Abdul Rahman, Mohd Norhisham Azmi

Heng, M. S. T.

I. C., Chetter

J., Greenman

M., Fagan

Peter T., McCollum

English

The International Islamic University Malaysia Repository

ESSR Abstracts 1A001–A193Sepsis, Infection, Immunity 2 A001The dose related effect of systemic antibiotics in prevention ofpostoperative adhesion formation in experimental animalsAbbas Mohamed1, Nafeh Ayman2, Elsebae Magdy M A3 andFarouk Youssef41Department of General Surgery-Theodor Bilharz Research Institute, Cairo, Egypt,2Department of General Surgery-Theodor Bilharz Research Institute, 3Departmentof General Surgery -Theodor Bilharz Research Institute, 4Department of GeneralSurgery-Theodor Bilharz Research Institute (magdyelsebae@hotmail.com)Sixty Wistar-Albino rats weighing 200–250 g were assigned to 2 main groups,of 30 rats each. Animals in the first main group (GI, n = 30) were injectedintramuscularly with 1 ml saline, half an hour preoperatively (the main controlgroup). In the second main group (GII, n = 30) animals were injected with50 mg/kg cefepime HC (Maxipime, BM, Egypt) and 7·5 mg/kg metronidazole(Flagyl, Aventis, Egypt) in a volume of 1/2 ml for each (The main antibioticgroup). After a midline laparotomy was performed, abdominal adhesions wereinduced in all animals. After operation, animals in the main control groupwere submitted according to the numbers of postoperative intramuscular salineinjections into 2 subgroups; GIa (n = 15) in which animals were injected every8 hours for 2 doses and GIb (n = 15) where animals were injected every 2 hoursfor 5 days. Similarly, themain antibiotic groupwas subdivided intoGIIa (n = 15)and GIIb (n = 15). On the 14th day, the rats were killed and the adhesion scorewas determined.Results: Short course of antibiotic significantly decreased the extent ofpostoperative peritoneal adhesions (P < 0·05), while the severity of adhesionsdid not show significant changes. The 5 day course of antibiotics reviledsignificant reduction in both the extent (P < 0·001) and the severity (P < 0·01)of postoperative peritoneal adhesions. To conclude, short course antibiotics,which have been the standard regimen for prophylaxis against surgical infection,did not show significant reduction of postoperative intra-abdominal adhesionsin experimental animals. A 5 day course of antibiotics significantly decreasedthe incidence, extent and severity of postoperative intra-abdominal adhesions.However this 5 day course should not be described routinely in abdominalsurgical practice. It may be indicated in prolonged abdominal operations,surgery on the bowels, history of recurrent adhesive intestinal obstruction orrelaparotomy operations.Cardiovascular, Thoracic 1 A002Concentration and activity of matrix metalloproteinases and tissueinhibitors of matalloproteinases in the wall of abdominal aorticaneurysm at different wall stressAbdul Rahman M N A1, Heng M S T2, Fagan M3, Greenman J4,McCollum P T5, Chetter I C61Academic Vascular SurgicalUnit,Hull Royal Infirmary, 2Academic Vascular SurgicalUnit, Hull Royal Infirmary, 3Medical Engineering Department, University of Hull,4Biosciences Department, University of Hull, 5Academic Vascular Surgical Unit,Hull Royal Infirmary, 6Academic Vascular Surgical Unit, Hull Royal Infirmary(hishami.abdulrahman@hey.nhs.uk)Background: AAA formation and development occur due to excessiveextracellular matrix degradation which is closely regulated by matrixmetalloproteinases (MMPs) and their inhibitors (TIMPs). It is suggested thatvariation in wall stresses may be influential in this regulation. We aimed toassess the impact of high and low wall stress on MMPs and TIMPs and tocompare this with controls.Method: We recruited 22 patients undergoing elective AAA repair and 8patients undergoing (CABG) as controls. A 3D CT reconstruction of AAA wasperformed and analysed using FEA for wall stress calculation, where sampleswere taken. Ascending thoracic aorta samples obtained during CABG were usedas controls. All samples were snap frozen and analysed for MMP 2, 8 and 