Gu, Hongru

She, Jianming

Zhong, Xiaoxian

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings 21st International Grassland Congress / 8th International Rangeland Congress Effect of NaCl Concentration on Embryogenic Callus Growth and Plant Regeneration of Pennisetum Purpureum Schumach (Napier Grass) in Vitro Xiaoxian Zhong Jiangsu Academy of Agricultural Sciences, China Jianming She Jiangsu Academy of Agricultural Sciences, China Hongru Gu Jiangsu Academy of Agricultural Sciences, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/1-4/11 The 21st International Grassland Congress / 8th International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 ]160　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅰ 　Grasslands/Rangelands Resources and Ecology ——— Ecology of Grasslands/RangelandsEffect of NaCl concentration on embryogenic callus growth and plant regeneration of PennisetumPurpureum Schumach (Napier grass) in vitroZhong X iaox ian , She j ianming , Gu HongruInstitute o f animal Science , Jiangsu A cademy o f A gricultural Sciences , Nanj ing 210014 , China . E‐mail : x iaox ian＠ j aas .ac .cnKey words : Pennisetum Purpureum , napier grass , salt‐tolerant , sodium chloride , tissue cultureIntroduction Pennisetum americanum ( Napier or elephant grass) and P .americanum × P .purpureum ( Hybrid Pennisetum) areimportant forage and energy crops . In order to guarantee food safety , breeding salt‐tolerant napier grass and hybrid Pennisetumare of great significance for utilizing plentiful arid and saline land to satisfy need of livestock and production of biomass energyin China . Only one paper reported selection of NaCl tolerant cells from leaf‐derived embryogenic cultures of napier grass tillnow ( Stephen and INDRA , １９８４ ) . This report describes effect of NaCl concentration in medium on from immatureinflorescences embryogenic callus grow th and plant regeneration of Pennisetum Purpureum N５１ ,which is R line of registeredvariety �Hybrid pennisetum"( Tif t ２３A CMS pearl millet × N５１ Napier grass) .Materials and methods Embryognic callus was initiated from young inflorescences (１‐３ cm in length) of Pennisetum purpureumSchum ( N５１) . Using sterilization and dissection techniques previously described ( Zhong et al . ,２００７ ) . A white and compactembryogenic callus pieces obtained from immature inflorescences were transferred to subculture medium supplemented with １ .０ , １ .２ , １ .４ , １ .６ , １ .８ and ２ .０ g / L NaCl . Af ter ４５ days healthy embryogenic callus was grown on differentiation medium withsame NaCl concentration . All cultures were incubated at ２６‐２８ ℃ in a grow th chamber under １６h of diffused light .Table 1 E f f ect o f NaCl concentration in medium on rate o f compact callus and rate o f regeneration p lant .NaCl ( g / L ) NO . of total callus SubcultureRate of compact callus ( ％ ) Differentiation culturePercentage of plantlet ( ％ )０  ６７６ 滗５５ 亮.３ ８ è.４１０  ２２６ 滗４９ 亮.３ ４ è.０１２  ３３７ 滗３６ 亮.２ １ è.８１４  ３６５ 滗３２ 亮.８ １ è.６１６  １５７ 滗３２ 亮.２ ０ è.６１８  ３０４ 滗３４ 亮.１ ０ è.３２０  １３５ 滗１５ 亮.４ ０ è.０Results There was a decrease in ration of compact callus and percentage of plantlet with salt concentration increase ( Table １ ) .The pellet callus became less well organized and more watery appearance and light brown color above１ .２ g / L NaCl . Rate ofcompact callus and percentage of plantlet was １５ .４％ and ０ .０％ at ２ .０ g / L NaCl . All compact callus was necrotic af ter ２ weeksabove ２ .０ g / L NaCl . This result showed critical NaCl concentration limit was ２０ g / L during subculture of small pellet callusand lethal NaCl concentration was respectively ２０ g / L in differential culture .Conclusions NaCl concentrations in medium have obvious effect on embryogenic callus grow th and plant regeneration of Napiergrass in v itro in this study . Callus cultures tolerant to normally inhibitory concentrations of sodium chloride were ２０ g / L insubculture and １８ g / L in differential culture . Salt tolerance of plant regenerated from callus selected at high salt levels needfurther assessed .ReferenceSTEPHEN FC and INDRA KV .( １９８４ ) . Selection and characterization of NaCl tolerant cells from embryogenic cultures ofPennisetum Purpureum Schum . ( Napier or elephant grass) . Plant Science letters , ３７ ,１５７‐１６４ .

Effect of NaCl Concentration on Embryogenic Callus Growth and Plant Regeneration of \u3cem\u3ePennisetum Purpureum\u3c/em\u3e Schumach (Napier Grass) \u3cem\u3ein Vitro\u3c/em\u3e

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=2339&amp;context=igc

Effect of NaCl Concentration on Embryogenic Callus Growth and Plant Regeneration of \u3cem\u3ePennisetum Purpureum\u3c/em\u3e Schumach (Napier Grass) \u3cem\u3ein Vitro\u3c/em\u3e

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