Bushman, B. Shaun

Larson, S. R.

Mott, I. W.

Wang, Richard R. C.

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Development of Genomic Tools for Wheatgrass and Wildrye Improvement Richard R. C. Wang U.S. Department of Agriculture B. Shaun Bushman U.S. Department of Agriculture S. R. Larson U.S. Department of Agriculture I. W. Mott U.S. Department of Agriculture Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/12-1/4 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　 瞯 ]335　 瞯Grasslands/Rangelands Production Systems——— Developing Improved PlantsDevelopment of genomic tools for wheatgrass and wildrye improvementR . R .‐C . W ang , B . S . Bushman , S . R . L arson , I . W . MottUSDA‐ARS , Forage & Range Research L aboratory , Logan , UT 84322‐6300 , USA , Richard .W ang＠ ars .usda .govKey words : bacterial artificial chromosome ( BAC ) ,expressed sequence tag ( EST ) ,mapping ,quantitative trait loci ( QTL ) ,simple sequence repeat ( SSR)Introduction Wheatgrass and wildrye grasses are some of the most important grasses in the temperate regions of the world( Asay and Jensen , １９９６a , １９９６b) . Cultivars of wheatgrass and wildrye grasses are mainly used as forage crops on rangelandand pastures in North America . Their drought and/ or salinity tolerance make them suitable plant materials for revegetation andland reclamation . As members of the T riticeae tribe , they are related to and have been hybridized with cultivated cereal cropsincluding wheat ( T riticum spp .) , barley ( Hordeum spp .) , and rye ( Secale cereale L .) as genetic sources for pest ( diseaseand insect) resistance , salinity tolerance , and other traits .Materials and methods To facilitate breeding of wheatgrass and wildrye grasses , we have developed expressed sequence tag( EST ) libraries for bluebunch wheatgrass ( Pseudoroegneria sp icata ) , Elymus , and Leymus as well as bacterial artificialchromosome (BAC) libraries for Leymus in cooperation with two collaborating universities ( Bushman et al .２００８ ; Larson et al .２００７ ) . SSR markers in the T riticeae libraries were developed , tested for amplification in a suite of species , and used for geneticmapping on interspecific mapping populations (Wu et al . , ２００３ ) . Overgo primers for screening BAC libraries were designedand tested . BAC clones containing target gene were identified , partially sequenced and mapped .Results and discussion Three EST libraries have been constructed using various tissues/ organs and treatments of P . sp icata , L .cinerius × L . triticoides , and E . w awawaiensis × E . lanceolatus ( Table １ ) . We are using the EST library at this time forSSR and STS marker development , comparative genomics , expression studies and functional genomics . Two BAC libraries forthe L . cinereus × L . triticoides hybrid with two restriction enzymes , BamHI and MboI consist of ４０５ ,８８８ clones arrayed in １ ,０５７ ３８４‐well microplates and have an average insert size of １５０ .５ kb . The combined libraries are equivalent to approximately ６ .１ × haploid genomes . The BAC libraries had been screened with ２５ Overgo primer sets covering ２３ genes , for which about １６０positive BAC clones were identified . BACs containing the orthologous Lax rice gene were mapped on group ３ chromosomes ofthe Ns and Xm genomes in the allotetraploid Leymus hybrid that are homoeologous to rice chromosome １ . Selected BAC cloneswill be sequenced , aligned , and used for physical mapping . These genomic resources will lead to the development of molecularmarkers ( SSR , STS , and SNP) to assist breeding efforts on wheatgrass and wildrye species .Table 1 EST libraries constructed and used .Library Clones ％ BLAST hits Unigenes Markers tested Informative MappedPseudoroegneria １３ ,７７７ 3９３ 妹８ ,７８０ 缮４０８ �１０１ j４７ 剟Leymus １４ ,３９３ 3９１ 妹１１ ,２８１ 鼢１ ,６５６ Y７４１ j２００ 膊Elymus ８ ,７５８  ９１ 妹７ ,２１２ 缮８０９ �１０７ j５６ 剟ReferencesAsay , K . H . , Jensen , K . B . １９９６a . Wheatgrasses . In LE Moser , DR Buxton , MD Casler , Cool‐Season Forage Grasses ,Agronomy Monograph no . ３４ , A SA‐CSSA‐SSSA , Madison , W I . , Chapter ２２ , ６９１‐７２４ .Asay , K . H . , Jensen , K . B . １９９６b . Wildryes . In LE Moser , DR Buxton , MD Casler , Cool‐Season Forage G rasses ,Agronomy Monograph no . ３４ , A SA‐CSSA‐SSSA , Madison , W I . , Chapter ２３ , ７２５‐７４８ .Bushman , B . S . , Larson , S . R . , Mott , I . W . , Wang , R . R .‐C . , Liu , L . , Hernandez , A . , Kim ,W . R . , Thimmapuram ,J . , Mikel , M . A . ２００８ . Cross species utility and comparative genomic use of SSR markers derived from perennial T riticeaegrasses . Plant and A nimal Genome Meeting XV I , San Diego , CA , Jan １２‐１６ , ２００８ .Larson , S . R . , Scheuring , C . , Bushman , B . S . , Mott , I . W . , Dong , J . J . , Zhang , Y . , Zhang , X . , Zhang , H . B . ,Wang , R . R .‐C . ２００７ . Construction of a BAC library of the Leymus cinereus × L . triticoides hybrid . In : A nnualMeetings A bstracts , A SA‐CSSA‐SSSA , Madison , W I . CDROM .Wu , X . L . , Larson , S . R . , Hu , Z . M . , Palazzo , A . J . , Jones , T . A . , Wang , R . R .‐C . , Jensen , K . B . , Chatterton , N .J . ２００３ . Molecular genetic linkage maps for allotetraploid Leymus ( T riticeae) . Genome , ４６ , ６２７‐６４６ .

Development of Genomic Tools for Wheatgrass and Wildrye Improvement

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Development of Genomic Tools for Wheatgrass and Wildrye Improvement

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