The aim of the present study is to investigate the effect of operating
parameter of a Buchi B290 mini spray dryer towards specific activity of recBromelain.
Using Response Surface Methodology (RSM), face centred central composite design
(FCCCD) was tabulated for optimization. Optimization process was conducted by
varying the inlet air temperature (°C), gas flow height (mm) and pump settings (%) of
the laboratory Buchi B290 Mini Spray Dryer to obtained optimal spray drying
operating processes recovering high recBromelain specific activity as response. Results
showed that the optimized process parameter having 126 °C air inlet temperature, 42
mm gas flow height and 12 % feed pump setting resulted in 0.119 ± 0.003 U/mgprotein
of spray dried recBromelain with R2 of 90.12% thus this model was found to be
significance and reliable
