and the Northwestern Plateau of Sichuan Provinceaccessions had low genetic diversity was revealed by Shannon index and Simpson index .ReferencesDraper S .R .１９８７ .ISTA variety committee report of the working group for biochemical tests for cultivar identification １９８３‐１９８６ .Seed Sci .& Technol .１５ :４３１‐４３４ .Wachira F .N . , Waugh R . , Hachett C .A . and Powell W . １９９５ . Detection of genetic diversity in tea ( Camellia sinesis) usingRAPD marks . Genome ３８ :２０１‐２１０ .Nei M . １９７３ . Analysis of gene diversity in subdivided populations . Proc . Natl . Acad . Sci . USA ７０ :３３２１‐３３２３ .Jaccard P . １９０８ . Wonvelles recherches sur la distribution florale . Bull . Soc . V aud . Sci . Nat . ４４ :２２３‐２７０ .

Study on Gliadin Diversity Trait of \u3ci\u3eElymus nutans\u3c/i\u3e L. in the Northwestern Plateau of Sichuan Province

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3472&amp;context=igc

Study on Gliadin Diversity Trait of \u3ci\u3eElymus nutans\u3c/i\u3e L. in the Northwestern Plateau of Sichuan Province

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