Li, Xingyou

Mi, Fugui

W., Yong

Xu, Chunbo

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings 21st International Grassland Congress / 8th International Rangeland Congress The Effect Factors on Regeneration System of Tissue Culture Using Mature Embryo of Wheatgrass Chunbo Xu Chinese Academy of Agriculture Sciences, China Fugui Mi Inner Mongolia Agricultural University, China Yong W. Inner Mongolia Agricultural University, China Xingyou L. Chinese Academy of Agriculture Sciences, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/1-6/2 The 21st International Grassland Congress / 8th International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅰ 　 瞯 ]251　 瞯Grasslands/Rangelands Resources and Ecology ——— Ecology of Grasslands/RangelandsThe effect factors on regeneration system of tissue culture using mature embryo of wheatgrassX .Chunbo1 , M .Fugui2 , W .Yong2 , L .X ingyou11 G rassland Research Institute o f Chinese A cademy o f A griculture Sciences , H uhhot , Inner Mongolia 010019 P畅R .China ,E‐mail :xcb972002＠ 163 .com 2 Inner Mongolia A gricultural UniversityKey words :wheatgrass , genotype , tissue culture , mature embryo , ABAIntroduction It is the development trend that breeds new variety with good quality , high yield and multiresistance by transgenictechnology in modern wheatgrass breeding . It is precondition of transgene that found a highly‐effect and stabilized regenerationsystem for tissue culture . Through the experiment on tissue culture using mature embryo of differentials genotype wheatgrassto select excellent genotype of wheatgrass that was suitable for transform exogenous gene . The effect factors of callus inductionand regenerated frequency were studied in the regeneration system .Materials and methods The seeds of A . cristatum cv . Fairway( Fairway) , A . desertorum cv . Nordan( Nordan) , A . mongolicumKeng and A .cristatum × A .desertorum cv . Mengnong ( Mengnong ) were choosen to sterilize and embryos of them wereembedded culture medium to induced the callus . Cultures were incubated in the dark at ２６ ℃ for １４ days followed by asecondary culture for ２０ days that repeated twice or thrice . Then the good callus was picked out and cultivate in differentialmedium in the light for １６ hours . The cultures were switched to enrooting medium until the seedlings could survive perfectly .Results Effect of different genotype wheatgrass on inducing callus from mature embryo :All materials for experiment could beinduced to form callus in same medium . The time on appearing and forming of callus were little different . But there were muchdifferent in inducing rate and morphology of callus among ４ materials . The percentage of callus induction for A . mongolicumKeng was higher ２０％ than others . The percentage of callus induction for Nordan was ６７ .５％ , lowest among ４ materials . Inaddition , the morphology of callus of A . mongolicum Keng . possessed compact and dense structure were best than otherspossessed sof t and clearing structure ( Table １ ) .Table 1 E f f ect o f di f f erent genoty pe wheatgrass on inducing callus f rom mature embryo .Varieties Inoculate No . Time ofappearingcallus( d)Time offormingcallus( d)CallusNo .( piece)Percentageof callusinduction ( ％ )morphologyof callusFairway ４００ �４ 殮１０  ３０７ 噰７６ 　.８ white ,soft ,waterNordan ４００ �４ 殮１０  ２７０ 噰６７ 　.５ white ,soft ,waterA . mongolicum Keng ４００ �３ 殮８ 沣３８０ 噰９５ 　White ,compactMengnong ４００ �３ 殮９ 沣２９６ 噰７４ 　white ,soft ,waterDifferentiation and enrooting ability of callus from mature embryo for different genotype wheatgrass : There are remarkablydifferent in percentage of differentiation callus from mature embryo among ４ materials . The seedlings were appeared after theinducted callus of A . mongolicum Keng switched to differentiation for １５ days , but to Mengnong and Fairway , its need ２０days . The seedlings were appeared af ter the inducted callus of Nordan switched to differentiation for ３０ days . And theDifferentiation percentage of callus of A . mongolicum Keng , Mengnong , Fairway and Nordan were ７２％ , ５８％ ,５２％ and ４１％respectively . When the seedlings is ２‐３cm , its were inoculated in enrooting medium . The roots of all materials could be growedand they were robust .Effect of ABA on morphology and differentiation ability of callus from mature embryo of wheatgrass : The secondary culturewas done in medium appended ABA . The result showed that ABA can improve the morphology of callus and enhance thedifferentiation ability on ４ materials . There were different effects to morphology of different callus by different ABAconcentration . When ABA concentration was ０ .１mg / L , it was little effect to callus . But when the ABA concentration was ０ .５mg /L , the quality of all callus was decline . The optimal ABA concentration was ０ .３ mg / L , it could improve the quality ofcallus evidently .Conclusions The callus from mature embryo of A . mongolicum Keng was higher than others in the percentage of callusinduction and differentiation callus . A . mongolicum Keng was a perfect host to transgene . The ABA ( ０ .３ mg/ L ) canremarkably improve the quality of callus .ReferencesL .Yi , G . Junshan , L .Yan . Effect of Different Media on Regeneration of Immature Embryo of Wheat ( T ritium aestivum ) .Journal o f A nhui A gricultural University [ J] .２００３ , ３(１ ) :６‐９ .H .Xuelin . Morphogenetic study on tissue culture of higher plant in vitro [ M ] .Beijing . Science Press .１９９５ .

The Effect Factors on Regeneration System of Tissue Culture Using Mature Embryo of Wheatgrass

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The Effect Factors on Regeneration System of Tissue Culture Using Mature Embryo of Wheatgrass

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