Bai, Xue

Fang, Hengtong

Hao, Zhuo

Shen, Jinglin

Shi, Xudong

Tang, Hongyu

Wu, Xia

Zhang, Jing

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Differential Proteomics Analysis of Leaves from Hordeum Brevisublatum under Salt Stress Jinglin Shen Jilin University, China Xia Wu Jilin University, China Hongyu Tang Jilin University, China Jing Zhang Jilin University, China Hengtong Fang Jilin University, China See next page for additional authors Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/12-2/52 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. Presenter Information Jinglin Shen, Xia Wu, Hongyu Tang, Jing Zhang, Hengtong Fang, Xudong Shi, Xue Bai, and Zhuo Hao This event is available at UKnowledge: https://uknowledge.uky.edu/igc/21/12-2/52 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　 瞯 ]433　 瞯Grasslands/Rangelands Production Systems——— Developing Improved PlantsDifferential proteomics analysis of leaves from Hordeum Brevisublatum under salt stressShen Jinglin , W u X ia , Tang Hongyu , Zhang Jing , Fang Hengtong , Shi X udong , Bai Xue and H ao ZhuoThe College o f A nimal Husbandry and Veterinary Medicine Jilin University , Jilin Changchun ,China 130062 ,E‐mail :shenj inglinshen＠ yahoo .com .cnKey words : proteomics , salt stress , Hordeum brev isubulatium ( T rin) Link , ribulose‐１ ,５‐bisphosphate carboxylase/ oxygenasesmall subunit , ProteomicsIntroduction Proteomics provide a complementary and potentially more comprehensive approach to the analysis of signalingmechanisms . Changes in protein profiles during signaling events can be monitored using two‐dimensional ( ２D ) gelelectrophoresis or related techniques , detecting alterations in expression levels . Hordeum brev isubulatium ( T rin .) Link , anexcellent forage with high nutritional value and yield , has great economic value . On the basis of previous research onphysiological and biochemical features of H . brev isubulatiu . , its salt‐resistant mutant , and the salt‐tolerant molecularmechanism in our Lab . We designed a study with the objective to identify proteins differentially expressed in leaves from H .brev isubulatium .under salt stress using two dimensional electrophoresis combined with mass spectrometry to further ourunderstanding of the action mechanism of H . brev isublatum under salt stress .Materials and methods The Protein sample was collected from the leaf of the H . brev isubulatium . grown in the lab under saltstress . We construct and modified the two dimensional electrophoresis method according to the material . Two‐dimensional gelelectrophoresis which is important in proteomics was improved . Some important steps , such as sample preparation , isoelectricfocusing , balance , and dyeing were improved . For sample preparation , the tissue sample was ground in liquid nitrogen and thenhomogenized in the lysis buffer ( ８M urea , ２M thiourea , ５０mM DTT , ０ .５％ carrier amphorlyte and １mM PMSF ) , thencentrifuged at １５０００ rpm for １ h at room temperature . The sample was carefully recovered , avoiding the unhomogenizedmaterial at the bottom of the centrifuge tube . For the preparation of gel staining , we tried the�blue silver" method to improvethe resolution of the two dimensional electrophoresis map .Results Using two dimensional electrophoresis and Peptide Mass Fingerprint ( PMF ) analysis , the differential expressedprotein : ribulose‐１ ,５‐bisphosphate carboxylase/ oxygenase small subunit was identified . It implied that a ribulose‐１ , ５‐bisphosphate carboxylase/ oxygenase small subunit may be involved in the salt stress of H . brev isubulatium . .Conclusions The express of the protein ribulose‐１ , ５‐bisphosphate carboxylase/ oxygenase small subunit was reducingsignificantly in the salt stress group than it was in control group . It indicated that a ribulose‐１ ,５‐bisphosphate carboxylase/oxygenase small subunit may played an important part in the salt stress of H . brev isubulatium .ReferencesGorham J . １９９４ . Salt tolerance in the T riticae : K ＋ / Na ＋ discrimination in some perennial wheatgrasses and their amphiploidswith wheat . J . Ex p . Bol . , ４５ : ４４１‐４４７ .Daniels MJ . The plasma membrane of Arabidopsis thaliana contains a mercury‐insensitive aquaporin that is a homolog of thetonoplast water channel protein T IP . Plant Physiol , １０６ : １３２５‐１３３３ .Liu J . Zhu J . K . １９９８ . A calcium sensor homolog required for plant salt tolerance . Science , ２８０(５３７１) : １９４３‐１９４５ .

Differential Proteomics Analysis of Leaves from \u3ci\u3eHordeum Brevisublatum\u3c/i\u3e under Salt Stress

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3357&amp;context=igc

Differential Proteomics Analysis of Leaves from \u3ci\u3eHordeum Brevisublatum\u3c/i\u3e under Salt Stress

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