Neyraiella distinctus n. sp. was found parasitizing nymphs of the cricket Gryllodes laplatae Sauss in City Bell, Argentina. This species was characterized by having the excretory pore in the posterior end of the basal bulb, vulva protruding with one lip well developed in the 1/3 end of the body, anus of the female with wings, male with a single spicule without any sculpture, gubernaculum and bursa are absent, six pairs of genital papillae arranged in two preanal pairs, one adanal pair and three postanal pairs, and the tail appendage in both sexes was short and conic.Facultad de Ciencias Naturales y Muse

Camino, Nora Beatriz

Villalobos, Leonor Cristina de

Memórias do Instituto Oswaldo Cruz

English

El Servicio de Difusión de la Creación Intelectual

325Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 97(3): 325-327, April 2002
Neyraiella distinctus n. sp. (Oxyurida, Blattophilidae) Parasite of
Nymphs of Gryllodes laplatae Sauss (Orthoptera, Gryllidae)
in Argentina
Nora B Camino/+, Cristina de Villalobos*
 Centro de Estudios Parasitológicos y de Vectores, Calle 2, 584, 1900 La Plata, Argentina  *Facultad de Ciencias Naturales y
Museo, La Plata, Argentina
Neyraiella distinctus  n. sp. was found parasitizing nymphs of the cricket Gryllodes laplatae Sauss in City Bell,
Argentina. This species was characterized by having the excretory pore in the posterior end of the basal bulb, vulva
protruding with one lip well developed in the 1/3 end of the body, anus of the female with wings, male with a single
spicule without any sculpture, gubernaculum and bursa are absent, six pairs of genital papillae arranged in two
preanal pairs, one adanal pair and three postanal pairs, and the tail appendage in both sexes was short and conic.
Key words: Neyraiella distinctus n. sp. - Oxyurida - parasite - cricket - taxonomy - Argentina
Sanchez (1947) described in Spain a parasite of
Gryllotalpa europeus  L. (Orthoptera: Gryllotalpidae), and
placed it within the oxyurids. He created the genus
Neyraiella and the new Family Neyraiellidae, with the
type species Neyraiella neyrae.
Skrjabin et al. (1951,1954), Theodorides (1953), Basir
(1956), Rao (1958), Kloss (1959), and Leibersperger  (1960)
accomplished a taxonomic revision of the oxyurids. These
authors think that Serrano Sanchez (1947) makes a mis-
take when describing the males of N. neyrae with two
spicules instead of one, and therefore considering N.
neyrae as a synonym of Gryllophila skrjabini (Sergiev)
Basir, 1956 and placing this species within the family
Thelastomatidae.
Poinar (1975), modified the treatise on invertebrates
of Skrjabin et al. (1966), and gave a new key for the Order
Oxyurida. He separated the superfamilies Thelastoma-
toidea and Rhigonematoidae by the presence in the fe-
male of eight and four cephalic papillae, respectively. The
males in the Thelastomatidae family present only one spi-
cule, while in the Rhigonematidae they present either one
or two rudimentary spicules. Based on these features, he
established Neyraiella as a valid genus, placing it within
the superfamily Rhigonematoidea, family Blattophilidae.
In this paper we report another new species of the
genus Neyraiella, N. distinctus sp. n. parasite of Gryllodes
laplatae Sauss (Orthoptera: Gryllidae) found in City Bell,
Argentina.
MATERIALS AND METHODS
Nymphs of G. laplatae collected manually were kept
in plastic containers. In the laboratory the techniques of
Poinar (1975) were followed, consisting on cooling the
nymphs to 5ºC approximately 3 min, and then dissecting
+Corresponding author. Fax: +54-221-423.2327. E-mail:
nemainst@cepave.com.ar
Received 28 June 2001
Accepted 19 December 2001
them under electronic microscope in Petri dishes with dis-
tilled water. Nematodes (males and females) were extracted
from the intestine stomodeus and were killed in distilled
water in a double boiler during 2 min. Later they were kept
48 h in a solution of 50% TAF and 50% distilled water,
and then fixed in pure TAF. Measurements and drawings
were made with a Zeiss microscope with camera lucida.
Measurements given in the description belong to the male
holotype and female allotype, the range of measurements
of the paratypes are given between parentheses.
RESULTS
Order Oxyurida
Superfamily Rhigonematoidea
Family Blattophilidae
Genus Neyraiella
Neyraiella distinctus sp. n.
(Fig. 1 A-F)
Description: small and white nematodes. Annulated cu-
ticle. Mouth surrounded by four cephalic papillae. Bucal
cavity with small denticles (Fig. 1C). Small and pyriform
amphids. Esophagus long with indistinct pseudobulb (Fig.
1D,E). Basal bulbs with valves. Excretory pore opening
behind the basal bulb. Vulva protruding with vulval lips
developed and placed in the posterior third of the body
(Fig. 1A). Vagina cylindrical and long directed to the pos-
