Amid, Azura

Ismail, Nurul Azira

English

The International Islamic University Malaysia Repository

TECHNICAL PROGRAMME&AbsTRACTs24th Symposium of Malaysian Chemical Engineers (SOMChE2010)15-17 June 2010Kuala Lumpur Convention CentreA Conference of ESTCONWorld Engineering, Science & Technology Congress2010IntErnatIOnal COnfErEnCE On prOCESS EnGInEErInG anD aDVanCED MatErIalS9 789832 271031ISBN 978-983-2271-03-12010 International Conference on process Engineering and advanced Materials(icpeam2010)technical programme & abstracts©	 2010,		 Universiti	Teknologi	PETRONAS	 Institute	of	Technology	PETRONAS	Sdn.	Bhd.Copyright and Reprint Permission:In	 compliance	 with	 the	 terms	 of	 the	 Copyright	 Act	 1987	 and	 the	 IP	 Policy	 of	 the	university,	 the	 copyright	 of	 this	 publication	 has	 been	 reassigned	 by	 the	 author	 to	 the	legal	 entity	 of	 the	 university,	 Institute	 of	 Technology	 PETRONAS	 Sdn.	 Bhd.	 	 Due	acknowledgement	shall	always	be	made	of	the	use	of	any	material	contained	in,	or	derived	from	 this	 publication.	 	 All	materials	 are	 copyright	 of	Universiti	 Teknologi	 PETRONAS.	Reproduction	 in	whole	 or	 in	 part	 is	 not	 permitted	without	 the	written	 permission	 from	the	University.a conference of World Engineering, Science & Technology Congress542010 International Conference on Process Engineering & Advanced Materialshigher	activation	rates	compared	to	other	activation	methods.	An	activation	time	of	5	hours	under	CO2	blanket	resulted	 in	higher	surface	area	and	pore	size.	Moreover,	it	was	shown	that	using	small	proportion	of	ZnCl2	and	H3PO4	creates	an	initial	narrow	microporosity.	 In	 this	 case	 further	physical	 activation	will	 grantee	 better	 development	 for	 the	 pore	 structure.	 In	terms	 of	 pore	 size	 distribution	 the	 combined	 preparation	method	resulted	 in	a	better	and	more	homogenous	pore	size	distribution	 than	 the	 commercial	 palm	 shell	 based	 activated	carbon.PEAO6_GTR_1	observation on recombinant Bromelain activity Before and after purification nurul azira ismail (international islamic university Malaysia (iiuM), Malaysia); azura amid (international islamic university Malaysia, Malaysia)Stem	 bromelain	 is	 a	 protease	 that	 commercially	 applied	 in	many	food	processing	industries	such	as	in	baking	and	cheese	production	and	also	as	a	meat	tenderizer.	It	is	also	demonstrates	both	 in vitro	 and	 in vivo	 therapeutic	 and	 pharmaceutical	properties.	Nowadays,	the	production	of	Bromelain	in	a	factory	consists	of	continuously	process	after	they	cleared	the	pineapple	fields.	However,	 it	 needs	 a	 high	 cost	 downstream	bioprocess	from	extraction,	purification	till	the	packaging	because	of	the	existence	of	protease	mixture	 in	 the	pineapple	waste	 and	 the	instability	of	the	Bromelain	itself.	Thus,	this	study	is	aims	to	use	recombinant	DNA	technology	in	order	to	obtain	a	consistent	yields	with	very	high	purity	and	activity	and	finally,	making	a	viable	 industrial	process	 feasible.	The	 recombinant	bromelain	gene	was	successfully	cloned	and	expressed	in	expression	vector	and	transformed	into	BL21-AI	cells.	An	active	and	functional	recombinant	 Bromelain	 was	 successfully	 produced	 after	purifiying	 it	using	the	affinity	chromatography	technique.	In	order	 to	 develop	 the	 best	 purification	 strategy,	 recombinant	Bromelain	activity	was	observed	before	and	after	purification.	The	protease	activity	of	recombinant	Bromelain	is	increased	as	the	protein	purified.	The	difference	in	activity	of	recombinant	Bromelain	was	discovered	between	the	protein	that	extracted	using	 native	 and	 denaturing	 conditions.	 The	 development	of	 purification	 strategies	 by	 determining	 the	 recombinant	Bromelain	activity	can	further	be	used	to	purify	the	large	scale	production	of	recombinant	Bromelain.PEAO6_GTR_2	identification of potential Biomarker in heart proteome for authentication of halal Slaughtered Chicken norshahida abu Samah (international islamic university Malaysia, Malaysia); azura amid (international islamic university Malaysia, Malaysia); Faridah yusof (international islamic university Malaysia, Malaysia)Nowadays,	 commercial	 slaughterhouses	 practice	 stunning	method	in	which	the	chickens	were	paralyzed	in	an	electrified	water	 bath	 prior	 to	 the	 slaughtering	 process.	 