Ahmad, Hajar Fauzan

Anua, Mohd Hanafi

Hashim, Ridzwan

Kamarudin, Kamarul Rahim

Lukman, Ahmad Lutfi

Nordin, Noor Faizul Hadry

Usup, Gires

English

The International Islamic University Malaysia Repository

KIaIt .I l"\-/  zt/o3 /toa tIAlirltOL*rr'; ...AruDu3f,w?m'oEr.ri@d l,rtlufo thivrnity l{rlrt,t#ffiffi:' Jiffi1 9.h INTERVARSITY BIOCHEMISTRYSEMINARI" S C IIENffi E g,l-?o'vlrE R S CWt [EE"22"d March 2008Faculty of Engineerin g & Science,Universiti Tunku Abdul Rahmanin collaboration withThe Malaysian Society for Biochemistry &Molecular BiologyVenue:Universiti Tunku Abdul RahmanBuilding PB, No. 13 Jalan 131646200 Petaling Jaya, SelangorP-8OSSICLE SHAPES AND MITOCHONDRIAL DNA PROFILESOF Holothzria AND Stichopus SPECIESMohd Hanafi Bin Anual, Kamarul Rahim Kamarudint*, Gires Usup2, RidnranHashim3, Noor Faizul Hadry Nordinr, Hajar F auzanAhmadl & Ahmad LutfiLukmanl1 Department of Biotechnologt, Kulliyyah of Science, International Islamic University Malaysia^ (IIUM), Jalan Istana, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia;"Programme of Maine Science, School of Environmental & Natural Resource Sciences, Faculty ofScience & rechnotogy, Universiti Ke**';;:ryif:;fr|*) 43600 UKM Bangi, setangor Darut3 Department of Biomedical Science, Kulliyyah of Sciince, International Islamic (Jniversity Malaysia(IIUM), Jalan Istanq, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia* C orr esp onding author : p hy s ique4 8 I @y aho o. c o.ukOssicles and mitochondrial DNA (mtDNA) of selected local sea cucumbers(Holothuroidea) were incorporated in this study in order to compare the ossicleshapes between lhe Holothuria and Stichopus species as well as to differentiate themtDNA profiles between the two genera. Samples were collected from two mainlocalities in Peninsular Malaysia namely Tioman Island, Pahang (Eastern region)and Pangkor Island, Perak (Western region). Species from genus Stichopus such as ,S.horrens (golden gamat) and ,S. chloronotus (talifan vqrieti hitam) used in this studyare locally known as gamat and some have been proven scientifically containingmedicinal properties, while Holothuria species such as H. leucospilota (bat puntil)and H. atra (bat hitam) were reported abundant in Malaysia. Species identificationdone by researches in Malaysia was mainly based on the morphologicalcharacteristics and the molecular systematic information of this echinoderm is stilllimited and unclear. Due to such reasons, mtDNA represented by cytochrome b (cyt-b) mtDNA gene and 165 ribosomal mtDNA gene was used in this study to updatethe species validity and relationship, as an alternative to morphological studies usingthe ossicles. Small portion of muscle tissue was digested using conventionalmethods in order to obtain the ossicles. DNA extraction by using kit, PolymeraseChain Reaction (PCR) and phylogenetic analyses were considered as the mainmolecular methods. The current results of ossicle shape identification could notdifferentiate the Holothuria from Stichopus species, at the species and genus level.Furthermore, the band position of the total genomic DNA extracts observed wasabove 10,000 base pair (bp). For Polymerase Chain Reaction (PCR), the annealingtemperatures for cyt-b and 165 ribosomal mtDNA primers are still being optimizedto date. It is believed that the findings from this study will provide insights that arerelevant for identifi cation efforts, morpholo gically and genetically.KEYWORDS: Stichopus, Holothuria, ossicles, mtDNA, cytochrome b, 165ribosomal mtDNA.31

Ossicle shapes and mitochondrial DNA profiles of holothuria and stichopus species

http://irep.iium.edu.my/12969/1/ossicle_shapes.pdf

Ossicle shapes and mitochondrial DNA profiles of holothuria and stichopus species

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