Kulliyah of Engineering, International Islamic University Malaysia
Abstract
The bioactivity test on the performance of porous microcarriers of pure biphasic calcium phosphate (BCP) and
Mg-doped BCP was conducted in spinner vessel with Vero cell lines for 90 hours. The findings revealed that all
the porous microcarriers were biocompatible as the cells already attached on the microcarrier after 24 hours of
culture. The presence of divalent cations including Mg2+ is encouraged the spreading and adhesivity of cells onto
bioceramic matrices. Magnesium also believed to increase the solubility of BCP in vitro which is useful to establish
cell-material interface and thus improved cell attachment and growth. Thus, higher cell attachment, growth, and
migration of cell deep inside the pores were obtained on microcarriers of 10 mol% Mg-doped BCP compared to
undoped BCP. The substitution of Mg into the BCP present several advantages related to metabolic cell
behaviours as FESEM results showed that Mg substitution can induce a spread-like and irregular morphology
which is quite different from the cell grown onto the pure BCP where the cells just remain on the surface of the
scaffold, or in a localized area within the scaffold and proliferate there. This makes porous Mg-doped BCP
substrate a promising candidate as a bone substitute and microcarrier