Such	 treatment	was	introduced	to	ease	up	and	fasten	the	slaughtering	process	in	 mass	 production.	 In	 Malaysia,	 the	 current	 for	 stunning	treatment	was	 only	 allowed	 to	 be	 in	 the	 range	 of	 0.25	A	 to	0.5	A.	However,	 there	 is	 no	method	 to	 identify	 the	 chicken	that	 has	 been	 slaughtered	 according	 to	 Halal	 requirement,	except	 through	 in-situ	 inspection	 by	 Department	 of	 Islamic	Development	 Malaysia	 (JAKIM)	 officer.	 In	 this	 study,	 a	simple	 and	 reliable	 method	 using	 proteomic	 approach	 has	been	 developed.	 Results	 showed	 that	 the	 sample	 collected	from	 chicken	 that	 slaughtered	 after	 over	 stunning	 contained	potential	protein	that	could	serve	as	biomarker	in	differentiating	the	 overdose	 stunning	 from	 the	 non-stunning	 and	 standard	stunning	of	low	voltage.	This	preliminary	study	encourage	for	further	detection	kit	development,	which	will	be	rapid,	simple	and	 reliable.	Then,	non-Halal,	over	 stunned	chickens	will	be	identified	even	if	they	are	already	in	supermarket.PEAO6_GTR_3	nanocrystalline Zeolite: a review niken Taufiqurrahmi (university Sains Malaysia, Malaysia); abdul Rahman Mohamed (universiti Sains Malaysia, Malaysia); Subhash bhatia (universiti Sains Malaysia, Malaysia)Nanocrystalline	zeolite	has	 received	 considerable	 attention	 in	the	 catalysis	 community.	 Zeolites	 with	 a	 crystal	 size	 smaller	than	100	nm	are	the	potential	replacement	for	existing	zeolite	catalysts	 due	 to	 its	 unique	 features	 with	 added	 advantages.	Several	research	groups	reported	synthesis	conditions	that	yield	different	 types	of	zeolites	 in	 small	 size	crystals.	The	decrease	in	 the	 crystal	 size	 produced	 changes	 in	 the	 physicochemical	properties	 of	 the	 zeolite	 compare	 to	microcrystalline	 zeolite.	This	 paper	 will	 focus	 on	 the	 synthesis	 and	 characterization	of	 nanocrystalline	 zeolite	 as	 catalysts.	 The	 application	 of	nanocrystalline	zeolite	 as	 catalyst	 in	 catalytic	 cracking	 is	 also	discussed.PEAO6_GTR_4	Effect of ph on the Biotransformation of (r)-1-(4-Bromo-phenyl)-Ethanol by Using aspergillus niger as a Biocatalyst Fatimatul abas (university Science of Malaysia, Malaysia); Mohamad Hekarl uzir (universiti Sains Malaysia, Malaysia); Mohd Mohd Zahar (STuDenT, Malaysia)Molecular	chirality	is	a	fundamental	phenomenon	that	plays	an	important	role	in	biological	processes.	Currently,	much	attention	has	been	focused	on	the	production	of	chiral	alcohols.	Chiral	alcohols	such	as	(R)-1-(4-bromo-phenyl)-ethanol	is	important	as	a	useful	starting	material	used	in	pharmaceutical	industries	for	drug	synthesis.	There	are	many	factors	that	influenced	the	bioconversion	which	some	of	the	crucial	ones	include	the	pH	of	the	media,	biotransformation	time,	substrate	concentration,	and	agitation	speed.	All	of	these	factors	significantly	affect	the	percentage	 of	 enantiomeric	 excess	 as	 well	 as	 the	 percentage	conversion	 of	 the	 chiral	 alcohol.	 In	 this	 work,	 biocatalytic	production	 of	 (R)-1-(4-bromo-phenyl)-ethanol	 was	 achieved	via	 asymmetric	 reduction	 of	 1-(4-bromo-phenyl)-ethanone	using	 the	 shake-flask	 method	 at	 the	 reaction	 conditions	 of	30o	C,	1mmol	of	substrate,	agitation	speed	at	150	rpm	and	at	various	pH’s.	Since	Aspergilus niger	can	easily	grow,	therefore,	it	was	used	as	a	biocatalyst	in	the	reaction.	Based	on	the	result	attained,	pH	7	gave	the	highest	of	enantiomeric	excess	(96%)	at	 the	 reduction	 time	 48	 hr.	Meanwhile	 the	 best	 conversion	ABSTRACTSoraL prESEnTaTionS

Observation on recombinant  bromelain activity before and after purification

http://irep.iium.edu.my/12271/1/Observation_on_Recombinant_Bromelain.pdf

Observation on recombinant bromelain activity before and after purification

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