Antifungal activity of Nepeta rtanjensis essential oil

Abstract

The chemical composition and antifungal activity of the essential oil of an endemic Serbian plant Nepeta rtanjensis Diklić & Milojević was studied. The essential oil was isolated from cultivated plants. Inhibition of mycelia growth of five micromycetes, two Alternaria species originally isolated from N. rtanjensis, Cladosporium cladosporoides, Trichoderma viride and Bipolaris spicifera, were tested using the agar dilution method. The essential oil of N. rtanjensis, the main component of which was 4aα,7α,7αβ-nepetalactone, showed strong antifungal activity against all the tested micromycetes. The minimum inhibitory concentration of N. rtanjensis essential oil ranged from 0.6 to 1.4 μg mL-1. The fungi most sensitive to the tested oil were Alternaria species, while Trichoderma viride was the most resistant.U ovom radu prezentovan je hemijski sastav i antifungalna aktivnost etarskog ulja endemične biljke Nepeta rtanjensis Diklić & Milojević. Etarsko ulje je izolovano iz kultivisanih biljaka. Inhibicija micelijalnog rasta pet mikromiceta, dve vrste roda Alternaria, izolovane sa N. rtanjensis, Cladosporium cladosporioides, Trichoderma viride i Bipolaris spicifera, testirana je makrodilucionom metodom. Etarsko ulje N. rtanjensis, čija je glavna komponenta 4aα,7α,7aβ-nepetalakton pokazuje jaku antifungalnu aktivnost u odnosu na sve testirane mikromicete. Minimalna inhibitorna koncentracija (MIC) etaskog ulja bila je u rasponu od 0,6 μg mL-1 do 1,4 μg mL-1. Najveću osetljivost na testirano ulje pokazale su vrste roda Alternaria dok je Trichoderma viride bila najotpornija.Projekat ministarstva br. 143041