Pardeep, M. Andrew

Prabha, T. R.

Ramachandran, M. Mardhu

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings 21st International Grassland Congress / 8th International Rangeland Congress Seasonal Occurrence of Bovine Mastitis Causing Organisms from Grazing Land of Western Ghats, India M. Mardhu Ramachandran The American College, India M. Andrew Pardeep The American College, India T. R. Prabha The American College, India Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/9-3/9 The 21st International Grassland Congress / 8th International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 ]126　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems——— Livestock Production SystemsSeasonal occurrence of bovine mastitis causing organisms from grazing land of western Ghats ,IndiaM .Mardhu Ramachandran 倡 ,M .A ndrew Pardeep ,T .R .PrabhaDepartment o f Immunology & Microbiology ,The American College ,Madurai‐625002 ,India . 倡 E‐mail : mmarudhu＠ si f y .comIntroduction Mastitis is an inflammatory condition of the udder ,charecterized by physical ,chemical and microbiological changesin the milk ; pathological changes in the milk include changes of colour ,consistency and presence of abnormally large numbers ofleucocytes . A variety of bacterial ,viral and fungal species are associated with the inflammatory condition .Some importantbacterial species associated with mastitis are Strep tococcus agalatiae ,Staphy lococcus aureus .The high seasonal prevalence ofBovine Mastitis Causing Organism from Grazing Land of Western Ghats during summer was mainly due to the rough grazing .The present study focuses on the occurrence of Strep tococcus agalatiae and antagonist Pseudomonas spp in the grazing zoneMaterials and methods Four soil samples were collected each month in sterile plastic bags from various places of the grazing landand rain forest zone of the Western Ghats ( Lat .１０°１２′N ,Long ７７°３０′E) in different season( January — December) Tamil Nadu ,India . The soil samples were saturated with sterile distilled water and serially diluted ,and plated onto selective and differentialmedia . These plates were incubated at ３７°c for ２４ hrs ,and isolates were identified using standard biochemical tests ( Bergeysmanual of systematic bacteriology) .Antagonistic properties among the isolates were identified by crowded plate technique .Result Eight different isolates were identified in the grazing zone and rain forest zone , E . coli , Strep tococcus . lactisStaphy lococcus .aures Pseudomonas aeruginosa ,Proteus .vulgaris ,Bacillus sp p ,Entrobacter sp p ,Citrobacter f reundii . Themajor causative agent ,Strep tococcus agalatiae , for mastitis isolated from the grazing zone during the months of April‐Maywhich were found to be completely absent in the rain forest zone . We also found the occurrence of antagonistic Pseudomonasspp against Strep tococcus agalatiae in the grazing zone .The above mentioned isolates failed in antagonistic effect .Figure 1 Seasonal distribution o f Streptococcus agalatiae and Pseudomonas aeroginosa .Discussion Strep tococcus agalatiae is an obligate parasite of the bovine mammary gland which can also survive to a limitedextent in the environment . These invade and multiply in the lactiferous duct . The seasonal incidences of the disease suggestthat an increased disease pattern in summer . The transmission ,colonization and infection are more severe during summer ( dueto rough grazing ) ,and this leads to damage of the teats . This plays a significant role in the occurrence of Strep tococcusagalatiae ,in that the seasonal condition favors the active transmission of the organisms to the host ,resulting in mastitis .Spraying pseudomonas culture in a grazing zone can be a remedial measure to control the occurrence of Strep tococcus agalatiae .ReferencesBradley ,A .J . and Green . ( ２０００ ) .The importance of chronic infection and adaptation of Escherichia coli to the mammaryenvironment‐preliminary UK data . Cattle Practice ,８ ,２３３‐２３７ .Green ,L . (２０００) .Latest situation on low SCC and subsequent intramammary infection . Cattle Practice ,８ ,２３９‐２４１ .Lewis ,s ; Cockcrof t ,P .D ; Bramley ,R .A . and Jackson ,P .G .G . (２０００) .The likelihood of sub clinical mastitis in quarters withdifferent types of teat lesion in the dairy cow . Cattle Practice ,８ ,２９３‐２９９ .

Seasonal Occurrence of Bovine Mastitis Causing Organisms from Grazing Land of Western Ghats, India

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Seasonal Occurrence of Bovine Mastitis Causing Organisms from Grazing Land of Western Ghats, India

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