Chen, Youguang

Guo, Zhenfei

Lu, Shaoyun

Niu, Yuejing

Tang, Huashan

Xie, Guozheng

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Breeding for Cold‐Tolerant Lines of Stylosanthes guianensis in Southern China Zhenfei Guo South China Agricultural University, China Huashan Tang South China Agricultural University, China Guozheng Xie South China Agricultural University, China Yuejing Niu South China Agricultural University, China Youguang Chen South China Agricultural University, China See next page for additional authors Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/12-1/35 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. Presenter Information Zhenfei Guo, Huashan Tang, Guozheng Xie, Yuejing Niu, Youguang Chen, and Shaoyun Lu This event is available at UKnowledge: https://uknowledge.uky.edu/igc/21/12-1/35 瞯 ]366　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems——— Developing Improved PlantsBreeding for cold‐tolerant lines of Stylosanthes guianensis in southern ChinaZhen f ei Guo 倡 , H uashan Tang , Guoz heng X ie , N iu Yue j ing , Youguang Chen , Shaoyun LuBiotechnology L aboratory f or Forages and Tur f grass , College o f L i f e Science , South China A gricultural University ,Guangz hou 510642 , China . 倡 E‐mail : z h f guo＠ scau .edu .cnKey words : breeding ,cold tolerance ,Sty losanthes guianensisS ty losanthes guianensis is an important forage legume with high yield and quality , acidity tolerance , and excellent adaptation tolow fertility soil in tropical and subtropical areas worldwide . It has also been used as a cover crop in agricultural farming ,grown as green manure , cut for either fresh fodder or hay , and sown to rehabilitate the soil fertility of mine site . The lowtemperature in winter is a key factor limiting its grow th and production in the subtropical regions . In order to induce and selectchilly‐tolerant mutants of S . guianensis , a total of fif ty‐eight thousands seeds of CIANT １８４ were irradiated with gamma‐ray atdosage of ４００ , ４２０ , ４４０ , ４６０ , ５００ , ６００ , ７００ Gy , respectively , in ２００３ . The seedlings were exposed to ２‐３ ℃ in grow thchambers for ３ days as chilling treatment . The surviving plants were maintained and subjected to a second round of selectionafter ３ months . M２ and M３ plants were selected in ２００４ and ２００５ , respectively , like the M１ plants . Five mutants withincreased chilly tolerance and ten dwarf mutants were selected .M４ and M５ plants of mutants were evaluated in greenhouse and fields in ２００６ and ２００７ . The result indicate that in terms ofchilly‐tolerant mutants , M４ plants ( lines ４‐１３ , ４ .２‐１４ , ４ .４‐３ , ６‐２ , and ７‐１) showed lower ion leakage and higher Fv / Fm thanthe wild type control under chilling stress in grow th chamber . In field tests , the mutants had the similar yield to the wild typecontrol in the first year (２００６) , but four lines ( ４‐１‐３‐８ , ４ .４‐３ , ６‐２ , and ７‐１ ) had significant higher yield in the second year(２００７) . Lines ６‐２ and ７‐１ showed early recovery grow th in spring , and had better survival after over‐winter .In terms of the dwarf mutants in greenhouse , the two‐month‐old M５ plants ( lines ４ .２‐２ , ４ .２‐３ , ４ .２‐４ , ４ .２‐６ , ４ .２‐１１ and ７‐２ ) were ３１‐３４％ shorter than the wild type ( the control) , however , the dry weights of shoots and roots of the mutant lineswere similar to those of the wild type , and the dry weight of the shoot of line ４ .２‐３ was even higher . Grow th of the dwarfmutants was promoted by treatment with ５０ mg /L and １００ mg / L gibberellin ( GA３ ) , while CIANT １８４ was not affected ,indicating that GA３ biosynthesis might be blocked in the mutants . All the mutant lines increased drought resistance , and thelines ４ .２‐４ , ４ .２‐６ , and ４ .２‐１１ increased chilly resistance . In field study , the tested mutant lines ( ４ .２‐３ , ４ .２‐４ , ４ .２‐６ , ４ .２‐１１ , ７‐２ ) were approximately ３０％ shorter than their parent cultivar , CIANT １８４ in ２００６ and ２００７ , respectively . Thechlorophyll contents in leaves were also higher in the mutant lines than CIANT １８４ . In spring of ２００７ , M４ plants of dwarfmutants showed early recovery grow th and significantly increased yields .

Breeding for Cold‐Tolerant Lines of \u3ci\u3eStylosanthes guianensis\u3c/i\u3e in Southern China

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3290&amp;context=igc

Breeding for Cold‐Tolerant Lines of \u3ci\u3eStylosanthes guianensis\u3c/i\u3e in Southern China

Abstract

Similar works

Full text

Available Versions

University of Kentucky