terior end. Female prodelphic. Uterus with strangulations
forming true capsules, each one containing a single egg.
Female anus with wings. Eggs oval, with smooth shell,
without byssum and individual (Fig. 1A). Male with a
simple spicule not sculptured, without gubernaculum or
bursa  (Fig. 1F). Genital pore opens in a protuberance. Six
pairs of genital papillae as follows: two preanal pairs, one
pair adanal and three pairs postanal, the last of which is
placed at the base of the tail appendage (Fig. 1B). Tail in
both sexes short and conic.
Male (n = 15) - Total body length: 2.050 µm (1.980-2.120),
cephalic diameter at level of papillae: 18.8 µm (16.45-21.15);
maximum body diameter: 108.1 µm (91.65-124.55); distance
from anterior end to base of basal bulb: 245 µm (220-270);
spicles length: 36.42 µm (35.25-37.60); tail length: 17.62
µm (14.10-21.15).
326 New Oxyurida Parasite of Cricket  NB Camino, C de Villalobos
Female (n = 20) - Total body length: 4.420 µm (4.280-4.560);
cephalic diameter at level of papillae: 24.67 µm (23.5-25.85);
body diameter at level of vulva: 272.5 µm (265-280); maxi-
mum body diameter: 440 µm (400-480);distance from ante-
rior end to base of basal bulb: 515 µm (460-570); distance
from vagina to anus: 675 µm (610-740); V: 82.69% (82.67-
82.71); tail length: 29.37 µm (25.85-32.9); length and width
of eggs: 85.77 µm (70.50-101.05) x 32.9 µm (30.55-35.25).
Type locality: City Bell, La Plata, Buenos Aires Province,
Argentina.
Type Host: nymphs of Gryllodes laplatae Sauss (Ortho-
ptera: Gryllidae).
Type material: deposited in the Helmintological collec-
tion, Museo de La Plata, La Plata, Argentina, no. 4848.
DISCUSSION
We agree with Poinar (1975) considering valid the ge-
nus Neyraiella. N. distinctus sp. n. shares with the single
species of the genus hitherto described, N. neyrae
Sanchez, 1947, the following characters: transverse cu-
Neyraiella distinctus n. sp. A: posterior end of the female, vagina, eggs and anus; B: posterior end of the male, ventral view; C: anterior end
of the female; D: stoma of the female; E: anterior end of the male; F: posterior end of the male, lateral view. Bars = 50 µm
327Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 97(3), April 2002
ticle striae, mouth surrounded by four papillae, amphids
small and pyriform, uterine rami with stranglings forming
capsules for each egg, eggs with smooth shell, in the male,
protruding genital cone, and the presence in both sexes
of a short and conic tail.
N. neyrae differs from our new species in the follow-
ing characters: bucal cavity with two ribs forming a fork,
and ending each one in a denticle, the excretory pore opens
much behind the base of the esophagus, the vulva is not
protruding, the vagina isshort, the eggs are large (150 µm
x 70 µm), the spicule is longer (63 µm) and it lacks the
adanal pair of papillae.
Because of the mentioned characters we regard N.
distinctus sp. n. as a new species.
REFERENCES
Basir MA 1956. Oxyuroid parasites of Arthropoda. Zoologica
38: 1-79.
Kloss GR 1959. Nematoides parásitos de Gryllotalpoidea (Or-
thoptera) do Brasil. Mem Inst Oswaldo Cruz 57: 137-170.
Leibersperger E 1960. Die Oxyuroidea der euroäischen
Arthropoden. Parasitol Schriftenr Jena 11: 1-150.
Poinar Jr GO 1975. Entomogenous Nematodes. A Manual and
Host List of Insects Nematode Associations, EJ Brill, Leiden,
317 pp.
Rao PN 1958. Studies on the nematode parasites of insects and
other arthropods. Arquiv Mus Nac Rio de Janeiro 46: 33-
84.
Sanchez AS 1947. Nematodes parasitas intestinales de los
artropodos en España. Rev Iber Parasitol 7: 279-332.
Skrjabin KJ, Schikhobalova NP, Lagodovskaya EA 1966.
[Manual of Nematology] Nauka, SSSR, 538 pp. (in russian).
Skrjabin KJ, Schikhobalova NP, Mosgovi AA, 1951. [Descrip-
tive catalogue of nematodes parasites]. Acad Cs Urss Lab
Helmintol Moscu 2: 631 (in russian).
Skrjabin KJ, Schikhobalova NP, Sobolev AH, Paramonov AA,
Sularikov VE 1954. [Descriptive catalogue of nematodes
parasites]. Acad Cs Urss Lab Helmintol Moscu 4: 927 (in
russian).
Theodorides J 1953. Sur un nématode parasite de la courtilière
Gryllotalpa gryllotalpa (L.) (Orthoptera:Gryllotalpidae):
Gryllophila skrjabini (Sergiev) Basir var. ovipolita nov.
(Oxyuroidea, Thelastomatidae). Ann Parasit Hum Comp
28: 300-304.


Neyraiella distinctus n. sp. (Oxyurida, Blattophilidae) parasite of nymphs of Gryllodes laplatae sauss (Orthoptera, Gryllidae) in Argentina

http://sedici.unlp.edu.ar/bitstream/handle/10915/29653/Documento_completo.pdf?sequence=1

Neyraiella distinctus n. sp. (Oxyurida, Blattophilidae) parasite of nymphs of Gryllodes laplatae sauss (Orthoptera, Gryllidae) in Argentina

Abstract

Similar works

Full text

Available Versions

El Servicio de Difusión de la Creación Intelectual