Ban, Ting

Han, Peng

Liu, Xiang

Wang, Xiaojuan

Yang, Xiaoli

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress To Identify Alfalfa Cultivars Based on RAPD Markers Using Bulked and Individual DNA Xiaoli Yang Lanzhou University, China Ting Ban Lanzhou University, China Peng Han Lanzhou University, China Xiang Liu Lanzhou University, China Xiaojuan Wang Lanzhou University, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/12-2/35 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 ]416　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems——— Developing Improved PlantsTo identify alfalfa cultivars based on rapd markers using bulked and individual DNAX iaoli Y ang , T ing Ban , Peng H an , X iang L iu and X iao j uan W ang 倡College o f Pastoral A griculture Science and Technology , L anz hou University , L anz hou 730020 , China ,倡 Correspondence author : x iao j uanw ang＠ lz u .edu .cnKey words : alfalfa , cultivar , bulked DNA , individual DNA , RAPDIntroduction The genetic relationship and distance among alfalfa cultivars is of great interest for breeding programmers as wellas for cultivar identification . However , cultivated alfalfa ( Medicago sativ a L .) is tetraploidy out‐breeding species with a self‐incompatibility system , which causes a high degree of genetic variation among individuals within populations . The aim of thisstudy was to identify alfalfa cultivars based on RAPD markers using bulked and individual DNA , respectively .Materials and methods Sixteen alfalfa cultivars were used in this study . Genomic DNA was isolated using CTAB method . ２０ ,４０ and ６０ individual plants per population and bulked DNA were analyzed , respectively . A total of １０ RAPD primers were usedfor routine screening . The bands visualized on the gels were scored manually and each band in the RAPD profile was consideredas ０ ( absent) or １ ( presence) .Results and discussion Using bulked genomic DNA samples , four out of １０ primers detected the specific bands belong to ５cultivars respectively , which could be used to identify among alfalfa varieties . Figure １ ( a ) showed the patterns of a bulkedgenomic DNA samples from ２０ individuals per population using RAPD primer OPEL‐６ , two specific bands of cultivar�Longdong" (７８０ bp) and�Jindera" ( ９５０ bp) were appeared , and there were ６０％ individuals of cultivar �Longdong" to havethis specific band . The polymorphic bands did not increase with the numbers of the individuals bulked , which indicated that ２０was enough to identify �Longdong" and �Jindera" from other cultivars . To identify alfalfa cultivars using individual genomicDNA with １０ , ２０ , ４０ and ６０ plants per population , we found that it was difficult for the high variation within each population( Figure １ , b) . According to the results of this study , data based on RAPD markers were useful in identifying alfalfa cultivarvarieties using bulked genomic DNA samples from ２０ plants per population .Figure 1 RA PD patterns obtained f rom bulked genomic DNA samp les o f 16 cultiv ars ( a) and f rom 10indiv idual genomic DNA samp les o f cultiv ar �Longdong" (b) w ith p rimer OPEL‐6 .Acknowledgement This study was supported by National Basic Research Program of China (２００７CB１０８９０４ ) , Natural ScientificFoundation of Gansu Province ( No .３ZS０５１‐A２５‐０６６ ) , and Natural Science and Technology Program of Lanzhou University(５８２４０２ , ５８２４０３) .

To Identify Alfalfa Cultivars Based on RAPD Markers Using Bulked and Individual DNA

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To Identify Alfalfa Cultivars Based on RAPD Markers Using Bulked and Individual DNA

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