9 andTIMP 1 and 2 using ELISA. Statistical analysis was performed using SPSS v14.Results:All results are in median and IQ rangeHigh wall stress Low wall stressControl MMP 8 active 5·8 (2·6–9·9) 6·3 (3·8–10·8) 3·5 (2·6–5·0) MMP 8 total14·2 (8·9–43·6)* 13·3 (9·2–31·0)* 6·3 (3·2–13·4) MMP 9 active 0·4 (0·29–1·39)*0·6 (0·29–0·86)* 14·8 (7·2–18·1) MMP 9 total 8·1 (3·6–16·1)* 8·3 (5·0–11·6)*25 (19·0–61·0) TIMP 1 296 (164–522)* 176 (82–321) 130 (83–221) TIMP 2 25(11–45)* 18 (10–33)* 174 (134–232) * = p < 0·05 compare to control.Conclusion: Concentration and activity of MMPs and TIMPs in the wall ofAAA may be influence by variation in wall stress.Oncology 2 A003Androgen receptors and reaction for hormonotherapy in lymphnode-positive and lymph node-negative women with breast cancer.Agrawal Anil Kumar1, Zukrowski Piotr2, Grzebieniak Zygmunt3,Jelen Michal4, Slonina Joanna512nd Department of General and Oncological Surgery Wroclaw Medical University,Poland, 22nd Department of General and Oncological Surgery Wroclaw MedicalUniversity, Poland, 32nd Department of General and Oncological SurgeryWroclaw Medical University, Poland, 4Department of Pathology Wroclaw MedicalUniversity, Poland, 5Department of Radiology Wroclaw Medical University, Poland(zubex@gazeta.pl)Introduction: Hormonotherapy in women with locoregional breast cancerimprove prognosis when estrogen receptors or/and progesterone receptors arepositive. The role of the androgen receptors (AR), that are also one of thefamily steroid receptors, is discussed. It is important because of 10% womenhave only androgen receptors on cancer cells. So the question is: should theyget hormonotherapy?Purpose of the study: We’d like to find any correlations between AR,metastases to the regional lymph node and with answer for hormonotherapy(HT) in women with breast cancer.Material and methods: 723 womenwith locoregional breast cancer operatedin 2nd Department of General Surgery Wroclaw Medical University inperiod 2000–2002. All women were divided in 8 groups: −AR+/LN+/HT+,−AR+/LN+/HT−, −AR+/LN−/HT+, −AR+/LN−/HT−, −AR−/LN+/HT+, −AR−/LN+/HT−, −AR−/LN−/HT+, −AR−/LN−/HT−. Weanalyzed the patient documentation in pre- and postoperative period. Theanswer for hormonotherapy we evaluated based on 5-year survival afteroperation and local recurrence.Results: Androgen receptors were positive in 329 women (45,5%). The mostof them didn’t have metastases to regional lymph node – 170 (51,7%). Whearsmany patients AR(+) has breast cancer with N2 or N3. In group with AR(+)and LN(+), that got hormonotherapy 5-years survival was 16% higher and thelocal recurrence was twice less than women with AR(+) and LN(+), that didn’t’get hormonotherapy.Conclusions: Androgen receptors are the most common steroid receptorson breast cancer cells. They were found often in women with stage N2 andN3. The prognosis was better in women with AR(+) and LN(+) who gethormonotherapy. We need more study to recommended hormonotherapy inevery women with AR(+) as routine postoperative treatment in women withbreast cancer.While every effort is made by the Editorial Team and the Publishers to avoid inaccurate or misleading information appearing in BJS, data within theindividual abstracts are the responsibility of the Authors. The BJS society, the Publishers and members of the Editorial Team accept no liability whatsoeverfor the consequences of any such inaccurate or misleading data, opinion or statement.Copyright  2008 British Journal of Surgery Society Ltd British Journal of Surgery 2008; 95(S6): 1–104Published by John Wiley & Sons Ltd

Concentration and activity of matrix metalloproteinases and tissue inhibitors of matalloproteinases in the wall of abdominal aortic aneurysm at different wall stress

http://irep.iium.edu.my/59153/1/59153_British%20Journal%20of%20Surgery.pdf

Concentration and activity of matrix metalloproteinases and tissue inhibitors of matalloproteinases in the wall of abdominal aortic aneurysm at different wall stress

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