Fan, Yan

Li, Fang

Ma, Xiao

Zhang, Xinquan

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Genetic Diversity of Hemarthria compressa Germplasm in Southwest China Detected by ISSR Markers Fang Li Sichuan Agricultural University, China Yan Fan Sichuan Agricultural University, China Xinquan Zhang Sichuan Agricultural University, China Xiao Ma Sichuan Agricultural University, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/12-1/11 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 ]342　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems——— Developing Improved PlantsGenetic diversity of Hemarthria compressa germplasm in southwest China detected by ISSRmarkersFang L i1 , Y an Fan1 , 2 , X inquan Zhang1 倡 ,X iao Ma11 Department o f G rassland Science , Sichuan A gricultural University , Y aan 625014 , China ,E‐mail : z hangxq＠ sicau .edu .cn . 2 Chongqing Municipal Institute o f A nimal Husbandry ,Chongqing 400039 , ChinaKey words : Hemarthria compressa ,ISSR markers ,genetic diversity ,southwest ChinaIntroduction Hemarthria comp ressa is one of the most important and widely utilized forages in south China , owning to its longgrowing season , quick growing habit , good ratooning ability , high yielding and good capability of adaptation to hot or droughtconditions . In this study , Inter‐simple sequence repeat ( ISSR) molecular markers were applied to detect genetic diversity oftwenty‐eight Hemarthria comp ressa accessions from Southwest ChinaMaterials and methods Total DNA was extracted using a modified CTAB method . Thirteen primers were selected for the ISSRanalysis as they could produce reproducible and clear bands . PCR amplification reactions were carried out in ２０ μl volume ,containing １０ × PCR Buffer ２ μL , MgCl２ １ .３ μL(２５ mm/L ) ,dNTP ２ μL (２ .５ mm/L ) ,１ .５ μL ( １０ μm/ μL ) primers , ０ .２ μL (５U / μL) Taq DNA polymerase and ６０ ng of template DNA . PCR amplification was performed as follows : initially ５ min at ９４ ℃ ;４５ cycles of ４５ s at ９４ ℃ , ６０ s at ５２ ℃ and ９０ s at ７２ ℃ ; and a finally ７‐min�extension at ７２ ℃ . Only bands that could beunambiguously scored across all the sampled populations were used in this study . ISSR amplified fragments , with the samemobility to the molecular weight ( bp) , were scored manually for band presence ( １ ) or absence ( ０ ) . The resulting presence/absence data matrix was analyzed using NTSYS‐pc , version ２ .１０ .Results The following results were obtained : ( １ ) Thirteen primers produced １１０ polymorphic bands , the percentage ofpolymorphic bands on average was ８４ .２％ . The Nei摧s genetic similarity coefficient of the tested accessions ranged from ０ .４７ to０ .９８ . These results suggested that there was rich genetic diversity among the resources of H . comp ressa tested . (２) Accordingto both UPGMA dendrogram and principal component analysis , twenty‐eight accessions were clustered into two groups , withaccessions from the same origin frequently clustered into one group . The findings implied the associations among the resources ,geographical and ecological environment . Otherwise , the clones selected from the�Guangyi" whipgrass clustered into the samegroup showing the reliability of ISSR markers applied in H . comp ressa . ( comment : re‐w rite or rephrase this sentence since it isnot clear !) Moreover ,the two groups were divided into four sub‐groups , with the accessions possessing similar morphologicalcharacters were in the same sub‐group , consistent with the morphological cluster( Chen , et al . , ２００５) .Conclusions The average percentage of polymorphic bands was ８４ .２％ in the study . ISSR appears to be a powerful tool forassessing the genetic diversity in H . comp ressa germplasm . But a systematic understanding of the genetic diversity of H .comp ressa in China requires more comprehensive collection throughout its distribution .ReferencesYongxia CHEN , Xinquan ZHANG , Chunhua YANG . et al . , ２００５ . The study on morphologic diversity of wild Hemarthriacompressa from Southwest China . Grassland o f China ２７(１) :７７‐７９ .Acknowledgment This research was supported by National Basic Research Program ( ９７３ Program ) in China ( No .２００７CB１０８９０７ ) and the Natural Science Foundation Program ( No . ２００６BA１０２２ ) , Science and Technology Bureau ofChongqing City , PRC .

Genetic Diversity of \u3ci\u3eHemarthria compressa\u3c/i\u3e Germplasm in Southwest China Detected by ISSR Markers

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3266&amp;context=igc

Genetic Diversity of \u3ci\u3eHemarthria compressa\u3c/i\u3e Germplasm in Southwest China Detected by ISSR Markers

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