Background:
In practice, either the herbs themselves or the crude extracts are used for traditional remedies but not the isolated molecules. Therefore, the importance of toxicity and clot lysis study of the crude extract is beyond description.
Objective:
The aim of our study was to find out the preliminary cytotoxic and thrombolytic effect of the seven selected medicinal plant leaves extract.
Methods:
Cold maceration was performed using ethanol for extraction purposes. The cytotoxicity study was done by using brine shrimp
nauplii (Artemia salina) and MTT assays by using MCF-7 cell
line.
Result:
In the cytotoxic activity, out of the seven leaves extract three of them exhibited stronger brine shrimp lethality with 4 TH PHARMACEUTICAL RESEARCH CONFERENCE 2018
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LC50 122.548 (Uncaria acida), 170.861 (Leea indica) and
175.469 (Piper porphyrophyllum) μg /mL respectively, and on
MCF-7 cell line, they also exhibited moderate cytotoxic activity with different concentration of the extract of the same plant leaves such as, Uncaria acida (72.31, 56.22, 38.12 and 9.24%), Leea indica (67.31, 58.22, 43.12 and 15.24%), Piper porphyrophyllum (65.88, 48.12, 40.12 and 21.34%). In thrombolytic assessments, all the leaves extract showed moderate (considering > 20% moderate; **p < 0.01; *p < 0.05) clot lysis activity, but among the extracts, Uncaria cordata (27.36 ± 0.10%) showed the highest and Stachytarpheta indica the lowest (6.14 ± 0.20%) percent clot lysis as compared with the standard streptokinase (65.15 ± 0.16%).
Conclusion:
This study was conducted to support the folkloric use of seven medicinal plant leaves to improve cancer or myocardial infarction.
Although, it is a preliminary screening but it will be targeted to the scientific community to put more attention and to do an extensive and molecular level study in future
