Gu, Hongru

She, Jianming

Zhang, Jianli

Zhong, Xiaoxian

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings 21st International Grassland Congress / 8th International Rangeland Congress Technique of Plant Regeneration from Immature Inflorescence of Pennisetum Purpureum in Vitro Xiaoxian Zhong Jiangsu Academy of Agricultural Sciences, China Jianming She Jiangsu Academy of Agricultural Sciences, China Hongru Gu Jiangsu Academy of Agricultural Sciences, China Jianli Zhang Jiangsu Academy of Agricultural Sciences, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/1-6/17 The 21st International Grassland Congress / 8th International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 ]266　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅰ 　Grasslands/Rangelands Resources and Ecology ——— Ecology of Grasslands/RangelandsTechnique of plant regeneration from immature inflorescence of Pennisetum Purpureum in VitroZHONG X iaox ian ,S H E Jianming ,GU Hongru , ZHA NG JianliInstitute o f animal Science , Jiangsu A cademy o f A gricultural Sciences , Nanj ing 210014 .E‐mail :x iaox ian＠ j aas .ac .cnKey words :Pennisetum Purpureum ,immature inflorescences ,in v itro ,callus ,plant generationAbstract Immature inflorescences of Pennisetum Purpureum and MS improved and were used for explant and basic culturemedium . Effects of development period of immature inflorescences and hormone compositions of different kinds and ration oncallus induction and plant regeneration were studied . Results indicated that ２ ～ ５ cm immature inflorescences was optimum .The frequency of callus of compact , small pellet induction reached separately ７９ .０ ％ and ７２ .６％ in the callus induction mediumsupplemented with ４ .０mg/ L ２ ,４‐D ＋ ０ .０５ mg/ L KT and ４ .０mg/ L ２ ,４‐D ＋ ０ .１ mg /L KT ( Figure １ ) . During subculture ,Callus of small pellet were maintained ４０ .９％ and ７４ .０％ in the callus subculture medium added ３ .０mg/ L ２ ,４‐D ＋ ０ .２ mg /L６‐BA . The rate of green plant regeneration of small pellet callus from subcultures reached respectively ３６ .４％ and ３８ .５％ inthe differentiation medium supplemented with ２ .０mg /L CPPU ＋ ０ .０１mg /L NAA or ０ .５mg/ L KT ＋ ０ .５mg / L IAA . Greenplant of regeneration with three leaves was transferred to root vigor medium added ０ .５ mg/ L NAA in １ /２ MS basic culturemedium ( Figure ２) . The survivor rate of green plant cultured in soil reached above ９５％ ( Figure ３) . It was a simple effectivemethod to overcome the obstruction of plant generation by selecting the callus of dry , compact , small pellet in early generation .Figure 2 The intact p lantletsf rom immature in f lorescenceso f Pennisetum Purpureum .　 Figure 1 Calli o f dry , compact , smallpellet .　 Figure 3 Regenerated p lant transp lanted insoil .

Technique of Plant Regeneration from Immature Inflorescence of \u3cem\u3ePennisetum Purpureum in Vitro\u3c/em\u3e

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=2445&amp;context=igc

Technique of Plant Regeneration from Immature Inflorescence of \u3cem\u3ePennisetum Purpureum in Vitro\u3c/em\u3e

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