Deng, Chengjun

Li, Cong

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Effect of NTHK1 Gene to Transgenic Alfalfa Leaf Enlargement Cong Li Chinese Academy of Agricultural Sciences, China Chengjun Deng Chinese Academy of Agricultural Sciences, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/12-1/41 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 ]372　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems——— Developing Improved PlantsEffect of NTHK1 gene to transgenic alfalfa leaf enlargementCong L i , Cheng‐j un DengForage Genetics and Breeding L ab , Institute o f A nimal Science , CA A S , Bei j ing , 100094Key words :NTHK１ ,gene ,alfalfa ,transgenic plant ,leaf enlargement ,effect analysisIntroduction Living cells respond rapidly to the surrounding stimuli to adapt to changes in their environment . One of themechanisms of these stimuli is known as the �two‐component�regulatory system . Ethylene receptors sense ethylene andregulate downstream signaling events . Tobacco ethylene receptor NT HK1 , has been found to function in plant grow th andsalt‐stress response . The NT HK1 cDNA has a length of ２７３２ bp and encodes a protein of ７６２ amino acids with a molecularmass of ８５ .３ kDa and a pI of ６ .７ . NTHK１ contains transmembrane domains , a GAF domain , a kinase domain ( KD) and areceiver domain ( RD ) ( Figure １ ) . The transmembrane domains plus GAF domain may have a role in promoting leaf cellgrow th , and the kinase domain may strengthen this role ( Zhou , et al . , ２００６) .Figure 1 Structure o f N T H K1 .Material and methods The plasmids of the NT HK1 gene were introduced into alfalfa by A grobacterium mediated transformationand got the transgenic plants . Sixty four transferred plants were obtained , and checked １９ plants of them , and ２ positive plantswere obtained by PCR and RT‐PCR analyses .Results and conclusion The results demonstrated that the NT HK1 gene has been integrated into alfalfa genome . And the leavesof transgenic alfalfa are much larger than for control . By measure and calculation , the leaves�area of transgenic plant is twoand half times of the control . This result shows the gene function of leaves�enlargement ( Figure ２ , ３) .Figure 2 Leaf o f transgenic al f al f a is largerthan o f control .Note : the le f t is transgenic al f al f a ; the right is CK　 　Figure 3 Leaf o f transgenic al f al f a is largerthan o f the control .Note : the le f t is transgenic al f al f a ; the right is CKReferenceH . L . Zhou , W . H . Cao , Y . R . Cao , J . Liu , Y . J . Hao , J . S . Zhang , S . Y . Chen , ２００６ . Roles of ethylene receptorNTHK１ domains in plant grow th , stress response and protein phosphorylation . FEBS ５８０ : １２３９‐１２５０

Effect of NTHK1 Gene to Transgenic Alfalfa Leaf Enlargement

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Effect of NTHK1 Gene to Transgenic Alfalfa Leaf Enlargement

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