Shi, Decheng

Yan, Hong

Yin, Shangjun

Zhao, Wei

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings 21st International Grassland Congress / 8th International Rangeland Congress Citric Acid Accumulation of Puccinellia tenuiflorais a Specific Adaptive Response to Alkaline Stress Hong Yan Northeast Normal University, China Shangjun Yin Zhejiang Wanli University, China Wei Zhao Air Force Avigation University, China Decheng Shi Northeast Normal University, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/1-4/50 The 21st International Grassland Congress / 8th International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅰ 　 瞯 ]199　 瞯Grasslands/Rangelands Resources and Ecology ——— Ecology of Grasslands/RangelandsCitric acid accumulation of Puccinellia tenui f lora is a specific adaptive response to alkalinestressHong Y an １ ,Shang‐j un Y in２ ,Wei Zhao３ ,De‐cheng Shi １倡１ College o f L i f e Sciences , Northeast Normal University , Changchun , PR China . ２ Department o f L i f e Sciences , Zhe j iangW anli University , N ingbo , PR China . ３ The Department o f A v igation L i f esav ing , A ir Force A v igation University ,Changchun , PR China . E‐mail address :shidc２７４ ＠ nenu .edu .cnKey words : Puccinellia tenui f lora , saline stress , alkaline stress , organic acid , citric acid , prolineIntroduction Puccinellia tenui f lora is typical forages found in saline‐alkaline habitats of Songnen Grassland . Owing to thespecial osmregulation , the species could survive in high pH conditions . In order to understand the physiologic characteristic ofP . tenui f lora alkali tolerance , citric acid accumulation under the alkaline stress was studied .Materials and methods These treatments were divided randomly into １５ groups (３ replicates per group) . One group was used asthe control ( CK ) . Five groups were treated with Na２ CO３ solutions . Six groups were treated with NaCl solutions withconcentrations . The remaining ３ groups were treated with １００ mmol l‐１ Na２ CO３ solutions , which were neutralized pH７ , pH８and pH９ using ８５％ H３ PO４ . Proline condentration was determined according to Bates et al . (１９７３) . The concentration of totalorganic acid was determined according to Jing and Ding ( １９８１ ) , and citric acid was determined using the pentabromoacetonemethod adapted from Shi and Yin (１９９３) .Results Citric acid concentrations of P . tenui f lora increased with increasing stress strength by Na２CO３ ( p ＜ ０ .０５ , R ＝ ０ .９６７) .Under NaCl stress , citric acid concentration decreased slightly ( p ＞ ０ .０５) w ith increasing stress strength ( Figure １) . Changesin the concentration of citric acid and total organic acid with increasing stress strength were almost parallel in the alkalinestressed seedlings of P . tenui f lora ( data not shown) . The proline concentration of P . tenui f lora also increased with theincreases of NaCl and Na２CO３ . In neutralization treatments , citric acid concentrations of P . tenui f lora decreased withdecreasing pH value ( p ＜ ０ .０５ , R ＝ ０ .９６３) . Proline concentration of changed irregularly with pH value decreasing .Figure 1 Comparison o f citric acid and p rolineaccumulation between salt stress and alkaline stress in P .tenuiflora .Figure 2 E f f ects o f neutraliz ation on the concentrationso f p roline and citric acid in P .tenuiflora .Conclusions Under alkaline stress , the organic acid metabolism of P . tenui f lora was changed , mainly due to the accumulationof citric acid . The citric acid accumulation was a specific physiological response to alkaline stress . The proline accumulation wasphysiological response to osmotic stress and the primary physiological significance of proline accumulation was osmoticadjustment .ReferenceShi , D .C . , and Yin , L .J . (１９９３) . Difference between salt ( NaCl) and alkaline ( Na２CO３ ) stresses on Puccinellia tenui f lora( Griseb .) Scribn . et Merr . plants . A cta Botanica Sinica , ３５ : １４４‐１４９ . ( in Chinese) .0.3 0.18 O.Q7 --0- salt-citrc acid stress □ proline ~ alkaline-citrc acid 0.16 0.25 0.06 ~salt-proline 0.14 i [ ~ alkaline-proline ~ 0.05 0.2 '° 0.12 ] '° "" 0 0.1 '° .§. 0.04 E ] .§. 0.15 -0 -~ -0 0.08 .§. u ·~ .S ·.s O.oJ ·~ 0.1 0.06 1 ·a :; " 0.02 0.04 C 0.05 l 0.02 0.0 1 0 100 150 200 250 300 400 Na+ concnetration (mM) pH6.8 pH 7 pH 8 pH 9 pH 1 I. OS 

Citric Acid Accumulation of \u3cem\u3ePuccinellia tenuiflorais\u3c/em\u3e a Specific Adaptive Response to Alkaline Stress

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Citric Acid Accumulation of \u3cem\u3ePuccinellia tenuiflorais\u3c/em\u3e a Specific Adaptive Response to Alkaline Stress

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