'Universitas Indonesia, Directorate of Research and Public Service'
Doi
Abstract
Objective: The purpose of this study was to provide a reference of chronic diabetes complications by investigating the
prolonged hyperglycemia effects on hematological, biochemical and histopathological changes (liver, kidney, spleen,
cardiac muscle, adrenal gland, and endocrine pancreas) in diabetic rats induced by streptozotocin. Methods: Ten adult
female Sprague-Dawley of uniform age were divided into two Groups. Group 1 was made diabetic by single
intraperitoneal injection of streptozotocin (60 mg/kg/bw) whereas Group 2 served as control. After six months, the rats
were anesthetized using pentobarbital. Cardiac puncture was performed to get 3 ml of the blood sample; following 12
hours of an overnight fast. Serum chemistry test and complete blood analysis for lipid profile and blood glucose test;
liver and renal functions were performed. Tissue specimens of liver, kidney, spleen, cardiac muscle, adrenal gland, and
endocrine pancreas were fixed in 10% formal saline and processed for histological study. Results: There were severe
histopathological changes in the affected organs; and the presence of a significant abnormality of lipid profile, liver, and
renal functions. Conclusions: The presence of histopathological changes with abnormal biochemical changes is related
to the chronic absence of insulin production in the destroyed β –cells which reflect the diabetic complications in a
human being
