Andueza, D.

Ballet, J.

Dian, P. H. M.

Jailler, R.

Jestin, M.

Pourrat, J.

Prache, S.

Prado, I. N.

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Diet Authentication in Lamb Meat Using Spectroscopic Methods P. H. M. Dian INRA, France D. Andueza INRA, France J. Ballet INRA, France R. Jailler INRA, France M. Jestin INRA, France See next page for additional authors Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/10-1/34 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. Presenter Information P. H. M. Dian, D. Andueza, J. Ballet, R. Jailler, M. Jestin, J. Pourrat, I. N. Prado, and S. Prache This event is available at UKnowledge: https://uknowledge.uky.edu/igc/21/10-1/34 瞯 ]222　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems——— Integration of Crops , Forage and Forest SystemsDiet authentication in lamb meat using spectroscopic methodsP . H . M . Dian1 , D . A nduez a1 , J . Ballet2 , R . Jailler 1 , M . Jestin1 , J . Pourrat1 , I . N . p rado3 , S . Prache11 INRA , UR1213 Herbivores , S ite de Theix , F‐63122 Saint‐Genès‐Champanelle , France ;2 INRA , ,UE1153 Monts Dore , LeRoc , F‐63210 Orciv al ; 3 UEM , A v . Colombo 5790 , 87020‐900 , Bloco 32‐Sala 3 , Maringá‐PR , Braz ilCorresponding author : S . Prache . E‐mail : p rache＠ clermont .inra .f rKey words : diet authentication ,NIR spectroscopy ,reflectance ,carcass ,pasture‐feedingIntroduction The sensory and nutritional properties of the meat from pasture‐fed lambs differ from those of grain‐fed lambs .Furthermore , consumers are demanding clear information on the food supplied to animals . It is therefore important to be able todiscriminate between products obtained in different production systems , in particular pasture‐feeding v . stall‐feeding . Thisstudy evaluated the potential of three spectral method to discriminate pasture‐fed ( P ) v . stall‐fed ( S ) lamb carcasses : i )quantifying the light absorption in the ４５０‐５１０ nm area of the visible reflectance spectrum ( method １ , M１ ) as previouslyproposed by Prache and Theriez ( １９９９ ) , ii ) using the overall optical information of the visible reflectance spectrum ( i .e .between ４００‐７００ nm) (method ２ , M２) , as proposed by Dian et al (２００７ ) and iii) using visible and near infrared spectroscopy( NIRS) between ４００ and ２５００ nm ( method ３ , M３ ) . The first two methods used a portable spectrophotometer , the latter anon‐portable laboratory spectrophotometer .Materials and methods A total of ２５９ ( １２０ P and １３９ S ) Limousine lambs were used . Pasture‐fed lambs grazed a permanentpasture that was maintained at a leafy , green vegetative stage , and offered ad libitum ; they received no supplementation atpasture . Body weight of P lambs when turning out to pasture and at slaughter was ９ .４ kg ( s .d . ２ .２３) kg and ３３ .３ ( s .d . ２ .９１ ) kg respectively . S lambs were fed indoors on an ad libitum diet of commercial concentrate and hay until slaughter at a meanbody weight of ３３ .９ ( s .d . ３ .７１) kg . The visible reflectance spectrum of perirenal fat was measured at ２４h post mortem , withthe optical data taken every １０ nm . A sample of perirenal fat was then taken at ２４h post mortem , packed in aluminum foil , thenin a vacuum and stored in a conventional freezer a‐２０ ℃ until NIRS analysis . The visible‐near infrared spectrum was takenbetween ４００‐２５００ nm , with the optical data taken every ２ nm . In M１ , the fat reflectance spectrum data were used atwavelengths between ４５０ and ５１０ nm to calculate an index quantifying light absorption by carotenoid pigments . In methods ２and ３ , a multivariate analysis was performed over the full set of fat reflectance data . The raw reflectance spectra of perirenal fatrepresenting the two feeding treatments were submitted to discriminant analysis using the PLS‐DA method . Previously ,principal component analysis was performed and the mean reflectance spectrum from each feeding treatment was rankedaccording to the Mahalanobis distance ( H) to the average reflectance spectrum in order to detect outliers samples ( H ＞ ３) . Nooutliers were found . The models were tested by a cross‐validation procedure . The proportion of correctly classified lambs wasanalysed using the CATMOD procedure of SAS using a two‐factor model ( feeding treatment and method ) with repeatedmeasures on the last factor .Results and discussion The proportion of correctly classified P lambs was ８９ .２ , ９０ .８ and ９７ .５ for M１ , M２ and M３ ; theperformance of M３ was higher than that of M１ and M２ ( P ＜ ０ .０１ ) , the performance of the latter being not significantlydifferent . The proportion of correctly classified S lambs was ９８ .６ , ９８ .６ and ９７ .８ for M１ , M２ and M３ , these values being notsignificantly different .Conclusions M３ yielded a higher proportion of correctly classified lambs compared with M１ and M２ ( P ＜ ０ .００１ ) . Theproportion of correctly classified lambs using M３ was ９７ .８％ and ９７ .５％ for stall‐fed and pasture‐fed lambs respectively .ReferencesDian , P .H .M . , Andueza , D . , Barbosa , C .P . , Amoureux , S . , Jestin , M . , Carvalho , P .C .F . , Prado , I .N , Prache , S . ,２００７ . Methodological developments in the use of visible reflectance spectroscopy for discriminating pasture‐fed fromconcentrate‐fed lamb carcasses . A nimal １ (８) , １１９８‐１２０８ .Prache , S . , Theriez , M . , １９９９ . T raceability of lamb production systems : carotenoids in plasma and adipose tissue . A nimalScience ６９ , ２９‐３６ .

Diet Authentication in Lamb Meat Using Spectroscopic Methods

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Diet Authentication in Lamb Meat Using Spectroscopic Methods

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