Cortisol is a versatile hormone that possesses both catabolic and anabolic functions in the body, such as increasing the blood glucose levels through gluconeogenesis and metabolizing carbohydrates, proteins, and fats.1234 Cortisol levels are controlled by communication of the hyptothalamic-pituitary-adrenal axix (HPA axis). Plasma concentration of cortisol adheres to a diurnal rhythm, meaning that cortisol levels are highest in the morning and taper off throughout the day

Granger, Amy

Henry, Allison

Injeti, Elisha R.

Lilliencrantz, Lauren

Smith, Amanda

Srnis, Paul

Van Schepen, William

Digital Commons

Effects of Physical Stress and Maturational Changes on Hypothalamic Pituitary Adrenal Axis Function Through Cortisol Analysis

Cedarville UniversityDigitalCommons@CedarvillePharmacy and Nursing Student Research and Evidence-Based Medicine Poster Session11-2012Effects of Physical Stress and Maturational Changeson Hypothalamic Pituitary Adrenal Axis FunctionThrough Cortisol AnalysisAmy GrangerCedarville University, amygranger@cedarville.eduAllison HenryCedarville University, allisonhenry@cedarville.eduLauren LilliencrantzCedarville University, llillien@cedarville.eduAmanda SmithCedarville University, amandagsmith@cedarville.eduPaul SrnisCedarville University, psrnis@cedarville.eduSee next page for additional authorsFollow this and additional works at: http://digitalcommons.cedarville.edu/pharmacy_nursing_poster_sessionPart of the Pharmacy and Pharmaceutical Sciences CommonsThis Poster Session is brought to you for free and open access byDigitalCommons@Cedarville, a service of the Centennial Library. It hasbeen accepted for inclusion in Pharmacy and Nursing Student Researchand Evidence-Based Medicine Poster Session by an authorizedadministrator of DigitalCommons@Cedarville. For more information,please contact digitalcommons@cedarville.edu.Recommended CitationGranger, Amy; Henry, Allison; Lilliencrantz, Lauren; Smith, Amanda; Srnis, Paul; Van Schepen, William; and Injeti, Elisha R., "Effectsof Physical Stress and Maturational Changes on Hypothalamic Pituitary Adrenal Axis Function Through Cortisol Analysis" (2012).Pharmacy and Nursing Student Research and Evidence-Based Medicine Poster Session. 3.http://digitalcommons.cedarville.edu/pharmacy_nursing_poster_session/3AuthorsAmy Granger, Allison Henry, Lauren Lilliencrantz, Amanda Smith, Paul Srnis, William Van Schepen, andElisha R. InjetiThis poster session is available at DigitalCommons@Cedarville: http://digitalcommons.cedarville.edu/pharmacy_nursing_poster_session/3* Effects of physical stress and maturational changes on hypothalamic pituitary  adrenal axis function through cortisol analysis Amy Granger, Allison Henry, Lauren Lilliencrantz, Amanda Smith,  Paul Srnis, William Van Schepen, Elisha Injeti, PhD  Cedarville University School of Pharmacy STATEMENT OF THE PROBLEM • The purpose of this study is to investigate how cortisol levels change in response to stress in adolescents and geriatrics • The goals of this study are to establish baseline diurnal cortisol concentration, establish the correlation between physical stressors and cortisol distribution, and to analyze kinetic parameters associated with cortisol distribution  OBJECTIVES Using the procedures stated in the methods. The concentrations of unbound cortisol will be indirectly calculated using a 4-parameter logistic nonlinear regression model that will be put into semilog form.  T tests will then be computed using SPSS to analyse the means of the different subgroups.   PROPOSED METHODS PROPOSED ANALAYSES 1. Cook CJ, Crewther BT, Smith AA. Comparison of baseline free testosterone and cortisol concentrations between elite and non-elite female athletes. Am J Hum Biol. 2012. 2. Passelergue P, Robert A, Lac G. Salivary cortisol and testosterone variations during an official and a simulated weight-lifting competition. Int J Sports Med. 1995;16:298-303. 3. Snegovskaya V, Vira A. Elevation of cortisol and growth hormone levels in the course of further improvement of performance capacity in trained rowers. Int J Sports Med. 1993;14:202-206. 