Choi, Gi-jun

Ji, Hee-chung

Kim, Ki-yong

Kwak, Sang‐soo

Lee, Byung‐hyun

Lee, Joung-kyong

Lee, Sang-hoon

Lim, Keun-bal

Seo, Sung

Seong, Byung-ryul

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Field Performance of Transgenic Tall Fescue (Festuca arundinacea Schreb.) with Enhanced Tolerance to Environmental Stress Ki-yong Kim National Institute of Animal Science, South Korea Sang-hoon Lee National Institute of Animal Science, South Korea Gi-jun Choi National Institute of Animal Science, South Korea Hee-chung Ji National Institute of Animal Science, South Korea Joung-kyong Lee National Institute of Animal Science, South Korea See next page for additional authors Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/12-1/39 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. Presenter Information Ki-yong Kim, Sang-hoon Lee, Gi-jun Choi, Hee-chung Ji, Joung-kyong Lee, Keun-bal Lim, Byung-ryul Seong, Sung Seo, Byung‐hyun Lee, and Sang‐soo Kwak This event is available at UKnowledge: https://uknowledge.uky.edu/igc/21/12-1/39 瞯 ]370　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems——— Developing Improved PlantsField performance of Transgenic tall fescue ( Festuca arundinacea Schreb .) with enhancedtolerance to environmental stressK i‐Yong K im1 , Sang‐Hoon Lee1 , Gi Jun Choi1 , Hee Chung Ji1 , Joung K yong Lee1 ,K eun Bal L im1 , Byung Ryul Seong1 , Sung Seo1 , Byung‐Hyun Lee2 , Sang‐Soo K wak31 National Institute o f A nimal Science , RDA , Cheonan 330‐801 , Korea ; 2 Gyeongsang Natl . Univ . , Jinj u 660‐701 , Korea ;3 Korea Research Institute o f Bioscience & Biotechnology , Dae jeon 305‐806 , KoreaKey words :AtNDPK gene ,tall fescue ,environmental stress ,agrobacterium ,transgenic plantIntroduction NDP kinases ( NDPKs ) are multifunctional proteins that regulate a variety of eukaryotic cellular activities ,including cell proliferation , development , and differentiation . Arabidopsis NDPK２ ( AtNDPK２ ) is a key signaling componentthat regulates cellular redox state and is known to enhance multiple stress tolerance when over‐expressed in Arabidopsis plant .SWPA２ promoter had previously been found to encode an anionic POD which was highly expressed in response to oxidativestress .Materrials and methods The expression vector , pSN‐H was used for genetic transformation of tall fescue . Genomic DNA wasextracted from tall fescue plants . Af ter acclimatization in green house conditions , transgenic and control plants were transferredto a GMO isolated field . During summer in both ２００６ and ２００７ , field‐grown control and SN‐transgenic tall fescue plants werenaturally subjected to various environmental stresses including high temperature , water logging , and severe drought .Results and discussion Genomic DNA PCR and Southern blot analysis confirmed the incorporation of AtNDPK into the tallfescue genome . Under field condition , SN‐transgenic tall fescue plants performaned better than the control plants exposed to allthe environmental stresses . These results suggest that the response of SN‐transgenic tall fescue plants may be due to inductionof other stress responsive genes . An interesting result was observed in our investigation . We found that under normal growingcondition the SWPA２ : : AtNDPK２ overexpressing transgenic tall fescue plants showed vigorous healthy grow th activitycompared to the control plants at ４ months after sowing .Conclusions AtNDPK２ gene was introduced into tall fescue plants and Genomic DNA PCR and Southern blot analysis confirmedthe incorporation of AtNDPK２ into the tall fescue genome . Under filed condition , transgenic tall fescue plants showed betterperformance than the control plants exposed to environmental stresses including high temperature , water logging and severedrought stress .ReferenceMoon , H . , Lee , B . , Choi , G . , Shin , D . , Prasad , D .T . , Lee , O . , Kwak , S .S . , Kim , O .H . , Nam , J . , Bahk , J . , Hong ,J .C . , Lee , S .Y . , Cho , M .J . , Lim , C .O . and Yun , D .J . ２００３ . NDP kinase ２ interacts with two oxidative stress‐activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants . Proc . Natl .A cad . Sci . USA . １００ :３５８‐３６３ .Acknowledgement This work was supported by a grant ( Code ２００７０３０１０３４０１５) f rom BioGreen ２１ Program , RDA , Korea .

Field Performance of Transgenic Tall Fescue (\u3ci\u3eFestuca arundinacea\u3c/i\u3e Schreb.) with Enhanced Tolerance to Environmental Stress

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3294&amp;context=igc

Field Performance of Transgenic Tall Fescue (\u3ci\u3eFestuca arundinacea\u3c/i\u3e Schreb.) with Enhanced Tolerance to Environmental Stress

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