학위논문 (석사)-- 서울대학교 대학원 : 약학대학 약학과, 2018. 8. 신종헌.Abstract
Isolation and Structure Determination of Bioactive Constituents from Poncirus trifoliata Raf.
Won-Hee Lee
Natural Products Science Major
College of Pharmacy
Master Course in the Graduate School
Seoul National University
Sortase A (SrtA), a gram-positive transpeptidase, is an important target in reducing bacterial infection. The extract from the fruits of trifoliate orange, Poncirus trifoliata Rafinesque, showed significant Srt A inhibition.
Poncirus trifoliata Raf. is a member of the family Rutaceae and it has been widely used in Korean traditional medicine for the treatment of diverse pathogenic symptoms.
Bioactivity-guided chromatographic separation afforded the isolation of in total twelve compounds, eight coumarins and four flavanone glycosides.
The structures of four novel coumarins were determined by a combination of spectroscopic analyses such as NMR and LC-MS.
The absolute configurations of the new coumarins and known coumarins, previously unassigned, were determined by an application of the 2-methoxy-2-(trifluoromethyl)phenylacetic acid (MTPA) method.
Key words: herbal medicine, Poncirus trifoliata Rafinesque, coumarins, flavanone glycosides, sortase A inhibition
Student number: 2016-29626Introduction . 1
Experimental Section 3
1. General Experimental Procedures 3
2. Plant Material . 3
3. Extraction and Isolation 4
4. Preparation of the (S)- and (R)-α-Methoxy-αtrifluoromethylphenylacetic chloride (MTPA-Cl) Esterification of Compound 1-6 6
Results . 12
1. Compound 1 . 12
2. Compound 2 . 12
3. Compound 3 . 13
4. Compound 4 . 13
5. Compound 5 . 14
6. Compound 6 . 14
7. Compound 7 . 15
8. Compound 8 . 15
9. Compound 9 . 16
10. Compound 10 . 16
11. Compound 11 16
12. Compound 12 . 17
Discussion 40
References . 41
Abstract in Korean . 75Maste