4. Hoehn K ME. Human anatomy & physiology. San Fransico: Benjamin Cummings; 2010. 5. Gunnar MR, Fisher PA, Early Experience, Stress,and Prevention Network. Bringing basic research on early experience and stress neurobiology to bear on preventive interventions for neglected and maltreated children. Dev Psychopathol. 2006;18(3):651-677. 6. Kudielka BM, Buske-Kirschbaum A, Hellhammer DH, Kirschbaum C. HPA axis responses to laboratory psychosocial stress in healthy elderly adults, younger adults, and children: Impact of age and gender. Psychoneuroendocrinology. 2004;29(1):83-98. 7. http://www.encephalos.gr/48-3-07e.htm • Dr. Elisha Injeti, Project Advisor ACKNOWLEDGEMENTS REFERENCES Study Design:  • Prospective, exploratory design  Sample:  • Control group does not get physical stressor • Treatment group does get  physical stressor  • Control and Treatment groups are subdivided   into Adult and Geriatric • Further broken down into male and female      within Adult and Geriatric  Data Collection: • Establish cortisol kinetics by taking saliva samples every 15-20 minutes from 6-9 AM • Establish baseline diurnal cortisol levels by taking 6 total saliva samples every 2 hours from 6 AM to 9 PM • Remeasure cortisol kinetics and diurnal rhythms after 1 month and again after 9-12 months    Measurement:  • Measuring free cortisol levels from collected saliva samples using Salimetrics Salivary Cortisol Enzyme Immunoassay  Kit   HYPOTHESES • Measurements will be taken over the course of a year • Once all the samples are collected we will begin carrying out the assays to measure free cortisol levels • Based on results of the assays we will carry out data analysis to determine the significance of our study • The entire will be completed within 2 and half years PROJECT TIMELINE • Participants may not consistently follow exercising regimen • Participants may exercise in the afternoon rather than the morning • Participants may drop out before we can collect long-term data  • Limited generalization because of the convenience sampling and small sample size LIMITATIONS • The results of this study will provide foundational research that will lead to more personalized therapies for patients with altered HPA axis function    FUTURE DIRECTIONS    Cortisol is a versatile hormone that possesses both catabolic and anabolic functions in the body, such as increasing the blood glucose levels through gluconeogenesis and metabolizing carbohydrates, proteins, and fats.1234 Cortisol levels are controlled by communication of the hyptothalamic-pituitary-adrenal axix (HPA axis). Plasma concentration of cortisol adheres to a diurnal rhythm, meaning that cortisol levels are highest in the morning and taper off throughout the day.      Free cortisol levels in the body are most commonly measured by salivary assays.5 External factors, such as stress, initiate the process that produces cortisol in the body. Stressors can be physical, psychosocial, or physiological/pathological in nature. Exercise will the be physical stressor that will be evaluated in this study. Currently, there is a shortage of studies investigating the impact of stress on HPA axis response and cortisol levels.6 The insight gained from this study will lend greater understanding to the HPA axis function in response to stress and maturation. As greater understanding is achieved, this will lead to personalized medication profiles and therapeutic dosing regimens for patients with cortisol regulation disorders. 7 • Cortisol levels will increase in patients that undergo physical stress, such as exercise • Cortisol levels will be higher in the adult population than in the geriatric population 

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Van Schepen, William John

https://digitalcommons.cedarville.edu/cgi/viewcontent.cgi?article=1002&context=pharmacy_nursing_poster_session

Effects of Physical Stress and Maturational Changes on Hypothalamic Pituitary Adrenal Axis Function Through Cortisol Analysis

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